Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,307	G→A	A56T (GCA→ACA)	ygiN →	putative quinol monooxygenase YgiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,307	0	G	A	98.8%	290.4 / ‑4.0	85	A56T (GCA→ACA)	ygiN	putative quinol monooxygenase YgiN
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (37/47); total (37/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

CTCGTCCTGGTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGT  >  NC_000913/3173170‑3173435

cTCGTCCTGGTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACa                                                                                                                                <  2:1015012/140‑1 (MQ=255)

  cGTCCTGGTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACAcc                                                                                                                              <  1:1023977/140‑1 (MQ=255)

   gTCCTGGTCAACATCACCGTCAGGCGGTATTTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACAcc                                                                                                                              >  2:331949/1‑140 (MQ=255)

     ccTGGTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGa                                                                                                                           <  1:278642/140‑1 (MQ=255)

        ggTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTc                                                                                                                        >  1:454047/1‑140 (MQ=255)

        ggTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTc                                                                                                                        >  1:706210/1‑140 (MQ=255)

        ggTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTc                                                                                                                        >  1:288323/1‑140 (MQ=255)

         gTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCt                                                                                                                       >  1:647479/1‑140 (MQ=255)

         gTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCt                                                                                                                       >  1:573656/1‑140 (MQ=255)

         gTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCt                                                                                                                       <  1:484988/140‑1 (MQ=255)

         gTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCt                                                                                                                       >  2:898355/1‑140 (MQ=255)

           cAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTAt                                                                                                                     <  1:469649/140‑1 (MQ=255)

             aCATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCC‑ATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGt                                                                                                                  <  1:813116/140‑1 (MQ=255)

              cATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGt                                                                                                                  <  1:252700/140‑1 (MQ=255)

              cATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGt                                                                                                                  <  1:625949/140‑1 (MQ=255)

              cATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGt                                                                                                                  >  2:106917/1‑140 (MQ=255)

                 cACCGTCAGGCGGTATTTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGtga                                                                                                                <  1:331949/140‑1 (MQ=255)

                   ccGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGtgatga                                                                                                             >  1:652771/1‑140 (MQ=255)

                    cGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTgatgat                                                                                                            >  2:170927/1‑140 (MQ=255)

                      tCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTg                                                                                                          >  1:527450/1‑140 (MQ=255)

                        aGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAg                                                                                                        >  1:323773/1‑140 (MQ=255)

                        aGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAg                                                                                                        >  2:845931/1‑140 (MQ=255)

                         ggCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGc                                                                                                       <  2:629352/140‑1 (MQ=255)

                         ggCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGc                                                                                                       <  2:803990/140‑1 (MQ=255)

                           aggTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAg                                                                                                     <  2:251986/139‑1 (MQ=255)

                                 ttGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGa                                                                                                <  1:379449/140‑1 (MQ=255)

                                       cAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCAt                                                                                          <  2:410114/140‑1 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  2:29694/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  2:1272300/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  1:653533/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  1:583256/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  1:110055/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  1:1084705/1‑140 (MQ=255)

                                         gTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                                                                                        >  1:247439/1‑140 (MQ=255)

                                          tttGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcgc                                                                                       <  2:838499/140‑1 (MQ=255)

                                          tttGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcgc                                                                                       >  1:762032/1‑140 (MQ=255)

                                               tAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATc                                                                                  >  2:666852/1‑140 (MQ=255)

                                                  aaTCGTTCCAACCGTACTGAAAGAAGAAGGATGCCACGGCTATGCGCCAATGGTGGATTGCGCCGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTg                                                                               >  1:557120/1‑140 (MQ=255)

                                                    tCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGaa                                                                             >  1:651865/1‑140 (MQ=255)

                                                     cGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAg                                                                            <  2:247439/140‑1 (MQ=255)

                                                     cGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAg                                                                            <  2:653533/140‑1 (MQ=255)

                                                     cGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAg                                                                            <  1:1105680/140‑1 (MQ=255)

                                                     cGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAg                                                                            <  2:485705/140‑1 (MQ=255)

                                                       ttCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg                                                                          <  2:448499/140‑1 (MQ=255)

                                                       ttCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg                                                                          <  1:617073/140‑1 (MQ=255)

                                                        tCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcgc                                                                         >  1:1098129/1‑140 (MQ=255)

                                                         ccAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCa                                                                        <  1:845931/140‑1 (MQ=255)

                                                            aCCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCt                                                                     >  2:778857/1‑140 (MQ=255)

                                                               gTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCa                                                                  <  1:747854/140‑1 (MQ=255)

                                                                   tGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAAcc                                                              <  2:762032/140‑1 (MQ=255)

                                                                     aaaGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAAcccc                                                            >  2:548229/1‑140 (MQ=255)

                                                                     aaaGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAAcccc                                                            <  2:693577/140‑1 (MQ=255)

                                                                       agaagaagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGc                                                          >  2:700103/1‑140 (MQ=255)

                                                                          agaagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGGGCATCTGCAAACCCCGcaca                                                       >  2:868494/1‑140 (MQ=255)

                                                                          agaagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGcact                                                       >  2:348612/1‑139 (MQ=255)

                                                                          agaagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGcaca                                                       >  1:289863/1‑140 (MQ=255)

                                                                          agaagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGcaca                                                       <  2:651865/140‑1 (MQ=255)

                                                                            aagGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATg                                                     <  2:940670/140‑1 (MQ=255)

                                                                               gTTGCCACTGCTATGCGCCAATGGTGGATTGCGCAGTTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAg                                                  >  1:1169836/1‑140 (MQ=255)

                                                                               gTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAg                                                  <  1:106917/140‑1 (MQ=255)

                                                                                 tGCCACGGCTATGCGCCAATGGTGGCTTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGc                                                >  2:1226010/1‑140 (MQ=255)

                                                                                        gCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGc                                         <  2:110055/140‑1 (MQ=255)

                                                                                          tATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGa                                       <  1:285277/140‑1 (MQ=255)

                                                                                          tATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGa                                       <  2:864200/140‑1 (MQ=255)

                                                                                           aTGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGaa                                      <  2:323773/140‑1 (MQ=255)

                                                                                           aTGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGaa                                      <  1:601927/140‑1 (MQ=255)

                                                                                           aTGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGaa                                      <  2:706210/140‑1 (MQ=255)

                                                                                                acAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGt                                 <  2:397750/139‑1 (MQ=255)

                                                                                                   aTGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTaaa                              >  2:307589/1‑140 (MQ=255)

                                                                                                      gtggATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACTCCGGATTCTGTCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGAGAAGCCGTAAAAgg                           <  2:9175/140‑1 (MQ=255)

                                                                                                      gtggATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAgg                           <  2:288323/140‑1 (MQ=255)

                                                                                                      gtggATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAgg                           <  2:454047/140‑1 (MQ=255)

                                                                                                        ggATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCGTCTTGAAGCGCATCTGCAAGCCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTg                         <  1:548229/140‑1 (MQ=255)

                                                                                                         gATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGa                        <  1:868494/140‑1 (MQ=255)

                                                                                                             gcgcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTg                    <  1:736424/140‑1 (MQ=255)

                                                                                                              cgcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGc                   >  1:1141139/1‑140 (MQ=255)

                                                                                                              cgcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGc                   >  2:309170/1‑140 (MQ=255)

                                                                                                              cgcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGc                   >  1:722325/1‑140 (MQ=255)

                                                                                                               ggagatGGCGTGAGTTCCCAGTCTATGACACCGGATTCTATCGTGATGATTGACCAGTGGGAAAACCTCGCGCATCTTGAAGCGCATCTGCTAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCt                  <  2:557120/135‑1 (MQ=255)

                                                                                                               gcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCt                  <  1:778857/140‑1 (MQ=255)

                                                                                                               gcAGCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCt                  <  2:1098129/140‑1 (MQ=255)

                                                                                                                  gCTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGga               <  1:624188/140‑1 (MQ=255)

                                                                                                                   cTGGCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGgag              >  2:601382/1‑140 (MQ=255)

                                                                                                                      gCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATg           <  1:307589/140‑1 (MQ=255)

                                                                                                                      gCGTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATg           <  1:29694/140‑1 (MQ=255)

                                                                                                                        gTGAGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGaa         <  1:666852/140‑1 (MQ=255)

                                                                                                                           aGTTTCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGGGATGAatat      <  1:218267/140‑1 (MQ=255)

                                                                                                                               tCCAGTCTATGACACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGt  <  1:1272300/140‑1 (MQ=255)

CTCGTCCTGGTCAACATCACCGTCAGGCGGTA‑TTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGT  >  NC_000913/3173170‑3173435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A