Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F48 I1 R1 70 76.3 2572620 98.7% 2539175 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,296,076 G→T A90S (GCC→TCC)  diaA → DnaA initiator‑associating factor for replication initiation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,296,0760GT97.3% 250.7 / ‑0.8 73coding (268/591 nt)diaADnaA initiator‑associating factor for replication initiation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (36/35);  minor base . (1/1);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

TCTGTTGTGGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTGCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGC  >  NC_000913/3295942‑3296211
                                                                                                                                           |                                                                                                                                       
tctGTTGTGGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAAc                                                                                                                                    <  2:839353/140‑1 (MQ=255)
 ctGTTGTAGTAATGGAACTTGCGCTGCCGAT‑‑‑‑‑GATCAACATTTTGCTGCCAGTATGAAACACTGTTTGGCAGGGGTGGGGACTTGCTTCACTCAATTTGTACTAAATTCTGATAATGTTGTCTTAACGGCGATTTCCAACg                                                                                                                                   <  2:858030/140‑1 (MQ=255)
 atgttgtGGTAATGGAACTTCCGCGGCCAAT‑‑‑‑‑GCCCAGCATTTTGCTGCCAGCATTATCAACCGTTTCGAAACGGAGCGGCCCAGCGTGCCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACg                                                                                                                                   <  2:664894/139‑1 (MQ=255)
   gttgtGGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAAAGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGAt                                                                                                                                 <  2:1054086/140‑1 (MQ=255)
      gtgGTAATGGAACTTCCGCTGCCAATGCACAGCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACg                                                                                                                                   <  2:23797/140‑1 (MQ=255)
      gtgGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGc                                                                                                                              <  2:1122004/140‑1 (MQ=255)
      gtgGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGc                                                                                                                              <  2:409709/140‑1 (MQ=255)
        ggTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCtt                                                                                                                            <  1:1242966/140‑1 (MQ=255)
        ggTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCACCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCtt                                                                                                                            <  2:1000266/140‑1 (MQ=255)
        ggTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGATTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCtt                                                                                                                            <  2:967179/140‑1 (MQ=255)
           aaTGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACa                                                                                                                         <  1:295188/140‑1 (MQ=255)
              ggAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACatga                                                                                                                      >  2:903668/1‑140 (MQ=255)
               gAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACatgat                                                                                                                     <  1:933129/140‑1 (MQ=255)
               gAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACatgat                                                                                                                     <  2:937086/140‑1 (MQ=255)
                aaCTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACatgatg                                                                                                                    <  2:257189/140‑1 (MQ=255)
                  cTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGaa                                                                                                                  <  1:434398/140‑1 (MQ=255)
                    tCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAgt                                                                                                                <  1:290362/140‑1 (MQ=255)
                                          gCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGc                                                                                               >  1:180018/1‑140 (MQ=255)
                                          gCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGc                                                                                               >  2:18793/1‑140 (MQ=255)
                                          gCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATT‑CCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCg                                                                                              >  1:19080/1‑140 (MQ=255)
                                           cATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCg                                                                                              >  2:134808/1‑140 (MQ=255)
                                              tttGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGgc                                                                                           >  2:1015772/1‑140 (MQ=255)
                                               ttGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCCGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGCTCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGgcg                                                                                          >  2:84530/1‑140 (MQ=255)
                                               ttGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGgcg                                                                                          <  2:601560/140‑1 (MQ=255)
                                                     ccAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGt                                                                                    >  2:560408/1‑140 (MQ=255)
                                                     ccAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGt                                                                                    >  1:651883/1‑140 (MQ=255)
                                                       aGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCa                                                                                  <  1:136990/140‑1 (MQ=255)
                                                         cATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATg                                                                                <  1:547989/140‑1 (MQ=255)
                                                          aTGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGc                                                                               <  1:1026928/140‑1 (MQ=255)
                                                            gATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCgg                                                                             <  1:18793/140‑1 (MQ=255)
                                                            gATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCgg                                                                             <  2:177571/140‑1 (MQ=255)
                                                             aTCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCggg                                                                            >  1:1066888/1‑140 (MQ=255)
                                                                 aCCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACTGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGAt                                                                        >  2:255052/1‑140 (MQ=255)
                                                                  ccGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATg                                                                       <  2:839284/140‑1 (MQ=255)
                                                                   cGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGt                                                                      >  2:827421/1‑140 (MQ=255)
                                                                   cGTTCCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGt                                                                      <  1:400489/140‑1 (MQ=255)
                                                                    gTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTa                                                                     >  1:293114/1‑140 (MQ=255)
                                                                      ttCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGAt                                                                        <  1:1009529/135‑1 (MQ=255)
                                                                      ttCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGAt                                                                        >  2:1009529/1‑135 (MQ=255)
                                                                       tCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCTATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTAttt                                                                  >  2:1073133/1‑139 (MQ=255)
                                                                       tCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTg                                                                  >  1:651599/1‑140 (MQ=255)
                                                                                  cGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTc                                                       <  1:560408/140‑1 (MQ=255)
                                                                                       aaGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCAccc                                                  <  2:1248749/139‑1 (MQ=255)
                                                                                          cTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTg                                               >  2:989125/1‑140 (MQ=255)
                                                                                               cTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAAc                                          >  1:16259/1‑140 (MQ=255)
                                                                                               cTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAAc                                          >  1:732718/1‑140 (MQ=255)
                                                                                                tGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATT‑CCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAg                                        <  2:19080/140‑1 (MQ=255)
                                                                                                 gCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAg                                        <  2:180018/140‑1 (MQ=255)
                                                                                                  ccATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGc                                       >  2:218889/1‑140 (MQ=255)
                                                                                                   cATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGcc                                      >  1:117977/1‑140 (MQ=255)
                                                                                                    aTTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCcg                                     >  1:199929/1‑140 (MQ=255)
                                                                                                    aTTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCcg                                     >  1:1055018/1‑140 (MQ=255)
                                                                                                    aTTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCcg                                     <  1:930928/140‑1 (MQ=255)
                                                                                                      ttgcattaaTACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCcgcg                                   <  1:156033/133‑1 (MQ=255)
                                                                                                        cACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGat                                 <  1:1243904/140‑1 (MQ=255)
                                                                                                        cACTAAATACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGat                                 <  2:651883/140‑1 (MQ=255)
                                                                                                             aaTACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGt                            >  1:219255/1‑140 (MQ=255)
                                                                                                             aaTACTGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGt                            >  1:280352/1‑140 (MQ=255)
                                                                                                                  tGATAATGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAg                       <  2:280352/140‑1 (MQ=255)
                                                                                                                       aTGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGtt                  >  1:636337/1‑140 (MQ=255)
                                                                                                                       aTGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGtt                  >  1:624621/1‑140 (MQ=255)
                                                                                                                       aTGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGtt                  >  2:641446/1‑140 (MQ=255)
                                                                                                                       aTGTTGTCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGtt                  >  2:430994/1‑140 (MQ=255)
                                                                                                                         gttgtCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGa                >  2:126939/1‑140 (MQ=255)
                                                                                                                         gttgtCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGa                >  1:640380/1‑140 (MQ=255)
                                                                                                                         gttgtCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGa                >  2:664302/1‑140 (MQ=255)
                                                                                                                            gtCTTAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAgc             >  2:1062750/1‑140 (MQ=255)
                                                                                                                               ttAACGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAgccgc          >  1:599823/1‑140 (MQ=255)
                                                                                                                                  aCGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGt       >  2:534415/1‑140 (MQ=255)
                                                                                                                                   cGGCGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGtt      >  2:301616/1‑140 (MQ=255)
                                                                                                                                      cGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTAcg   <  1:134808/140‑1 (MQ=255)
                                                                                                                                      cGATTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTAcg   <  2:636337/140‑1 (MQ=255)
                                                                                                                                       gATTTCCAACGGTCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCTCGATATTGTTAAAGCAGTTGAAGCCGCCGTTAcgc  >  2:1010837/1‑140 (MQ=255)
                                                                                                                                        aTTTCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTa     <  1:1108486/136‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TCTGTTGTGGTAATGGAACTTCCGCTGCCAAT‑‑‑‑‑GCACAGCATTTTGCTGCCAGCATGATCAACCGTTTCGAAACGGAGCGGCCCAGCTTACCTGCCATTGCACTAAATACTGATAATGTTGTCTTAACGGCGATTGCCAACGATCGCTTACATGATGAAGTGTATGCAAAACAGGTGCGGGCGCTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGC  >  NC_000913/3295942‑3296211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A