Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F48 I1 R1 70 76.3 2572620 98.7% 2539175 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,228,142 G→T G138W (GGG→TGG)  yjbB → putative inorganic phosphate export protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,228,1420GT95.1% 200.8 / 4.9 61G138W (GGG→TGG) yjbBputative inorganic phosphate export protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (26/32);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01

ATGGCGCGTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGCGCGATGTTCGC  >  NC_000913/4228016‑4228275
                                                                                                                              |                                                                                                                                     
aTGGCGCGTATTCTCACCTTCGCCTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTAGTCTTTGGCTGATTTTGCt                                                                                                                          <  1:871436/140‑1 (MQ=255)
  ggCGCGTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAg                                                                                                                        <  1:1238912/140‑1 (MQ=255)
    cgcgTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAgcg                                                                                                                      <  1:1179431/140‑1 (MQ=255)
    cgcgTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAgcg                                                                                                                      <  1:731503/140‑1 (MQ=255)
     gcgTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAgcgc                                                                                                                     <  2:1036940/140‑1 (MQ=255)
      cgTATTCTCACCTTCGACTTATCCTGGCTGTCATCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCt                                                                                                                    <  2:615380/140‑1 (MQ=255)
      cgTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCt                                                                                                                    <  2:1043934/140‑1 (MQ=255)
                   tCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGGATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATtgtg                                                                                                       >  1:808548/1‑140 (MQ=255)
                    cGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGc                                                                                                      >  2:1142328/1‑140 (MQ=255)
                    cGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGc                                                                                                      >  2:1103601/1‑140 (MQ=255)
                     gACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCa                                                                                                     <  1:1103601/140‑1 (MQ=255)
                     gACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCa                                                                                                     <  1:1121037/140‑1 (MQ=255)
                          aTCCTGGCTGTCACCGTTACTTATTTTTATAGGCGTGATTTTTTCCCTCGGACGCAACCAGTCCCGGGCCGGGCACCTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCg                                                                                                <  1:1240183/140‑1 (MQ=255)
                           tCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGt                                                                                               >  1:747528/1‑140 (MQ=255)
                           tCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGt                                                                                               >  1:932900/1‑140 (MQ=255)
                           tCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGt                                                                                               >  1:505775/1‑140 (MQ=255)
                             cTGGCTGTCGCCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTaa                                                                                             <  2:954282/140‑1 (MQ=255)
                             cTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTaa                                                                                             <  2:311775/140‑1 (MQ=255)
                             cTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGGCACGCGCCGGGCAACTGGGCCGCGGCGGGATTGGGCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTTTGCAGGCCGTaa                                                                                             >  1:1007608/1‑140 (MQ=255)
                                gCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGGATTGGTCTTTGGCTGATTTTTCTAGCGCTGGTGTTGATTGTGCAGGCCGTAACGc                                                                                          >  1:8223/1‑140 (MQ=255)
                                    tCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGGATTTGGCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGAt                                                                                      >  1:41799/1‑140 (MQ=255)
                                      aCCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGGCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCa                                                                                    >  2:1066174/1‑140 (MQ=255)
                                        cGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCAcc                                                                                  <  2:505775/140‑1 (MQ=255)
                                          ttACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCa                                                                                >  1:880719/1‑140 (MQ=255)
                                              ttATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCa                                                                            >  1:168408/1‑140 (MQ=255)
                                                aTTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCaaa                                                                          <  2:1240479/140‑1 (MQ=255)
                                                  ttttATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACg                                                                        >  2:906917/1‑140 (MQ=255)
                                                      aTCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGGGTCGGGATTGGGCTTTGGCTGATTTTGCTAGCGCTGGGGTTGATTGGGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGt                                                                    >  1:585757/1‑140 (MQ=255)
                                                      aTCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGt                                                                    <  2:747528/140‑1 (MQ=255)
                                                       tCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGtt                                                                   <  1:345275/140‑1 (MQ=255)
                                                          gCGTGATTTTTTTTCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGGCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAg                                                                >  1:891138/1‑140 (MQ=255)
                                                          gCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAg                                                                >  1:220354/1‑140 (MQ=255)
                                                           cGTGATTTTTTTCCTCGTACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTCGTCATTGGCTGATTTTGCTAGCGCCGGGGATGATTTTGCAGGCCGTAACCCCGGTTATCCCGGCAAACCGCGTTCAgg                                                               >  2:716697/1‑140 (MQ=255)
                                                           cGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAgg                                                               >  1:1149706/1‑140 (MQ=255)
                                                            gTGATTTTTTTCCTCGGACGCAATCAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGt                                                              <  2:1025270/140‑1 (MQ=255)
                                                            gTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGACCACCTAGGCAAACGGCGTTCAGGt                                                              <  1:1142328/140‑1 (MQ=255)
                                                            gTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTAGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGt                                                              <  2:971765/140‑1 (MQ=255)
                                                                   ttttCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCttt                                                       <  1:663774/140‑1 (MQ=255)
                                                                    tttCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTg                                                      >  1:612370/1‑140 (MQ=255)
                                                                    tttCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTg                                                      <  2:168408/140‑1 (MQ=255)
                                                                                      gTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGatt                                    >  2:429414/1‑139 (MQ=255)
                                                                                       tCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGatat                                   >  2:1176456/1‑140 (MQ=255)
                                                                                        cACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATAtt                                  >  1:1203714/1‑140 (MQ=255)
                                                                                          cgcgCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTct                                <  1:37260/140‑1 (MQ=255)
                                                                                          cgcgCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTct                                <  2:932900/140‑1 (MQ=255)
                                                                                          agcgcCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTct                                <  2:219145/139‑1 (MQ=255)
                                                                                           gcgcCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTctg                               <  1:467023/140‑1 (MQ=255)
                                                                                           gcgcCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTctg                               <  2:1149706/140‑1 (MQ=255)
                                                                                               cGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTgg                           >  1:881144/1‑140 (MQ=255)
                                                                                               cGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTgg                           >  1:1216300/1‑140 (MQ=255)
                                                                                                 ggCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGAt                         <  2:585757/140‑1 (MQ=255)
                                                                                                         ggCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACTGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGAt                 >  1:105108/1‑140 (MQ=255)
                                                                                                         ggCCGCGTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGAt                 >  1:16094/1‑140 (MQ=255)
                                                                                                         ggCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGAt                 >  2:745831/1‑140 (MQ=255)
                                                                                                              cgTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGgcg            <  2:612370/140‑1 (MQ=255)
                                                                                                              cgTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGgcg            >  2:694009/1‑140 (MQ=255)
                                                                                                              cgTCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGgcg            <  1:495207/140‑1 (MQ=255)
                                                                                                                tCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGTGCTGATTGGcgcg          <  2:465779/140‑1 (MQ=255)
                                                                                                                tCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGcgcg          <  2:220354/140‑1 (MQ=255)
                                                                                                                tCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGcgcg          <  2:880719/140‑1 (MQ=255)
                                                                                                                tCGGTATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGcgcg          <  2:891138/140‑1 (MQ=255)
                                                                                                                    tATTGGTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGCGCGATGt      <  1:712291/140‑1 (MQ=255)
                                                                                                                        ggTCTTTGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGCGCGATGTTCGc  <  1:180362/140‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
ATGGCGCGTATTCTCACCTTCGACTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACCCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACCGGCGATATTCTGCTGGATGCGCTGATTGGCGCGATGTTCGC  >  NC_000913/4228016‑4228275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A