Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,323,993	C→A	W120C (TGG→TGT)	phnD ←	phosphonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,323,993	0	C	A	98.5%	233.2 / ‑2.9	67	W120C (TGG→TGT)	phnD	phosphonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (44/22); total (45/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATG  >  NC_000913/4323870‑4324128

gCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatc                                                                                                                         >  2:861843/1‑140 (MQ=255)

 ccGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatcc                                                                                                                        >  2:989286/1‑140 (MQ=255)

 ccGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatcc                                                                                                                        >  2:570917/1‑140 (MQ=255)

  cGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatcca                                                                                                                       >  2:784796/1‑140 (MQ=255)

    gggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatccatc                                                                                                                     >  2:718468/1‑140 (MQ=255)

     ggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatccatcc                                                                                                                    >  2:543978/1‑140 (MQ=255)

     ggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGatccatcc                                                                                                                    >  2:163838/1‑140 (MQ=255)

        cGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATccgcc                                                                                                                 <  2:949435/140‑1 (MQ=255)

        cGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATccgcc                                                                                                                 >  1:566539/1‑140 (MQ=255)

           ggAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCcgcg                                                                                                              >  2:1197233/1‑140 (MQ=255)

                ccAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGt                                                                                                         >  1:44199/1‑140 (MQ=255)

                 aagagGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTc                                                                                                        <  2:502535/139‑1 (MQ=255)

                         gAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGaa                                                                                                >  1:458922/1‑140 (MQ=255)

                            ttAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAc                                                                                             <  2:619107/140‑1 (MQ=255)

                            ttAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAc                                                                                             <  2:13783/140‑1 (MQ=255)

                              aGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCt                                                                                           <  1:377914/140‑1 (MQ=255)

                              aGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCt                                                                                           >  1:1051129/1‑140 (MQ=255)

                                gATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTgg                                                                                         >  1:255913/1‑140 (MQ=255)

                                 aTCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGc                                                                                        >  1:551457/1‑140 (MQ=255)

                                    gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAt                                                                                     <  1:117038/140‑1 (MQ=255)

                                    gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAt                                                                                     >  2:602034/1‑140 (MQ=255)

                                    gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAt                                                                                     >  1:671024/1‑140 (MQ=255)

                                          gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGgcgc                                                                               <  1:163838/140‑1 (MQ=255)

                                          gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGgcgc                                                                               <  1:543978/140‑1 (MQ=255)

                                          gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGgc                                                                                 <  1:934333/138‑1 (MQ=255)

                                          gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGgc                                                                                 >  2:934333/1‑138 (MQ=255)

                                                   gagaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCg                                                                      >  1:77337/1‑140 (MQ=255)

                                                    agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCgt                                                                     >  1:35745/1‑139 (MQ=255)

                                                          ttCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCAt                                                               >  1:359039/1‑140 (MQ=255)

                                                           tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATc                                                              >  2:2073/1‑140 (MQ=255)

                                                           tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATc                                                              >  1:1144527/1‑140 (MQ=255)

                                                           tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATc                                                              >  1:328918/1‑140 (MQ=255)

                                                           tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATc                                                              >  1:585328/1‑140 (MQ=255)

                                                            ccGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCg                                                             >  2:175821/1‑140 (MQ=255)

                                                                 tCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGAc                                                        >  1:1211818/1‑140 (MQ=255)

                                                                 tCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGAc                                                        >  2:876161/1‑140 (MQ=255)

                                                                  cGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACa                                                       >  1:102525/1‑140 (MQ=255)

                                                                  cGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCATCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACa                                                       >  1:565765/1‑140 (MQ=255)

                                                                   gCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAg                                                      <  2:1051129/140‑1 (MQ=255)

                                                                                   ggTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTTGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCg                                      >  2:1106126/1‑140 (MQ=255)

                                                                                   ggTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCg                                      <  1:718468/140‑1 (MQ=255)

                                                                                     ttgttgATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGat                                    <  2:328918/140‑1 (MQ=255)

                                                                                       gttgATCGGACTGTCTTTGTTGACGATCAACACGATACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGatat                                  <  1:593514/140‑1 (MQ=255)

                                                                                         tgATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATcc                                >  2:491392/1‑140 (MQ=255)

                                                                                          gATCGGACTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGACCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCa                               <  1:784796/140‑1 (MQ=255)

                                                                                                aCTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTAt                         >  1:689989/1‑140 (MQ=255)

                                                                                                aCTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTAt                         >  1:1259523/1‑140 (MQ=255)

                                                                                                aCTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTAt                         >  2:1066036/1‑140 (MQ=255)

                                                                                                 cTGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTAtt                        >  1:231423/1‑140 (MQ=255)

                                                                                                  tGTCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTg                       >  1:119539/1‑140 (MQ=255)

                                                                                                    tCTTTGTTGACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGaa                     >  1:829294/1‑140 (MQ=255)

                                                                                                        tgttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc                 <  2:742989/140‑1 (MQ=255)

                                                                                                        tgttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc                 <  2:255913/140‑1 (MQ=255)

                                                                                                        tgttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc                 <  1:1197233/140‑1 (MQ=255)

                                                                                                        tgttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc                 <  2:551457/140‑1 (MQ=255)

                                                                                                        tgttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAATGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc                 <  2:1144527/140‑1 (MQ=255)

                                                                                                         tttgCCGATCAACACGCTACAGTAACCCGGCAATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCa                <  2:35745/139‑1 (MQ=255)

                                                                                                          ttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAt               >  2:1009800/1‑140 (MQ=255)

                                                                                                          ttgACGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAt               >  2:493564/1‑140 (MQ=255)

                                                                                                             aCGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATccc            >  2:5411/1‑140 (MQ=255)

                                                                                                             aCGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCACTTCCATCGCCGACAGATTGCCGTACCAGGAGATATCCACTTTATTGAAGCGCATccc            <  1:1044674/140‑1 (MQ=255)

                                                                                                              cGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATcccc           >  2:45321/1‑140 (MQ=255)

                                                                                                              cGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATcccc           >  2:415310/1‑140 (MQ=255)

                                                                                                              cGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATcccc           >  2:138364/1‑140 (MQ=255)

                                                                                                              cGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATcccc           >  1:685157/1‑140 (MQ=255)

                                                                                                              cGATCAACACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATcccc           >  1:1117204/1‑140 (MQ=255)

                                                                                                                   aaCACGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGAt      <  2:44199/140‑1 (MQ=255)

                                                                                                                       cGCTACAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCACCCCCTGGATAATg  <  1:570917/140‑1 (MQ=255)

GCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGATCCACCGCTTCCATCGCCGACAGATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATG  >  NC_000913/4323870‑4324128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A