Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,196	C→T	L88L (CTG→TTG)	fklB →	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,196	0	C	T	100.0%	197.8 / NA	56	L88L (CTG→TTG)	fklB	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/29); total (27/29)

TTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGAT  >  NC_000913/4429059‑4429329

ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTg                                                                                                                                     >  1:126073/1‑140 (MQ=255)

ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTg                                                                                                                                     >  2:562823/1‑140 (MQ=255)

  gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGga                                                                                                                                   >  1:962143/1‑140 (MQ=255)

       tGCGCTGGAAGGCAAACATCCGGCTGTTCCGGGTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGCTTCCAGGCGATGGCTGCTGAAGGTGCGAAATACTTGGAAGaaa                                                                                                                              >  2:664928/1‑140 (MQ=255)

         cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGATATCCCAGAGCGCGCCGATGGCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGTTGTGAAATACTTGGAAGAAAAc                                                                                                                            <  2:255099/140‑1 (MQ=255)

          gcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACg                                                                                                                           <  1:16007/140‑1 (MQ=255)

            tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGcc                                                                                                                         <  2:1054549/140‑1 (MQ=255)

              gAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCaa                                                                                                                       <  2:56678/140‑1 (MQ=255)

                      aCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAaagaag                                                                                                               <  2:1136837/140‑1 (MQ=255)

                      aCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAaagaag                                                                                                               <  1:804815/140‑1 (MQ=255)

                        aTCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGgt                                                                                                             >  2:394085/1‑140 (MQ=255)

                            ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGa                                                                                                         >  2:850427/1‑140 (MQ=255)

                             gCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                                                                        <  2:160635/140‑1 (MQ=255)

                                  tCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCa                                                                                                   >  2:835808/1‑140 (MQ=255)

                                   ccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCAc                                                                                                  <  2:750450/140‑1 (MQ=255)

                                           tgtgGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                                                                          <  2:54884/140‑1 (MQ=255)

                                           tgtgGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                                                                          <  2:5399/140‑1 (MQ=255)

                                           tgtgGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                                                                          <  2:934000/140‑1 (MQ=255)

                                                tgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTg                                                                                     >  1:1012086/1‑140 (MQ=255)

                                                       cgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTcc                                                                              >  2:967208/1‑140 (MQ=255)

                                                         cgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgc                                                                            >  1:1096713/1‑140 (MQ=255)

                                                          gcTGCGTGAAATCCACGAGCGCGTTGATGCCGTTCGTGGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAACTGCTTGGAGGTAAACACCAAAAAAGAGGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgcg                                                                           <  2:356933/140‑1 (MQ=255)

                                                              cGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                                                                       >  1:856665/1‑140 (MQ=255)

                                                              cGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                                                                       >  1:624605/1‑140 (MQ=255)

                                                                  aaaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                   <  1:544337/140‑1 (MQ=255)

                                                                  aaaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                   <  1:867439/140‑1 (MQ=255)

                                                                  aaaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                   <  1:772695/140‑1 (MQ=255)

                                                                  aaaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                   <  2:910042/140‑1 (MQ=255)

                                                                    aTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa                                                                 <  1:609727/140‑1 (MQ=255)

                                                                     tCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg                                                                <  2:126073/140‑1 (MQ=255)

                                                                        aCGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGt                                                             >  2:371785/1‑140 (MQ=255)

                                                                        aCGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGt                                                             >  1:173710/1‑140 (MQ=255)

                                                                               cgcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcg                                                      >  2:709017/1‑140 (MQ=255)

                                                                                    aTGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                 <  2:1160415/140‑1 (MQ=255)

                                                                                    aTGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                 <  2:806880/140‑1 (MQ=255)

                                                                                        cGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCgg                                             <  1:394085/140‑1 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccg                                    >  1:681287/1‑140 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccg                                    >  2:32227/1‑140 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccg                                    >  1:1157951/1‑140 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccg                                    >  1:583158/1‑140 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGCGAAGGCGCAATTCCGGCACGTaccg                                    >  1:84775/1‑140 (MQ=255)

                                                                                                 tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTAGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccg                                    >  1:538758/1‑140 (MQ=255)

                                                                                                  cAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccga                                   >  1:92009/1‑140 (MQ=255)

                                                                                                   aGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccgac                                  <  1:232289/140‑1 (MQ=255)

                                                                                                     ggTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccgaccg                                <  2:1012854/139‑1 (MQ=255)

                                                                                                     cGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccgaccg                                >  1:391077/1‑140 (MQ=255)

                                                                                                        ttCCAGGCGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGt                             <  1:562823/140‑1 (MQ=255)

                                                                                                               cGATGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGtt                      >  1:1179859/1‑140 (MQ=255)

                                                                                                                  tGGCTGCTGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCAt                   >  1:856540/1‑140 (MQ=255)

                                                                                                                    gctgctGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                 >  2:451823/1‑140 (MQ=255)

                                                                                                                    gctgctGAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                 <  1:835808/140‑1 (MQ=255)

                                                                                                                       gctgAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTacac              <  2:681287/140‑1 (MQ=255)

                                                                                                                        ctgAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACAcc             <  2:962143/140‑1 (MQ=255)

                                                                                                                         tgAAGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCg            <  2:986772/140‑1 (MQ=255)

                                                                                                                            aGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTa         <  2:1648/140‑1 (MQ=255)

                                                                                                                            aGGTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTa         <  2:1096713/140‑1 (MQ=255)

                                                                                                                             ggTGTGAAATACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTaa        >  2:483650/1‑140 (MQ=255)

                                                                                                                               tgtgAAATACTTGGAAGAAAACGCCTAAAAAGTAGGTGTGCATAGCACCGACTCTGGCCTGCAATTCCGCGTGCTCAATTAGGGTGAAGGCGCAAATCCGGCACGTACCGATCGCGCTGGCGTTCATTACACCGTTacat      >  2:1004696/1‑137 (MQ=255)

                                                                                                                                   aaaTACTTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGAt  >  1:248332/1‑140 (MQ=255)

TTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGAT  >  NC_000913/4429059‑4429329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A