Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F48 I1 R1 61 74.3 2613450 99.1% 2589928 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,395,064 C→A D399Y (GAT→TAT)  yhdP ← AsmA2 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,395,0640CA100.0% 153.7 / NA 44D399Y (GAT→TAT) yhdPAsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/22);  total (22/22)

CACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTCGCGGCCAG  >  NC_000913/3394929‑3395196
                                                                                                                                       |                                                                                                                                    
cACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTc                                                                                                                                  >  2:383753/1‑140 (MQ=255)
            tttCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGa                                                                                                                      >  2:1063833/1‑140 (MQ=255)
            tttCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGa                                                                                                                      >  2:1065557/1‑140 (MQ=255)
             ttCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGaa                                                                                                                     >  1:53848/1‑140 (MQ=255)
               cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGaaaa                                                                                                                   >  2:1261696/1‑140 (MQ=255)
               cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGaaaa                                                                                                                   >  2:414301/1‑140 (MQ=255)
               cGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGaaaa                                                                                                                   >  2:127690/1‑140 (MQ=255)
                         cTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGt                                                                                                         >  2:948765/1‑140 (MQ=255)
                           ttGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCt                                                                                                       <  1:485243/140‑1 (MQ=255)
                            tGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTg                                                                                                      <  1:127690/140‑1 (MQ=255)
                              acTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCTTGAAAACGGGTCTTGTCTgcc                                                                                                    <  2:1006631/139‑1 (MQ=255)
                                tGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTgccgc                                                                                                  <  1:1084557/140‑1 (MQ=255)
                                 gCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTgccgcc                                                                                                 <  1:383753/140‑1 (MQ=255)
                                  cTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCt                                                                                                >  2:1019185/1‑140 (MQ=255)
                                     cATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGaa                                                                                             <  2:465780/140‑1 (MQ=255)
                                     cATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGaa                                                                                             <  2:673793/140‑1 (MQ=255)
                                          aCGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCggg                                                                                        <  1:97192/140‑1 (MQ=255)
                                                    cAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAgcg                                                                              <  1:921728/140‑1 (MQ=255)
                                                       aCCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCa                                                                           <  2:553210/140‑1 (MQ=255)
                                                        ccATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCag                                                                          <  1:948765/140‑1 (MQ=255)
                                                                   gCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCt                                                               <  1:1019185/140‑1 (MQ=255)
                                                                     tGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCttt                                                             >  2:1047005/1‑139 (MQ=255)
                                                                     tGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTg                                                             >  1:335504/1‑140 (MQ=255)
                                                                        cGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCg                                                          <  1:794637/140‑1 (MQ=255)
                                                                          gAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCt                                                        <  1:598904/140‑1 (MQ=255)
                                                                          gAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCt                                                        <  2:365512/140‑1 (MQ=255)
                                                                             aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCgg                                                     <  2:53848/140‑1 (MQ=255)
                                                                                 tCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTtgt                                                 >  2:1235542/1‑140 (MQ=255)
                                                                                    cGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTtgtgtg                                              <  2:335504/140‑1 (MQ=255)
                                                                                    cGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTtgtgtg                                              <  1:1065557/140‑1 (MQ=255)
                                                                                       agaAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAg                                           <  1:1063833/140‑1 (MQ=255)
                                                                                          aGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAgcgc                                        >  2:346874/1‑140 (MQ=255)
                                                                                           gtgtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGcc                                       <  1:414301/140‑1 (MQ=255)
                                                                                                 cGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACAt                                 >  2:225246/1‑140 (MQ=255)
                                                                                                     cccGGTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCAcc                             <  1:1235542/140‑1 (MQ=255)
                                                                                                        ggTAATAATTTCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAg                          >  1:933112/1‑140 (MQ=255)
                                                                                                                  tCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGaa                >  2:641503/1‑140 (MQ=255)
                                                                                                                  tCCATTGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGaa                >  1:1150208/1‑140 (MQ=255)
                                                                                                                    cATTGCTTCCAGGCCAGATAGCTCCACGGTGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAg              >  1:724868/1‑140 (MQ=255)
                                                                                                                       tGCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGttt           >  1:956356/1‑140 (MQ=255)
                                                                                                                        gCTTCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTc          >  1:247360/1‑140 (MQ=255)
                                                                                                                           tCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTcgcg       >  1:902022/1‑140 (MQ=255)
                                                                                                                           tCCAGGCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTcgcg       >  2:727699/1‑140 (MQ=255)
                                                                                                                                gCCAGATAGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTCGCGGCCAg  <  1:1047005/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CACGGAATACCGTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCCGGAAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCCAGCGCCAGAGTGTTAATCTTGCCGCTCGGTTGTGTGGAGCGCCAAACATCACCCAGTGCAGGTGAAAGTTTCGCGGCCAG  >  NC_000913/3394929‑3395196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A