Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F48 I1 R1	61	74.3	2613450	99.1%	2589928	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,142,527	C→A	intergenic (‑306/‑3)	ptsA ← / → frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,527	0	C	A	100.0%	217.2 / NA	61	intergenic (‑306/‑3)	ptsA/frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (30/31); total (30/31)

GGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAG  >  NC_000913/4142391‑4142662

ggCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTa                                                                                                                                      <  1:1185893/140‑1 (MQ=255)

    gATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTatgaa                                                                                                                                  <  2:916009/140‑1 (MQ=255)

    gATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTatgaa                                                                                                                                  >  2:897322/1‑140 (MQ=255)

    gATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTatgaa                                                                                                                                  <  2:1260944/140‑1 (MQ=255)

        gCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAg                                                                                                                              <  1:570302/140‑1 (MQ=255)

        gCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAg                                                                                                                              <  2:258419/140‑1 (MQ=255)

         gaTTTTTGTACTAATATTACAAAAGTCCAATAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGt                                                                                                                             <  2:1282663/139‑1 (MQ=255)

         cATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGt                                                                                                                             >  1:198466/1‑140 (MQ=255)

           tttttGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTg                                                                                                                           >  1:601184/1‑140 (MQ=255)

               tGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGc                                                                                                                       <  2:20613/140‑1 (MQ=255)

                    tGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATc                                                                                                                  <  1:641245/140‑1 (MQ=255)

                     gATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGTACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATcc                                                                                                                 <  2:1240225/140‑1 (MQ=255)

                      atatTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCt                                                                                                                >  2:924631/1‑140 (MQ=255)

                           aCAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGaaaa                                                                                                           >  1:1018694/1‑140 (MQ=255)

                           aCAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGaaaa                                                                                                           >  1:1273606/1‑140 (MQ=255)

                                aGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc                                                                                                      >  1:974038/1‑140 (MQ=255)

                                aGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc                                                                                                      >  2:784296/1‑140 (MQ=255)

                                aGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc                                                                                                      >  2:1294943/1‑140 (MQ=255)

                                 gTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATAccc                                                                                                     >  2:221840/1‑140 (MQ=255)

                                  tCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCg                                                                                                    >  1:1217565/1‑140 (MQ=255)

                                  tCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCg                                                                                                    >  2:1283643/1‑140 (MQ=255)

                                   ccAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGt                                                                                                   <  1:870009/140‑1 (MQ=255)

                                     aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCa                                                                                                 <  1:832929/140‑1 (MQ=255)

                                     aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCa                                                                                                 <  1:507505/140‑1 (MQ=255)

                                     aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCa                                                                                                 <  1:1144532/140‑1 (MQ=255)

                                      gTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAg                                                                                                <  1:599455/140‑1 (MQ=255)

                                            ggCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTa                                                                                          >  2:152817/1‑140 (MQ=255)

                                            ggCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTa                                                                                          >  1:514552/1‑140 (MQ=255)

                                              ccTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAAt                                                                                        >  1:1011456/1‑140 (MQ=255)

                                                    tATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACggg                                                                                  <  2:1217565/140‑1 (MQ=255)

                                                     aTCCACTGTTTGCTCAGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGc                                                                                 <  2:840846/140‑1 (MQ=255)

                                                        cACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGtt                                                                              <  1:1184355/140‑1 (MQ=255)

                                                           tGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTca                                                                           >  1:974386/1‑140 (MQ=255)

                                                           tGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTca                                                                           <  2:913914/140‑1 (MQ=255)

                                                            gTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcac                                                                          >  1:281985/1‑140 (MQ=255)

                                                            gTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcac                                                                          >  2:1147465/1‑140 (MQ=255)

                                                             tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcaca                                                                         >  1:407104/1‑140 (MQ=255)

                                                               tGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTACTGACGGGCGTTTcactca                                                                       >  2:748827/1‑140 (MQ=255)

                                                                   ccGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGAt                                                                   <  1:924631/140‑1 (MQ=255)

                                                                     gCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTc                                                                 >  1:1138881/1‑140 (MQ=255)

                                                                     gCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTc                                                                 >  2:854797/1‑140 (MQ=255)

                                                                      ccTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTcc                                                                <  1:1170527/140‑1 (MQ=255)

                                                                      ccTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTcc                                                                <  1:1283643/140‑1 (MQ=255)

                                                                           gATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCg                                                           <  2:974386/140‑1 (MQ=255)

                                                                                     tGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCggg                                                 <  2:974038/140‑1 (MQ=255)

                                                                                          cctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGtt                                            >  2:6631/1‑139 (MQ=255)

                                                                                            tccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTAtt                                          >  1:532019/1‑140 (MQ=255)

                                                                                              cATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTAtttt                                        >  1:1160/1‑140 (MQ=255)

                                                                                                tCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGc                                      >  2:614754/1‑140 (MQ=255)

                                                                                                 cAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCt                                     >  2:917743/1‑140 (MQ=255)

                                                                                                   aaaaTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTgg                                   <  1:221840/140‑1 (MQ=255)

                                                                                                     aaTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCg                                 <  1:897322/140‑1 (MQ=255)

                                                                                                       tatGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGt                               <  2:1138881/140‑1 (MQ=255)

                                                                                                       aatGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGt                               <  2:1160/139‑1 (MQ=255)

                                                                                                         tGGACATGGGCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGttt                             <  1:1147465/140‑1 (MQ=255)

                                                                                                                gggCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAt                      >  1:1114002/1‑140 (MQ=255)

                                                                                                                 ggCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtg                     >  1:335466/1‑140 (MQ=255)

                                                                                                                 ggCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtg                     >  2:1101339/1‑140 (MQ=255)

                                                                                                                  gCTTTCGCAAGCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGG‑‑GGTATTTTGCTGGCGGTTTCCGTCAtgttg                  <  1:152817/140‑1 (MQ=255)

                                                                                                                            gCCCGGGAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCaa          >  2:1125348/1‑140 (MQ=255)

                                                                                                                                 ggAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGgcg     <  2:335466/140‑1 (MQ=255)

                                                                                                                                 ggAGCAGATTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGgcg     <  1:1101339/140‑1 (MQ=255)

                                                                                                                                   agcagaTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCa   <  1:160019/140‑1 (MQ=255)

                                                                                                                                   agcagaTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCa   <  2:1123751/140‑1 (MQ=255)

                                                                                                                                    gcagaTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAg  >  1:449632/1‑140 (MQ=255)

GGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAG  >  NC_000913/4142391‑4142662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A