Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F48 I1 R1	56	123.4	4244010	99.1%	4205813	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,062,257	C→A	L470I (CTT→ATT)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,062,257	0	C	A	100.0%	364.5 / NA	102	L470I (CTT→ATT)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (44/58); total (44/58)

Only 100 of 109 total aligned reads displayed.

AGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGC  >  NC_000913/3062121‑3062392

aGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTg                                                                                                                                      >  2:1238277/1‑140 (MQ=255)

 gCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTga                                                                                                                                     <  2:1609696/140‑1 (MQ=255)

 gCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTga                                                                                                                                     <  1:1528244/140‑1 (MQ=255)

  cGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTgag                                                                                                                                    <  2:505789/140‑1 (MQ=255)

   gATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAACCCGATGTAATTgaga                                                                                                                                   <  1:1544586/140‑1 (MQ=255)

   gATCGAAGCAGGTATGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTgaga                                                                                                                                   <  2:455245/140‑1 (MQ=255)

     tCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATc                                                                                                                                 <  1:752683/140‑1 (MQ=255)

     tCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATc                                                                                                                                 <  2:1550763/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGGTGTAATTGAGATCGa                                                                                                                               <  1:509095/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  1:1010030/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  2:1038973/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  1:183074/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  1:1571098/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  2:1749927/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  2:1958969/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  1:1073643/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  1:1125125/140‑1 (MQ=255)

       gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGa                                                                                                                               <  2:234055/140‑1 (MQ=255)

         aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACa                                                                                                                             <  1:498222/140‑1 (MQ=255)

         aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACa                                                                                                                             <  1:1176922/140‑1 (MQ=255)

         aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACa                                                                                                                             <  2:1611018/140‑1 (MQ=255)

         aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACa                                                                                                                             <  2:1656084/140‑1 (MQ=255)

         aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACa                                                                                                                             <  2:243400/140‑1 (MQ=255)

            aGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACg                                                                                                                          <  1:1023329/140‑1 (MQ=255)

              gTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTg                                                                                                                        <  1:1298326/140‑1 (MQ=255)

              gTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTg                                                                                                                        <  1:1494025/140‑1 (MQ=255)

              gTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTg                                                                                                                        <  1:1884871/140‑1 (MQ=255)

              gTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTg                                                                                                                        <  2:1045263/140‑1 (MQ=255)

              gTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTg                                                                                                                        <  2:1784441/140‑1 (MQ=255)

               tCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACGAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGa                                                                                                                       <  1:2063530/140‑1 (MQ=255)

               tCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGGCAACGTGa                                                                                                                       <  1:739703/140‑1 (MQ=255)

               tCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACTTGa                                                                                                                       >  1:1317899/1‑140 (MQ=255)

                cTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACACCGTGAt                                                                                                                      <  2:850270/140‑1 (MQ=255)

                cTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGAt                                                                                                                      <  1:1089558/140‑1 (MQ=255)

                   ccAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGt                                                                                                                   <  1:921158/140‑1 (MQ=255)

                       aaCGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGt                                                                                                               <  2:1317899/140‑1 (MQ=255)

                         cGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTaa                                                                                                             <  1:1911167/140‑1 (MQ=255)

                             tGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAg                                                                                                         >  2:469447/1‑140 (MQ=255)

                                  gAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAAt                                                                                                    <  1:748917/140‑1 (MQ=255)

                                        gCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTc                                                                                              <  2:1479093/140‑1 (MQ=255)

                                          cTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCgt                                                                                            >  2:190375/1‑139 (MQ=255)

                                          cTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGc                                                                                            <  1:1786853/140‑1 (MQ=255)

                                            cAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTg                                                                                          <  2:1152255/140‑1 (MQ=255)

                                             aGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTgg                                                                                         >  2:155044/1‑140 (MQ=255)

                                             aGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTgg                                                                                         >  2:207824/1‑140 (MQ=255)

                                               cgcgcgAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGaa                                                                                       <  2:1938018/140‑1 (MQ=255)

                                                 cgcgAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACg                                                                                     >  1:375584/1‑140 (MQ=255)

                                                  gcgAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGc                                                                                    >  2:2114297/1‑140 (MQ=255)

                                                   cgAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCa                                                                                   <  1:43577/140‑1 (MQ=255)

                                                         gTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTcgcg                                                                             <  2:1688365/140‑1 (MQ=255)

                                                          tCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCgcgc                                                                            <  1:864451/140‑1 (MQ=255)

                                                          tCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCgcgc                                                                            <  1:2114297/140‑1 (MQ=255)

                                                          tCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCgcgc                                                                            <  1:1238277/140‑1 (MQ=255)

                                                          tCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCgcgc                                                                            <  1:1029348/140‑1 (MQ=255)

                                                           cGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGcc                                                                           <  1:98824/140‑1 (MQ=255)

                                                              tGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCAcc                                                                        <  1:155044/140‑1 (MQ=255)

                                                                  cGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtg                                                                    >  2:544836/1‑140 (MQ=255)

                                                                    aCCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgat                                                                  >  1:521554/1‑140 (MQ=255)

                                                                    aCCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgat                                                                  >  2:1562207/1‑140 (MQ=255)

                                                                    aCCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgat                                                                  >  1:17635/1‑140 (MQ=255)

                                                                    aCCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgat                                                                  >  1:1989962/1‑140 (MQ=255)

                                                                       aGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTgatgat                                                               <  1:43162/140‑1 (MQ=255)

                                                                        gggCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGatgatg                                                              >  2:1777962/1‑140 (MQ=255)

                                                                        gggCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGatgatg                                                              >  1:1575644/1‑140 (MQ=255)

                                                                               cGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGt                                                       >  2:905520/1‑140 (MQ=255)

                                                                               cGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGt                                                       >  2:750461/1‑140 (MQ=255)

                                                                                gtgtCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTa                                                      >  2:2075965/1‑140 (MQ=255)

                                                                                   tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAAcc                                                   >  1:1451043/1‑140 (MQ=255)

                                                                                   tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAAcc                                                   >  2:1019755/1‑140 (MQ=255)

                                                                                   tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAAcc                                                   >  1:1005842/1‑140 (MQ=255)

                                                                                     aTCGTTGGTGTAAACAAGTAAAAACTGGATCACGAAGACAAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTCCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAAccgc                                                 <  2:1989962/140‑1 (MQ=255)

                                                                                         ttGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACcgccgct                                             >  2:183873/1‑139 (MQ=255)

                                                                                           ggTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGtt                                           >  2:693305/1‑140 (MQ=255)

                                                                                              gtCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTATTTGAGATCGACACCGTGAGGGTGCGTACCGAGCAATTTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                        <  2:1797063/140‑1 (MQ=255)

                                                                                              atCAACAAGTACAAACTGGATCACGAAGACGAACCCGATGTAATTGAGATCGACAACGTGATGGTGCGAAACGAGAAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                        <  2:2044351/139‑1 (MQ=255)

                                                                                               aaaaaaaGTACAAACGGGATCACGAAGACGAAACCAATGTATTTGAGATCACCACCGTGTGGGTGCGTAACGAGAAATTTGCTTCGCGGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                                       <  2:1005842/135‑1 (MQ=255)

                                                                                                  acaaGTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGcc                                    >  2:1131900/1‑140 (MQ=255)

                                                                                                      gTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGa                                >  1:892784/1‑140 (MQ=255)

                                                                                                      gTACAAACTGGATCACGAAGACGAAACCGATGTAATTGAAATCGACAACGTGATTGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGa                                >  2:527149/1‑140 (MQ=255)

                                                                                                         cAAACTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTc                             >  2:414797/1‑140 (MQ=255)

                                                                                                            aCTGGATCACGAAGACGAAACCGTTGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcg                          <  1:2037317/140‑1 (MQ=255)

                                                                                                            aCTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcg                          >  2:1541465/1‑140 (MQ=255)

                                                                                                             cTGGATCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc                         >  2:1601764/1‑140 (MQ=255)

                                                                                                               gaaTCACGAAGACGAAACCGTTGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGAACCGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgccg                       <  2:203499/138‑1 (MQ=255)

                                                                                                                 aTCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGca                     <  1:469447/140‑1 (MQ=255)

                                                                                                                 aTCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGca                     >  1:327272/1‑140 (MQ=255)

                                                                                                                 aTCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGca                     >  1:221757/1‑140 (MQ=255)

                                                                                                                 aTCACGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGca                     >  1:1037999/1‑140 (MQ=255)

                                                                                                                     cGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGc                 >  2:172554/1‑140 (MQ=255)

                                                                                                                     cGAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGc                 >  2:1326315/1‑140 (MQ=255)

                                                                                                                      gAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCa                <  1:750461/140‑1 (MQ=255)

                                                                                                                      gAAGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCa                >  2:1377786/1‑140 (MQ=255)

                                                                                                                        aGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATa              >  1:349410/1‑140 (MQ=255)

                                                                                                                        aGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATa              >  1:1127500/1‑140 (MQ=255)

                                                                                                                        aGACGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATa              >  1:176980/1‑140 (MQ=255)

                                                                                                                          aCGAAACCGATGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAAc            >  1:1663022/1‑140 (MQ=255)

                                                                                                                             aacccGATGTAATTGAAATCGACAACGTGATGGGGGGAACCGAGAAAATGGCTCCGCGGGAACGCTTTCGCGCCCCCCGTGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGaa         <  2:890882/137‑1 (MQ=255)

                                                                                                                                    tGTAATTGAGATCGACAGCGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCCCAGCATAACGAAAACctgc  >  1:304386/1‑140 (MQ=255)

                                                                                                                                    tGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACctgc  >  1:1211680/1‑140 (MQ=255)

                                                                                                                                    tGTAATTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACctgc  >  1:1481192/1‑140 (MQ=255)

AGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGGCAAGCGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGC  >  NC_000913/3062121‑3062392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A