Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F48 I1 R1 56 123.4 4244010 99.1% 4205813 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,394,408 G→T A115S (GCG→TCG)  nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,394,4080GT98.9% 321.6 / NA 93A115S (GCG→TCG) nnrNAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (31/61);  minor base A (0/1);  total (31/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCTAATTCCCA  >  NC_000913/4394272‑4394544
                                                                                                                                        |                                                                                                                                        
tGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCTCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCgg                                                                                                                                       <  1:454490/140‑1 (MQ=255)
tGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCgg                                                                                                                                       <  2:1121651/140‑1 (MQ=255)
aggTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCgg                                                                                                                                       <  2:197454/139‑1 (MQ=255)
  gTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCgggg                                                                                                                                     >  1:2121104/1‑139 (MQ=255)
  gTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGt                                                                                                                                     >  1:117665/1‑140 (MQ=255)
  gTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGt                                                                                                                                     <  2:1569536/140‑1 (MQ=255)
  gTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGt                                                                                                                                     <  2:27894/140‑1 (MQ=255)
     ataaCGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGc                                                                                                                                  <  2:102925/140‑1 (MQ=255)
     ataaCGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGc                                                                                                                                  <  1:1571557/140‑1 (MQ=255)
     ataaCGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGc                                                                                                                                  <  1:1550054/140‑1 (MQ=255)
      taaCGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCg                                                                                                                                 >  1:1644811/1‑140 (MQ=255)
       aaCGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGCTGTTGGCCCAGGAGAGCGCCAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGCTAAACTCGGGTGGCgg                                                                                                                                >  2:1878544/1‑139 (MQ=255)
          ggcggcGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGAt                                                                                                                             >  2:1052582/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATc                                                                                                                            >  2:1151647/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATc                                                                                                                            >  2:973283/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATc                                                                                                                            >  1:30809/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATc                                                                                                                            >  1:1610711/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATc                                                                                                                            >  1:1092972/1‑140 (MQ=255)
           gcggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGGGGCGAGATc                                                                                                                            >  1:151120/1‑140 (MQ=255)
            cggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATcc                                                                                                                           <  2:117665/140‑1 (MQ=255)
            cggcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATcc                                                                                                                           >  1:1636290/1‑140 (MQ=255)
              gcgATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCAt                                                                                                                         <  1:1764379/140‑1 (MQ=255)
                 aTGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCt                                                                                                                      <  1:393707/140‑1 (MQ=255)
                 aTGGCTACGTGGTCGCGCGACTGGCCAAAGCGGGCGGCATTGAGGGCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGGAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGGTCCATGCt                                                                                                                      >  2:1172300/1‑140 (MQ=255)
                  tGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCtt                                                                                                                     <  2:1286085/140‑1 (MQ=255)
                   ggCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCTGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTc                                                                                                                    <  2:1183736/140‑1 (MQ=255)
                       aCGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAat                                                                                                                <  2:181119/140‑1 (MQ=255)
                       aCGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAat                                                                                                                <  2:626145/140‑1 (MQ=255)
                        cGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAata                                                                                                               <  1:1146669/140‑1 (MQ=255)
                        cGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAata                                                                                                               <  1:1076811/140‑1 (MQ=255)
                            gTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGt                                                                                                           <  1:1687360/140‑1 (MQ=255)
                             tCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTc                                                                                                          <  1:2105676/140‑1 (MQ=255)
                              cgcgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCt                                                                                                         >  2:1038319/1‑140 (MQ=255)
                              cgcgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCt                                                                                                         >  1:963073/1‑140 (MQ=255)
                              cgcgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCt                                                                                                         >  1:1383625/1‑140 (MQ=255)
                              cgcgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCt                                                                                                         >  2:955761/1‑140 (MQ=255)
                              cgcgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCt                                                                                                         >  2:814463/1‑140 (MQ=255)
                                cgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTgg                                                                                                       >  1:1407373/1‑140 (MQ=255)
                                cgcgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTgg                                                                                                       <  2:1636290/140‑1 (MQ=255)
                                  cgACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGcc                                                                                                     <  1:1659024/140‑1 (MQ=255)
                                     cTGGCCAAGGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGa                                                                                                  <  2:2121104/140‑1 (MQ=255)
                                     cTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACTGTTGCAGGATGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGa                                                                                                  <  2:1127582/140‑1 (MQ=255)
                                     cTGACCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGa                                                                                                  <  2:427611/140‑1 (MQ=255)
                                       ggCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAAt                                                                                                <  2:637795/140‑1 (MQ=255)
                                           aaaGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGt                                                                                            <  1:1668153/140‑1 (MQ=255)
                                           aaaGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGAGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGt                                                                                            <  1:1237825/140‑1 (MQ=255)
                                             aGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAg                                                                                          <  1:973283/140‑1 (MQ=255)
                                                  tCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTg                                                                                     >  1:492281/1‑140 (MQ=255)
                                                         tGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGAttgttg                                                                              >  1:1553059/1‑140 (MQ=255)
                                                            ggTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATg                                                                           <  1:955761/140‑1 (MQ=255)
                                                            ggTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATg                                                                           <  1:722243/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  2:1022459/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  1:1061411/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  2:1644811/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  1:1868411/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  1:1928631/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  2:933648/140‑1 (MQ=255)
                                                              tCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATgcg                                                                         <  1:2028279/140‑1 (MQ=255)
                                                                aCGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCgct                                                                       >  1:791319/1‑140 (MQ=255)
                                                                 cGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCgctg                                                                      <  1:1421135/140‑1 (MQ=255)
                                                                 cGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCgctg                                                                      <  1:346369/140‑1 (MQ=255)
                                                                   ttgttgGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCgctgct                                                                    <  2:560976/140‑1 (MQ=255)
                                                                     gttgGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCg                                                                  >  1:1534897/1‑140 (MQ=255)
                                                                       tgGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCA‑GGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTa                                                               >  2:1065727/1‑140 (MQ=255)
                                                                        ggCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACACGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTa                                                               <  1:300936/140‑1 (MQ=255)
                                                                              ggtgagCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGtt                                                         >  1:2022618/4‑140 (MQ=255)
                                                                               gagaGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGttt                                                        <  2:2089189/140‑1 (MQ=255)
                                                                                    cGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGc                                                   >  2:1282422/1‑140 (MQ=255)
                                                                                    cGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGc                                                   >  1:631336/1‑140 (MQ=255)
                                                                                     gACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCa                                                  <  2:1553059/140‑1 (MQ=255)
                                                                                         aaCCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAgcg                                              >  1:1530636/1‑140 (MQ=255)
                                                                                             gTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGcccc                                          >  1:324694/1‑140 (MQ=255)
                                                                                             gTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGcccc                                          <  1:788703/140‑1 (MQ=255)
                                                                                                 ccGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGa                                      <  2:1092972/140‑1 (MQ=255)
                                                                                                 ccGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGa                                      <  2:492281/140‑1 (MQ=255)
                                                                                                 ccGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGa                                      <  2:1407373/140‑1 (MQ=255)
                                                                                                   ggAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAAt                                    <  2:1913154/140‑1 (MQ=255)
                                                                                                     aaGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATcc                                  >  2:1005575/1‑140 (MQ=255)
                                                                                                      agagGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCa                                 <  2:1821561/140‑1 (MQ=255)
                                                                                                        agGCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCAtt                               <  1:1090488/140‑1 (MQ=255)
                                                                                                         ggCCGCGCTGGCACGCGAAGCA‑GGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAg                             <  1:1065727/140‑1 (MQ=255)
                                                                                                          gCCGCGCTGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAg                             <  1:1038319/140‑1 (MQ=255)
                                                                                                                 tGGCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTa                      <  2:1383625/140‑1 (MQ=255)
                                                                                                                  ggCACGCGAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTaa                     <  2:1530636/140‑1 (MQ=255)
                                                                                                                       gcgAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGAc                <  2:523244/140‑1 (MQ=255)
                                                                                                                       gcgAAGCATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGAc                <  2:684675/140‑1 (MQ=255)
                                                                                                                             cATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGTTTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  1:814463/140‑1 (MQ=255)
                                                                                                                             cATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:151120/140‑1 (MQ=255)
                                                                                                                             cATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:2022618/140‑1 (MQ=255)
                                                                                                                             cATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:30809/140‑1 (MQ=255)
                                                                                                                             cATGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:1824250/140‑1 (MQ=255)
                                                                                                                             aaTGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:324694/139‑1 (MQ=255)
                                                                                                                             aaTGGTTAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCt          <  2:584125/139‑1 (MQ=255)
                                                                                                                                  ttAAACTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCTAATTc     <  1:1005575/140‑1 (MQ=255)
                                                                                                                                     aaCTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCTAATTccct  >  2:1585865/1‑139 (MQ=255)
                                                                                                                                     aaCTCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCTAATTCCCa  >  1:1568586/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGTAGATCTGATTGTTGATGCGCTGCTCGGTACCGGTTTGCGGCAAGCGCCCCGCGAATCCATTAGCCAGTTAATCGACCACGCTAATTCCCA  >  NC_000913/4394272‑4394544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A