Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,263	C→A	P167Q (CCG→CAG)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,263	0	C	A	100.0%	138.1 / NA	39	P167Q (CCG→CAG)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (25/14); total (25/14)

TATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATT  >  NC_000913/3009126‑3009396

tATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAgg                                                                                                                                     >  1:537391/1‑140 (MQ=255)

     tttATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAAttt                                                                                                                                <  2:592569/140‑1 (MQ=255)

     tttATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAAttt                                                                                                                                >  1:592569/1‑140 (MQ=255)

         tGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTgg                                                                                                                            >  1:602979/1‑140 (MQ=255)

          gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGt                                                                                                                           <  2:617400/140‑1 (MQ=255)

          gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGt                                                                                                                           <  2:537391/140‑1 (MQ=255)

              gTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGt                                                                                                                       >  2:210935/1‑140 (MQ=255)

              gTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGt                                                                                                                       >  1:42318/1‑140 (MQ=255)

                            gACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGa                                                                                                         <  2:42318/140‑1 (MQ=255)

                             aCCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGAc                                                                                                        >  2:387139/1‑140 (MQ=255)

                               cTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTg                                                                                                      >  1:837969/1‑140 (MQ=255)

                                tCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGc                                                                                                     >  2:750431/1‑140 (MQ=255)

                                tCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGc                                                                                                     <  2:774651/140‑1 (MQ=255)

                                  ggTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCa                                                                                                   >  2:218093/1‑140 (MQ=255)

                                                    aCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                                                                 <  2:472426/140‑1 (MQ=255)

                                                       ctgctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCgg                                                                              <  1:387139/140‑1 (MQ=255)

                                                         gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                                                            >  2:812031/1‑140 (MQ=255)

                                                         gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                                                            >  1:367487/1‑140 (MQ=255)

                                                         gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                                                            <  1:316388/140‑1 (MQ=255)

                                                             gTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTAt                                                                        >  1:194583/1‑140 (MQ=255)

                                                               tACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATgg                                                                      >  1:680583/1‑140 (MQ=255)

                                                                     tACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTc                                                                >  1:755510/1‑140 (MQ=255)

                                                                          tGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTAtt                                                           >  1:274219/1‑140 (MQ=255)

                                                                            cAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGa                                                         <  2:194583/140‑1 (MQ=255)

                                                                               gaagaaGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGt                                                      >  2:743012/1‑140 (MQ=255)

                                                                               gaagaaGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGt                                                      >  2:386876/1‑140 (MQ=255)

                                                                                aagaagACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGtt                                                     <  2:602979/140‑1 (MQ=255)

                                                                                aagaagACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGtt                                                     <  2:680583/140‑1 (MQ=255)

                                                                                          cGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTa                                           >  2:285403/1‑140 (MQ=255)

                                                                                                 cTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCa                                    <  1:750431/140‑1 (MQ=255)

                                                                                                         gCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTg                            >  2:90506/1‑140 (MQ=255)

                                                                                                         gCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTg                            >  1:17990/1‑140 (MQ=255)

                                                                                                         gCAGTACATTATTGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTg                            >  2:351614/1‑140 (MQ=255)

                                                                                                                   attGAACAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAAcg                  <  2:766173/140‑1 (MQ=255)

                                                                                                                         cAATCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGa            >  1:274987/1‑140 (MQ=255)

                                                                                                                            tCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATaa         >  2:417994/1‑140 (MQ=255)

                                                                                                                            tCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATaa         >  1:415123/1‑140 (MQ=255)

                                                                                                                            tCCGGCATTCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATaa         >  2:817675/1‑140 (MQ=255)

                                                                                                                                   ttCGCCAGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCAtt  <  2:274987/140‑1 (MQ=255)

TATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATT  >  NC_000913/3009126‑3009396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A