Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,057,844	G→T	G189G (GGC→GGA)	serA ←	phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,057,844	0	G	T	100.0%	150.5 / NA	43	G189G (GGC→GGA)	serA	phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/21); total (22/21)

CAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTGCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCCGCGCGCTTCAAAAGAACC  >  NC_000913/3057709‑3057978

cagcGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCa                                                                                                                                    >  2:761580/1‑140 (MQ=255)

   cGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCg                                                                                                                                 >  1:467973/1‑140 (MQ=255)

   cGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCg                                                                                                                                 >  2:194147/1‑140 (MQ=255)

        cGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGt                                                                                                                            <  1:748162/140‑1 (MQ=255)

          ggCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACAACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGttt                                                                                                                          <  2:572748/140‑1 (MQ=255)

                  tAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCaa                                                                                                                  <  1:40277/140‑1 (MQ=255)

                   aGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAat                                                                                                                 <  2:820940/140‑1 (MQ=255)

                     tGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAatat                                                                                                               >  2:85600/1‑140 (MQ=255)

                         aTTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATa                                                                                                           <  1:1077/140‑1 (MQ=255)

                         aTTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATa                                                                                                           >  2:579026/1‑140 (MQ=255)

                               ttcGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTa                                                                                                     <  2:589948/140‑1 (MQ=255)

                                    gcCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATaaaa                                                                                                        <  2:782127/132‑1 (MQ=255)

                                    gcCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATaaaa                                                                                                        >  1:782127/1‑132 (MQ=255)

                                    gcCCATCATATTTTTGGTAGACGGATTCTTTGGTGCATGCAGACTCACGGCATCGCTCATATTCAGAAGGTCAGAAGGATGCTGTACCTGAGTGGCGTTTACCAGCGGCAGTTTATTTTCAATATCATAGAAGTAAacat                                                                                                <  2:363709/140‑1 (MQ=255)

                                                         cGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCa                                                                           <  1:654514/140‑1 (MQ=255)

                                                         cGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCa                                                                           <  1:85600/140‑1 (MQ=255)

                                                         cGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCa                                                                           >  1:416104/1‑140 (MQ=255)

                                                         cGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCa                                                                           <  1:635105/140‑1 (MQ=255)

                                                           gATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGa                                                                         <  2:467973/140‑1 (MQ=255)

                                                              tctcTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATg                                                                      <  1:631309/140‑1 (MQ=255)

                                                                tctGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGcc                                                                    <  1:755014/140‑1 (MQ=255)

                                                                       aaTGCAGACTCACCACATCGCTCATATGCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGGGTATCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACACTCCCAGCGATTCAGGCAGAATACCCAATGGc                                                             <  2:639215/139‑1 (MQ=255)

                                                                        aTGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGct                                                            >  2:227712/1‑139 (MQ=255)

                                                                         tGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGt                                                           >  1:788138/1‑140 (MQ=255)

                                                                            aGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTAcc                                                        >  2:739586/1‑140 (MQ=255)

                                                                              aCTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCaa                                                      >  1:514076/1‑140 (MQ=255)

                                                                                  accacATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAatatt                                                  >  2:57363/1‑139 (MQ=255)

                                                                                  accacATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATg                                                  >  1:167707/1‑140 (MQ=255)

                                                                                    cacaTCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGAc                                                >  1:456207/1‑140 (MQ=255)

                                                                                     acaTCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGAcc                                               <  1:788141/140‑1 (MQ=255)

                                                                                          gCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGc                                          <  2:554070/140‑1 (MQ=255)

                                                                                            tCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCg                                        >  1:663808/1‑140 (MQ=255)

                                                                                             cATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCga                                       <  2:456207/140‑1 (MQ=255)

                                                                                              atatTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACTAATATGACCGTAGCCgat                                      <  1:579026/140‑1 (MQ=255)

                                                                                                      cagGTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCa                              >  2:451405/1‑140 (MQ=255)

                                                                                                         gTCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATTATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCt                           >  2:234887/1‑140 (MQ=255)

                                                                                                          tCAGAAAGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCATAGCCGATGATACCCAGCtt                          >  2:244104/1‑140 (MQ=255)

                                                                                                              aaaGATGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTTTTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATTACCGTAGCCGATGATACCCAGCTTTTTg                      >  2:633774/1‑140 (MQ=255)

                                                                                                                   tGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCcgcg                 <  2:416104/140‑1 (MQ=255)

                                                                                                                   tGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCcgcg                 <  1:194147/140‑1 (MQ=255)

                                                                                                                   tGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCcgcg                 >  1:593267/1‑140 (MQ=255)

                                                                                                                   tGCTGTACCTGAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCATAGCCGATGATACCCAGCTTTTTGCcgcg                 <  1:244104/140‑1 (MQ=255)

                                                                                                                             gAGTGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCCGCGCGCTTCaaaa       >  1:148272/1‑140 (MQ=255)

                                                                                                                                tGGCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCCGCGCGCTTCAAAAGaa    >  2:171086/1‑140 (MQ=255)

                                                                                                                                  gCGTTTCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCCGCGCGCTTCAAAAGAAcc  >  2:403421/1‑140 (MQ=255)

CAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGGTACATGCAGACTCACCACATCGCTCATATTCAGCAGGTCAGAAAGATGCTGTACCTGAGTGGCGTTGCCCAGCGGCAGTTTATTTTCAATATCATAAAAGTAAACATACATTCCCAGCGATTCAGCCAGAATGCCCAATTGCGTACCAATATGACCGTAGCCGATGATACCCAGCTTTTTGCCGCGCGCTTCAAAAGAACC  >  NC_000913/3057709‑3057978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A