Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,215,442	C→A	R11L (CGG→CTG)	mug ←	stationary phase mismatch/uracil DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,215,442	0	C	A	100.0%	172.6 / NA	48	R11L (CGG→CTG)	mug	stationary phase mismatch/uracil DNA glycosylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/34); total (14/34)

GCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGT  >  NC_000913/3215307‑3215576

gCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTaa                                                                                                                                    >  2:16197/1‑140 (MQ=255)

     ctGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTg                                                                                                                               <  1:376114/140‑1 (MQ=255)

      tGCGGCTTCAAGTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTgg                                                                                                                              >  2:671940/1‑140 (MQ=255)

             tCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCaaa                                                                                                                       <  1:730068/140‑1 (MQ=255)

             tCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCaaa                                                                                                                       <  2:455964/140‑1 (MQ=255)

              cAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCaaaa                                                                                                                      <  1:554274/140‑1 (MQ=255)

                  tGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATc                                                                                                                  <  2:842618/140‑1 (MQ=255)

                  tGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATc                                                                                                                  <  2:649667/140‑1 (MQ=255)

                    aCGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCt                                                                                                                <  2:599610/140‑1 (MQ=255)

                    aCGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCt                                                                                                                <  2:276238/140‑1 (MQ=255)

                      ggtcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCa                                                                                                              <  1:799865/140‑1 (MQ=255)

                        tcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAAc                                                                                                            <  2:265694/140‑1 (MQ=255)

                        tcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAAc                                                                                                            <  2:70839/140‑1 (MQ=255)

                        tcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAAc                                                                                                            <  1:671940/140‑1 (MQ=255)

                         aggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAAcc                                                                                                           <  2:687504/139‑1 (MQ=255)

                           gtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCat                                                                                                         <  1:21378/140‑1 (MQ=255)

                                cccGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTa                                                                                                    <  1:678074/140‑1 (MQ=255)

                                  cGGCCTGATATATCCCCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGAGTTGATACCGCAACACCCGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACt                                                                                                  <  1:805810/140‑1 (MQ=255)

                                  cGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACt                                                                                                  <  1:151754/140‑1 (MQ=255)

                                               cACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATc                                                                                     >  2:265776/1‑140 (MQ=255)

                                                  cTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCAcc                                                                                  >  1:265447/1‑140 (MQ=255)

                                                   ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACt                                                                                                  >  1:597014/1‑123 (MQ=255)

                                                   ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACt                                                                                                  <  2:597014/123‑1 (MQ=255)

                                                   ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                 >  2:130429/1‑140 (MQ=255)

                                                    tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAg                                                                                >  2:270761/1‑140 (MQ=255)

                                                    tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAg                                                                                >  1:90481/1‑140 (MQ=255)

                                                     ccAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAgg                                                                               <  1:255332/140‑1 (MQ=255)

                                                       aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                             <  2:265447/140‑1 (MQ=255)

                                                       aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                             <  2:387550/140‑1 (MQ=255)

                                                       aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                             <  1:265776/140‑1 (MQ=255)

                                                       aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                             <  2:620598/140‑1 (MQ=255)

                                                               tttGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATaa                                                                     >  2:709677/1‑140 (MQ=255)

                                                                    cGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATGCCGCAAAACACGACCAGTATCCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGAt                                                                <  1:625232/140‑1 (MQ=255)

                                                                         gAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGaaa                                                           <  2:221911/140‑1 (MQ=255)

                                                                              aaGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACa                                                      <  2:818268/140‑1 (MQ=255)

                                                                                      aCCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGTAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTtat                                              >  1:718923/1‑140 (MQ=255)

                                                                                       acAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTtata                                             <  2:657246/139‑1 (MQ=255)

                                                                                             ccGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGAAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAAca                                       >  2:280075/1‑140 (MQ=255)

                                                                                                     tGAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgc                               <  1:359618/140‑1 (MQ=255)

                                                                                                      gAAAGCCCAGGGTTGATACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgcg                              <  1:225618/140‑1 (MQ=255)

                                                                                                                     aTACCGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGAt               >  2:193457/1‑140 (MQ=255)

                                                                                                                         cGCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGG‑AAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGa          <  2:646054/140‑1 (MQ=255)

                                                                                                                          gCAAAACACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGa          >  2:124552/1‑140 (MQ=255)

                                                                                                                            aaaaCACGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGaca        <  1:16197/140‑1 (MQ=255)

                                                                                                                                cacGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGa    >  2:820615/1‑140 (MQ=255)

                                                                                                                                 acGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAg   <  2:59088/140‑1 (MQ=255)

                                                                                                                                 acGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAg   <  1:193457/140‑1 (MQ=255)

                                                                                                                                  cGACCAGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGt  <  1:827373/140‑1 (MQ=255)

GCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGT  >  NC_000913/3215307‑3215576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A