Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,377,264	G→T	L53I (CTC→ATC)	sspA ←	stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,377,264	0	G	T	100.0%	178.4 / NA	51	L53I (CTC→ATC)	sspA	stringent starvation protein A
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (24/27); total (24/27)

CACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGAAC  >  NC_000913/3377129‑3377398

cACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCa                                                                                                                                    <  2:758461/140‑1 (MQ=255)

      gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCCCAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGa                                                                                                                              >  1:628041/1‑140 (MQ=255)

      gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGa                                                                                                                              >  2:405193/1‑140 (MQ=255)

                 tAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATTATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCgg                                                                                                                   >  1:589185/1‑140 (MQ=255)

                 tAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCgg                                                                                                                   >  2:852022/1‑140 (MQ=255)

                  aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGa                                                                                                                  <  1:66032/140‑1 (MQ=255)

                  aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGa                                                                                                                  <  2:182800/140‑1 (MQ=255)

                  aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGa                                                                                                                  <  1:283890/140‑1 (MQ=255)

                      aGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGt                                                                                                              <  1:405193/140‑1 (MQ=255)

                        gCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTcc                                                                                                            <  2:685339/140‑1 (MQ=255)

                           tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCttt                                                                                                         >  1:742564/1‑140 (MQ=255)

                                       gCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg                                                                                             <  1:852022/140‑1 (MQ=255)

                                          ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtt                                                                                          >  2:11482/1‑139 (MQ=255)

                                             aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg                                                                                       <  2:344050/140‑1 (MQ=255)

                                             aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg                                                                                       <  2:308816/140‑1 (MQ=255)

                                             aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg                                                                                       <  1:389757/140‑1 (MQ=255)

                                                  tCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACt                                                                                  >  1:802505/1‑140 (MQ=255)

                                                   cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt                                                                                 >  1:272550/1‑140 (MQ=255)

                                                      ccAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTaca                                                                              <  2:272550/140‑1 (MQ=255)

                                                      ccAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTaca                                                                              >  2:136590/1‑140 (MQ=255)

                                                       cAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTacac                                                                             <  2:628041/140‑1 (MQ=255)

                                                        aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAcc                                                                            >  1:764106/1‑140 (MQ=255)

                                                          atatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCtt                                                                          >  1:714053/1‑140 (MQ=255)

                                                              tCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtctc                                                                      >  1:791073/1‑140 (MQ=255)

                                                                cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcag                                                                    >  1:155030/1‑140 (MQ=255)

                                                                cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcag                                                                    >  1:826015/1‑140 (MQ=255)

                                                                 catattatgCGAGATTCCCACAGGGTCAGCTCACGGTCCACCCGGGTCGGAACGCTCTGATTCGGGTTGGTGTCCATCAGATTCTGAGGCGGAATGTCCTTTTTCACGGGTTCGATCTCGAAACTTACACCTTTCTcagc                                                                   >  2:841485/7‑140 (MQ=255)

                                                                 aTAATGATGCGAGCTTCCCGCAGTGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACCCCTTCCTcagc                                                                   <  2:688010/140‑1 (MQ=255)

                                                                   aaTGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagcca                                                                 >  2:443469/1‑140 (MQ=255)

                                                                    atgatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagccag                                                                >  2:309300/1‑140 (MQ=255)

                                                                    atgatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagccag                                                                >  1:570199/1‑140 (MQ=255)

                                                                      gatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAACCTTACACCTTTCTCAGCCAGca                                                              <  2:791073/140‑1 (MQ=255)

                                                                      gatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagccagcc                                                              <  2:802505/140‑2 (MQ=255)

                                                                       atgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGcac                                                             >  2:617108/1‑140 (MQ=255)

                                                                        tgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGcaca                                                            >  1:627773/1‑140 (MQ=255)

                                                                        tgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGcaca                                                            >  1:434864/1‑140 (MQ=255)

                                                                                   cacaGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTg                                                 <  1:617108/140‑1 (MQ=255)

                                                                                     caGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGAt                                               <  1:443469/140‑1 (MQ=255)

                                                                                     caGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGAt                                               <  1:356809/140‑1 (MQ=255)

                                                                                       gggTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATgg                                             <  1:415041/140‑1 (MQ=255)

                                                                                           cAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtat                                         <  1:726904/140‑1 (MQ=255)

                                                                                            aGCTCACGATCCGCCAGGGTCGGAACGCTCTGATTCAGGTTGATGTCAATCAGATCATGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtata                                        <  2:18339/140‑1 (MQ=255)

                                                                                            aGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtata                                        >  1:773805/1‑140 (MQ=255)

                                                                                            aGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtata                                        >  1:773631/1‑140 (MQ=255)

                                                                                                  cGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTc                                  >  2:136452/1‑140 (MQ=255)

                                                                                                         ccaGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGa                           <  2:764106/140‑1 (MQ=255)

                                                                                                            gggTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCg                        <  2:714053/140‑1 (MQ=255)

                                                                                                                 ggAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGaaaa                   <  1:187414/140‑1 (MQ=255)

                                                                                                                  gAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAAc                  <  2:589185/140‑1 (MQ=255)

                                                                                                                  gAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAAc                  <  2:773805/140‑1 (MQ=255)

                                                                                                                    aCGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAg                <  2:570199/140‑1 (MQ=255)

                                                                                                                               cGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCg     <  1:309300/140‑1 (MQ=255)

                                                                                                                                 ggTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGaa   <  1:841485/140‑1 (MQ=255)

                                                                                                                                  gTTGATGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGAAc  >  1:740379/1‑140 (MQ=255)

CACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGAAC  >  NC_000913/3377129‑3377398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A