Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,616,203	C→A	R8S (CGC→AGC)	nikC →	Ni(2(+)) ABC transporter membrane subunit NikC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,616,203	0	C	A	100.0%	126.2 / NA	36	R8S (CGC→AGC)	nikC	Ni(2(+)) ABC transporter membrane subunit NikC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/14); total (22/14)

GATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCCGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGC  >  NC_000913/3616068‑3616330

gATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTg                                                                                                                             <  2:223777/140‑1 (MQ=255)

  tCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGt                                                                                                                           >  1:560381/1‑140 (MQ=255)

       tGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTa                                                                                                                      >  2:296991/1‑140 (MQ=255)

        gCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTAc                                                                                                                     >  1:627471/1‑140 (MQ=255)

           ttACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGcc                                                                                                                  <  1:217804/140‑1 (MQ=255)

            tACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCt                                                                                                                 >  2:776342/1‑140 (MQ=255)

            tACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCt                                                                                                                 >  2:283012/1‑140 (MQ=255)

              cGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTGCCTCTCTGCCAGCTGGTCGGTAGGCCTgg                                                                                                               <  2:809428/140‑1 (MQ=255)

                cTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGgcg                                                                                                             <  2:688215/140‑1 (MQ=255)

                 tgatgaTGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGgcgc                                                                                                            >  1:790310/1‑140 (MQ=255)

                   atgatgGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTg                                                                                                          >  2:119473/1‑140 (MQ=255)

                   atgatgGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTg                                                                                                          >  1:92521/1‑140 (MQ=255)

                   atgatgGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTg                                                                                                          >  1:150997/1‑140 (MQ=255)

                             tggtTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCg                                                                                                <  2:155233/140‑1 (MQ=255)

                                    tgtgGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCctgct                                                                                         >  1:729204/1‑140 (MQ=255)

                                    tgtgGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCctgct                                                                                         >  2:76509/1‑140 (MQ=255)

                                    tgtgGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCctgct                                                                                         <  2:560381/140‑1 (MQ=255)

                                                       gTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCAcc                                                                      >  2:518783/1‑140 (MQ=255)

                                                          gATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCAccagc                                                                   <  1:39059/140‑1 (MQ=255)

                                                          gATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCAccagc                                                                   <  1:76509/140‑1 (MQ=255)

                                                                     cgccgcGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCt                                                        >  2:203659/1‑140 (MQ=255)

                                                                      gccgcGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTg                                                       <  2:150997/140‑1 (MQ=255)

                                                                          cgcTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGt                                                   >  1:512373/1‑140 (MQ=255)

                                                                          cgcTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGt                                                   >  1:332814/1‑140 (MQ=255)

                                                                                    gcgcATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCcaca                                         >  2:534771/1‑140 (MQ=255)

                                                                                     cgcATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAg                                        >  2:27753/1‑140 (MQ=255)

                                                                                     cgcATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAg                                        >  1:573786/1‑140 (MQ=255)

                                                                                       cATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGc                                      <  1:119473/140‑1 (MQ=255)

                                                                                                    gAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCg                         >  1:45290/1‑140 (MQ=255)

                                                                                                           cgcACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGcc                  >  1:853924/1‑140 (MQ=255)

                                                                                                           cgcACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGcc                  >  2:795001/1‑140 (MQ=255)

                                                                                                             cACGCGTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTg                >  1:262291/1‑140 (MQ=255)

                                                                                                               cgcgTGAACTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCt              <  1:534771/140‑1 (MQ=255)

                                                                                                                       cTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCgg      <  1:644199/140‑1 (MQ=255)

                                                                                                                       cTTTTTCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCgg      <  2:512373/140‑1 (MQ=255)

                                                                                                                         ttttCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGAt    <  2:418294/140‑1 (MQ=255)

                                                                                                                           ttCCTCTCTTCCAGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATgc  <  2:729204/140‑1 (MQ=255)

GATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCTCTTCCCGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGC  >  NC_000913/3616068‑3616330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A