Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,041,319	C→A	G39W (GGG→TGG)	mobB ←	molybdopterin‑guanine dinucleotide biosynthesis adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,041,319	0	C	A	100.0%	137.0 / NA	39	G39W (GGG→TGG)	mobB	molybdopterin‑guanine dinucleotide biosynthesis adaptor protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/18); total (21/18)

TCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTA  >  NC_000913/4041183‑4041454

tCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATgg                                                                                                                                      <  2:537892/140‑1 (MQ=255)

 cTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGa                                                                                                                                     <  2:205644/140‑1 (MQ=255)

        ttCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATcc                                                                                                                              <  2:528150/140‑1 (MQ=255)

          ccGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCct                                                                                                                            >  1:358272/1‑140 (MQ=255)

              catcaAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTgg                                                                                                                        <  2:409112/140‑1 (MQ=255)

                  aaGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCa                                                                                                                    <  1:749389/140‑1 (MQ=255)

                  aaGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCa                                                                                                                    <  2:114085/140‑1 (MQ=255)

                  aaGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCa                                                                                                                    <  2:379507/140‑1 (MQ=255)

                             ttGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGa                                                                                                         <  1:555646/140‑1 (MQ=255)

                                          aCGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCaa                                                                                            <  2:475278/140‑1 (MQ=255)

                                                     tgCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGttt                                                                                 >  1:519173/1‑140 (MQ=255)

                                                        cgcgCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTc                                                                              <  2:668536/140‑1 (MQ=255)

                                                          cgcCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCg                                                                            >  1:383414/1‑140 (MQ=255)

                                                                                 cTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGc                                                     <  1:585949/140‑1 (MQ=255)

                                                                                    tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAg                                                  >  1:116402/1‑140 (MQ=255)

                                                                                    tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAg                                                  >  1:645850/1‑140 (MQ=255)

                                                                                    tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAg                                                  >  2:161449/1‑140 (MQ=255)

                                                                                       ttGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTaa                                               <  1:114237/140‑1 (MQ=255)

                                                                                              gCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTatc                                        >  1:793055/1‑140 (MQ=255)

                                                                                              gCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAG‑TTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTatca                                       >  2:796473/1‑140 (MQ=255)

                                                                                                   tCAACATCCATATCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGt                                   >  2:424741/1‑140 (MQ=255)

                                                                                                            atatCATGGTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGc                          >  1:177900/1‑140 (MQ=255)

                                                                                                                   ggTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCggg                   >  1:258054/1‑140 (MQ=255)

                                                                                                                    gTGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGc                  >  1:210922/1‑140 (MQ=255)

                                                                                                                     tGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCa                 >  1:371455/1‑140 (MQ=255)

                                                                                                                     tGCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCa                 <  1:710022/140‑1 (MQ=255)

                                                                                                                      gCGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGTCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCaa                >  2:286542/1‑140 (MQ=255)

                                                                                                                       cGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAg               >  2:431377/1‑140 (MQ=255)

                                                                                                                       cGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAg               <  2:359830/140‑1 (MQ=255)

                                                                                                                       cGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAg               <  2:358272/140‑1 (MQ=255)

                                                                                                                       cGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAg               <  1:103727/140‑1 (MQ=255)

                                                                                                                       cGTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAg               >  2:344220/1‑140 (MQ=255)

                                                                                                                        gTATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGc              >  1:36317/1‑140 (MQ=255)

                                                                                                                         tATGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGct             >  1:673135/1‑140 (MQ=255)

                                                                                                                          aTGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGctc            >  2:578037/1‑140 (MQ=255)

                                                                                                                          aTGCTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGctc            <  2:793737/140‑1 (MQ=255)

                                                                                                                             cTTAATCAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGctcctc         >  1:230689/1‑140 (MQ=255)

                                                                                                                                   cAGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGTCGAGTAACGAGATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGACgg   >  2:498610/1‑139 (MQ=255)

                                                                                                                                    aGCCATGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTa  <  2:454263/140‑1 (MQ=255)

TCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTA  >  NC_000913/4041183‑4041454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A