Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,146,727	C→A	A157E (GCA→GAA)	pflC →	putative pyruvate formate‑lyase 2 activating enzyme PflC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,727	0	C	A	100.0%	139.7 / NA	40	A157E (GCA→GAA)	pflC	putative pyruvate formate‑lyase 2 activating enzyme PflC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/19); total (21/19)

CATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTG  >  NC_000913/4146590‑4146860

aaTTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAAc                                                                                                                                     <  2:703497/139‑1 (MQ=255)

  ttttCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAAcca                                                                                                                                   <  2:589337/140‑1 (MQ=255)

    ttctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAAccagc                                                                                                                                 <  2:16985/140‑1 (MQ=255)

      ctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAAccagcca                                                                                                                               >  1:631739/1‑140 (MQ=255)

          cGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGCGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCaa                                                                                                                           >  1:176047/1‑140 (MQ=255)

                       gcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTg                                                                                                              <  2:153639/140‑1 (MQ=255)

                         ggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGc                                                                                                            >  1:503790/1‑140 (MQ=255)

                             tCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGaaa                                                                                                        <  2:464947/140‑1 (MQ=255)

                                  cTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATtgtg                                                                                                   <  1:641023/140‑1 (MQ=255)

                                                         gCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaa                                                                            >  1:477428/1‑140 (MQ=255)

                                                          cAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaa                                                                           >  2:18542/1‑140 (MQ=255)

                                                            ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaaaa                                                                         >  1:357045/1‑140 (MQ=255)

                                                            ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaaaa                                                                         >  2:136363/1‑140 (MQ=255)

                                                               caggTTTGCTACCCGTTGTTTCCGGC‑AATGCAGGCAGTGGGGTGTGTCGTGCGCCATTGAATCTGCCGGAGACGAACCAGCCAGGAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTa                                                                      <  2:377055/137‑1 (MQ=255)

                                                                     tGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAcg                                                                <  1:286352/140‑1 (MQ=255)

                                                                          cccGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                           <  2:847314/140‑1 (MQ=255)

                                                                          cccGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                           <  2:229603/140‑1 (MQ=255)

                                                                          cccGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                           <  1:379035/140‑1 (MQ=255)

                                                                           ccGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTc                                                          >  1:9280/1‑140 (MQ=255)

                                                                                  ttACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGcgcg                                                   <  1:362759/140‑1 (MQ=255)

                                                                                  ttACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGcgcg                                                   <  1:708629/140‑1 (MQ=255)

                                                                                        cGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAtgt                                             >  1:748481/1‑140 (MQ=255)

                                                                                          aCTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAtgtgt                                           >  2:347274/1‑139 (MQ=255)

                                                                                          aCTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATgtgg                                           >  2:682545/1‑140 (MQ=255)

                                                                                           cTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATgtggt                                          <  1:771434/140‑1 (MQ=255)

                                                                                                   tgtgGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATg                                   >  2:552631/1‑140 (MQ=255)

                                                                                                   tgtgGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATg                                   <  1:397960/140‑1 (MQ=255)

                                                                                                       gggTGTGTCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAAcc                               >  2:544131/1‑140 (MQ=255)

                                                                                                            tgtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                          >  1:825366/1‑140 (MQ=255)

                                                                                                            tgtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                          >  1:193971/1‑140 (MQ=255)

                                                                                                            tgtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                          >  2:435195/1‑140 (MQ=255)

                                                                                                            tgtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                          >  2:160652/1‑140 (MQ=255)

                                                                                                            tgtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                          >  1:786549/1‑140 (MQ=255)

                                                                                                             gtCATGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAc                         <  2:825366/140‑1 (MQ=255)

                                                                                                                 tGCGCCATTGAAACTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGt                     >  2:536374/1‑140 (MQ=255)

                                                                                                                           aaaCTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAAt           <  1:136363/140‑1 (MQ=255)

                                                                                                                             aCTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCt         <  2:357045/140‑1 (MQ=255)

                                                                                                                             aCTGCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCt         <  2:193971/140‑1 (MQ=255)

                                                                                                                                gCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCg      >  2:370666/1‑140 (MQ=255)

                                                                                                                                gCCGGAGACGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCg      >  2:310859/1‑140 (MQ=255)

                                                                                                                                    gagaCGAACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTg  <  2:748481/140‑1 (MQ=255)

CATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGC‑GGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTG  >  NC_000913/4146590‑4146860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A