Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,330,424	G→T	intergenic (+28/+158)	dacB → / ← obgE	peptidoglycan DD‑endopeptidase DacB/GTPase ObgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,424	0	G	T	100.0%	367.3 / NA	104	intergenic (+28/+158)	dacB/obgE	peptidoglycan DD‑endopeptidase DacB/GTPase ObgE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (57/47); total (57/47)

Only 100 of 105 total aligned reads displayed.

CAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCT  >  NC_000913/3330289‑3330558

cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCt                                                                                                                                    <  2:941955/140‑1 (MQ=255)

      cTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGat                                                                                                                              <  1:130147/140‑1 (MQ=255)

             gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGAc                                                                                                                       >  2:158677/1‑140 (MQ=255)

             gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGAc                                                                                                                       >  1:358514/1‑140 (MQ=255)

             gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGAc                                                                                                                       <  2:1466922/140‑1 (MQ=255)

               tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACaa                                                                                                                     <  1:679895/140‑1 (MQ=255)

                aTGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACaaa                                                                                                                    >  2:595060/1‑140 (MQ=255)

                aTGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACaaa                                                                                                                    >  1:1122471/1‑140 (MQ=255)

                 tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAg                                                                                                                   >  1:897343/1‑140 (MQ=255)

                 tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAg                                                                                                                   >  2:960321/1‑140 (MQ=255)

                  gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGt                                                                                                                  <  2:810199/140‑1 (MQ=255)

                   cAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTg                                                                                                                 >  2:1517561/1‑140 (MQ=255)

                      tAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTgcgc                                                                                                              <  2:1180851/140‑1 (MQ=255)

                           ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                         >  2:243179/1‑140 (MQ=255)

                           ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                         >  2:1335014/1‑140 (MQ=255)

                           ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                         >  1:108201/1‑140 (MQ=255)

                           ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                         >  1:736441/1‑140 (MQ=255)

                             tGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGttt                                                                                                       >  2:1083752/1‑140 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGTCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGTTTTGtttt                                                                                                      >  2:13735/1‑139 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCt                                                                                                             <  2:39677/133‑1 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCt                                                                                                             >  1:39677/1‑133 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTa                                                                                                      >  1:645609/1‑140 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTa                                                                                                      >  2:621232/1‑140 (MQ=255)

                              gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGCGCGCTTTGTTTa                                                                                                      >  2:1511907/1‑140 (MQ=255)

                                ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTAtt                                                                                                    >  2:715270/1‑139 (MQ=255)

                                  aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGcc                                                                                                  >  2:444053/1‑140 (MQ=255)

                                      gCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGAt                                                                                              <  2:628589/140‑1 (MQ=255)

                                       cGTAATCGCCGTAGTCCGTTAGAGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAATAATTAGTGAAAAAGAAACCCCGGCACATGGCCGTGGCTTCCGATTATTGACAAAGTACGCTTTGTTTATGCCGAATg                                                                                             >  2:350433/1‑140 (MQ=255)

                                         tAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTAGGATAACAATTAGTCAACATGAAACCCCTGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                           >  2:1053225/1‑140 (MQ=38)

                                            tCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggct                                                                                        >  2:41478/1‑139 (MQ=255)

                                            tCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                        >  1:736980/1‑140 (MQ=255)

                                             cGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt                                                                                       <  1:595060/140‑1 (MQ=255)

                                              gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                                                      <  2:1051173/140‑1 (MQ=255)

                                              gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                                                      <  2:258208/140‑1 (MQ=255)

                                                cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                                                                                    >  2:241678/1‑140 (MQ=255)

                                                  tATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACg                                                                                  <  1:1564281/140‑1 (MQ=255)

                                                         ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                           <  1:151009/140‑1 (MQ=255)

                                                         ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                           >  1:224968/1‑140 (MQ=255)

                                                         ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATAAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                           >  1:1580197/1‑140 (MQ=255)

                                                           aGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                         <  2:645609/140‑1 (MQ=255)

                                                           aGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                         <  2:606579/140‑1 (MQ=255)

                                                           aGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                         <  2:417576/140‑1 (MQ=255)

                                                            gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                        >  1:1303937/1‑140 (MQ=255)

                                                             tGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                       >  1:119071/1‑140 (MQ=255)

                                                                 ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  2:133705/1‑140 (MQ=255)

                                                                 ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  1:1539406/1‑140 (MQ=255)

                                                                 ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  2:1199851/1‑140 (MQ=255)

                                                                       aaGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc                                                             >  2:1448315/1‑140 (MQ=255)

                                                                           cGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                                                         <  1:1083752/140‑1 (MQ=255)

                                                                           cGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                                                         <  1:1328950/140‑1 (MQ=255)

                                                                           cGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                                                         <  2:224968/140‑1 (MQ=255)

                                                                           cGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                                                         <  1:1448315/140‑1 (MQ=255)

                                                                             tttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaa                                                       >  1:1493097/1‑140 (MQ=255)

                                                                               tGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                     <  1:1568571/140‑1 (MQ=255)

                                                                                     aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                               <  2:448507/140‑1 (MQ=255)

                                                                                     aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                               <  2:761231/140‑1 (MQ=255)

                                                                                       gATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatt                                             >  2:653366/1‑139 (MQ=255)

                                                                                        atatTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                               <  2:483004/137‑1 (MQ=255)

                                                                                        atatTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                               >  1:483004/1‑137 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  2:889125/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  2:885015/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  2:330529/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  2:253173/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  2:583935/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  1:1577242/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc                                         >  1:891202/1‑140 (MQ=255)

                                                                                           tttATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTACAAATTCAACATATTGc                                         >  2:1576777/1‑140 (MQ=255)

                                                                                              aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTATAAAATCGTGCAAATTCAACATATTGCAAt                                      <  1:1398871/140‑1 (MQ=255)

                                                                                              aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAt                                      <  2:358514/140‑1 (MQ=255)

                                                                                               tCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAtt                                     <  2:240966/140‑1 (MQ=255)

                                                                                               tCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAtt                                     <  1:1199851/140‑1 (MQ=255)

                                                                                                  gAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACTAAGTGAGCTTTGATTATGCCGGATGTGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtctc                                  >  2:358449/1‑140 (MQ=25)

                                                                                                  gAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtctc                                  >  1:1246371/1‑140 (MQ=255)

                                                                                                   aaCAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTctct                                 >  2:687387/1‑140 (MQ=255)

                                                                                                   aaCAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTctct                                 >  2:337963/1‑140 (MQ=255)

                                                                                                   aaCAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTctct                                 >  2:1420547/1‑140 (MQ=255)

                                                                                                    aCAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCtt                                >  2:239300/1‑140 (MQ=255)

                                                                                                     cAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  2:1122471/140‑1 (MQ=255)

                                                                                                     cAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  2:1580197/140‑1 (MQ=255)

                                                                                                     cAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  2:1539406/140‑1 (MQ=255)

                                                                                                     cAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               >  1:6082/1‑140 (MQ=255)

                                                                                                     cAATTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  1:133705/140‑1 (MQ=255)

                                                                                                     aaaTTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  1:19686/139‑1 (MQ=255)

                                                                                                     aaaTTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                               <  2:119071/139‑1 (MQ=255)

                                                                                                       aTTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa                             <  1:330529/140‑1 (MQ=255)

                                                                                                       aTTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa                             <  1:476438/140‑1 (MQ=255)

                                                                                                       aTTAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa                             <  2:787010/140‑1 (MQ=255)

                                                                                                        ttAGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAg                            >  1:463196/1‑140 (MQ=255)

                                                                                                          aGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                          <  2:108201/140‑1 (MQ=255)

                                                                                                          aGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                          <  1:444053/140‑1 (MQ=255)

                                                                                                          aGTCAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                          <  2:846212/140‑1 (MQ=255)

                                                                                                             cAAAAAGAAACCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTg                       >  1:529501/1‑140 (MQ=255)

                                                                                                                     aaCCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAGATATTGCAATTCTCTTGTAGGCCTGATAAGCGt               >  2:624806/1‑140 (MQ=255)

                                                                                                                      aCCCCGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTa              >  1:677345/1‑140 (MQ=255)

                                                                                                                       ccccGGCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAg             <  1:889125/140‑1 (MQ=255)

                                                                                                                           ggCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCa         <  2:897343/140‑1 (MQ=255)

                                                                                                                            gCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt        <  2:736441/140‑1 (MQ=255)

                                                                                                                            gCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt        >  2:102738/1‑140 (MQ=255)

                                                                                                                            gCACATGGCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt        <  1:1576777/140‑1 (MQ=255)

                                                                                                                                  ggCCGTGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt  >  2:1329422/1‑140 (MQ=255)

CAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCT  >  NC_000913/3330289‑3330558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A