Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F48 I1 R1 79 90.7 3162666 99.0% 3131039 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,952,677 C→A A65D (GCC→GAC)  ilvE → branched‑chain‑amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,6770CA100.0% 207.9 / NA 59A65D (GCC→GAC) ilvEbranched‑chain‑amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/35);  total (24/35)

AGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCATGGGA  >  NC_000913/3952540‑3952810
                                                                                                                                         |                                                                                                                                     
aGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACa                                                                                                                                     >  1:1459986/1‑140 (MQ=255)
       aaGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTa                                                                                                                              >  2:687209/1‑140 (MQ=255)
        aGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTAt                                                                                                                             <  1:1521022/140‑1 (MQ=255)
                  gATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCgg                                                                                                                   <  2:1115462/140‑1 (MQ=255)
                    tGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGtt                                                                                                                 <  1:694568/140‑1 (MQ=255)
                       cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCg                                                                                                              <  1:687209/140‑1 (MQ=255)
                         cACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCa                                                                                                            >  1:1270628/1‑140 (MQ=255)
                         cACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGGTTCTGCGACTCGCAAAAATGACCGTTTGTATTCCGCCATCGTGGGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCa                                                                                                            >  2:1054991/1‑140 (MQ=255)
                            gcgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCagag                                                                                                         <  2:1152890/140‑1 (MQ=255)
                            gcgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCagag                                                                                                         <  2:1284448/140‑1 (MQ=255)
                              gcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCa                                                                                                       <  2:1053771/140‑1 (MQ=255)
                                   aCTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATtgat                                                                                                  <  1:862229/140‑1 (MQ=255)
                                   aCTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATtgat                                                                                                  <  1:1277413/140‑1 (MQ=255)
                                   aCTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATtgat                                                                                                  <  2:766649/140‑1 (MQ=255)
                                   aCTATGGCACTTCGGTTTTTGAAGGCATACGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCACGGTTTCGCAGAGCATtgat                                                                                                  <  2:1486793/140‑1 (MQ=255)
                                      aTGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAg                                                                                               >  2:414303/1‑140 (MQ=255)
                                      aTGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAg                                                                                               <  2:458164/140‑1 (MQ=255)
                                      aTGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAg                                                                                               <  1:872926/140‑1 (MQ=255)
                                        ggCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCt                                                                                             >  2:1482138/1‑140 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGc                                                                                                        >  2:1192974/1‑125 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGc                                                                                                        <  1:1192974/125‑1 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATtg                                                                                                    >  2:1280726/1‑129 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATtg                                                                                                    <  1:1280726/129‑1 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGAtt                                                                                         >  2:1143/1‑139 (MQ=255)
                                            cTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATg                                                                                         <  2:614704/140‑1 (MQ=255)
                                              tCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGa                                                                                       <  2:301308/140‑1 (MQ=255)
                                                 gTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGc                                                                                    >  1:280124/1‑140 (MQ=255)
                                                  tttttGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCt                                                                                   >  1:1121191/1‑140 (MQ=255)
                                                           gCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTTATGGAAGCTTGTcgtgac                                                                          >  1:657547/1‑140 (MQ=255)
                                                               ccGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTcgtgacgtga                                                                      <  2:1301079/140‑1 (MQ=255)
                                                                   tGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCg                                                                  <  2:689007/140‑1 (MQ=255)
                                                                                  aaaGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCAc                                                   <  1:1527950/140‑1 (MQ=255)
                                                                                    aGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCa                                                 <  2:828241/140‑1 (MQ=255)
                                                                                    aGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCa                                                 <  1:414303/140‑1 (MQ=255)
                                                                                    aGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCa                                                 <  2:1118486/140‑1 (MQ=255)
                                                                                      gACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAgc                                               <  1:625064/140‑1 (MQ=255)
                                                                                        ccGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAgcgc                                             <  2:1121191/140‑1 (MQ=255)
                                                                                        ccGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAgcgc                                             <  1:632230/140‑1 (MQ=255)
                                                                                              gTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTatat                                       >  2:1423735/1‑140 (MQ=255)
                                                                                                 ttCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATAtcct                                    >  2:427240/1‑139 (MQ=255)
                                                                                                     gCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATAtccgtccg                                <  1:1247066/140‑1 (MQ=255)
                                                                                                             gAGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCAGTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCtt                        <  2:1112277/140‑1 (MQ=255)
                                                                                                              aGCATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTTCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTtc                       >  2:700611/1‑140 (MQ=255)
                                                                                                                cATATGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTtcgt                     >  1:1332403/1‑140 (MQ=255)
                                                                                                                   atGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCgg                  <  2:280124/140‑1 (MQ=255)
                                                                                                                   atGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCgg                  <  2:541701/140‑1 (MQ=255)
                                                                                                                   atGCAGCGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCgg                  <  1:1482138/140‑1 (MQ=255)
                                                                                                                   atGCAGCGTCTGCATGAATCCGAAAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCgg                  <  1:612099/140‑1 (MQ=255)
                                                                                                                         cGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGt            >  1:1434900/1‑140 (MQ=255)
                                                                                                                         cGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGt            >  2:495121/1‑140 (MQ=255)
                                                                                                                         cGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGt            >  1:1560529/1‑140 (MQ=255)
                                                                                                                         cGTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGt            <  1:5912/140‑1 (MQ=255)
                                                                                                                          gTCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGtt           >  2:14355/1‑140 (MQ=255)
                                                                                                                           tCTGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACATGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTTGTCGGTGATGTTg          >  1:740759/1‑140 (MQ=255)
                                                                                                                             tGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGc        >  1:1437760/1‑140 (MQ=255)
                                                                                                                             tGCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGc        >  1:1018558/1‑140 (MQ=255)
                                                                                                                              gCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCa       >  1:1469725/1‑140 (MQ=255)
                                                                                                                              gCATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCa       <  1:334201/140‑1 (MQ=255)
                                                                                                                               cATGACTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCAt      <  2:1560529/140‑1 (MQ=255)
                                                                                                                                   aCTCCGACAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCATGGGa  <  1:1394694/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
AGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTCCCGGTTTCGCAGAGCATTGATGAGCTGATGGAAGCTTGTCGTGACGTGATCCGCAAAAACAATCTCACCAGCGCCTATATCCGTCCGCTGATCTTCGTCGGTGATGTTGGCATGGGA  >  NC_000913/3952540‑3952810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A