Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,046	C→T	intergenic (+77/+253)	yiiF → / ← fdhE	protein YiiF/formate dehydrogenase formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,046	0	C	T	99.1%	399.3 / ‑4.2	115	intergenic (+77/+253)	yiiF/fdhE	protein YiiF/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (43/71); total (43/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 122 total aligned reads displayed.

CTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGA  >  NC_000913/4079909‑4080180

cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTgg                                                                                                                                      >  2:453556/1‑140 (MQ=255)

cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTgg                                                                                                                                      >  2:547491/1‑140 (MQ=255)

cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTgg                                                                                                                                      >  2:1335653/1‑140 (MQ=255)

  ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGcc                                                                                                                                    >  1:1204599/1‑140 (MQ=255)

    cAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTa                                                                                                                                  <  1:422426/140‑1 (MQ=255)

     aaGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTAc                                                                                                                                 <  1:1395723/140‑1 (MQ=255)

      aGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTTACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACa                                                                                                                                <  1:1334898/140‑1 (MQ=255)

      aGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACa                                                                                                                                <  1:453556/140‑1 (MQ=255)

      aGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACa                                                                                                                                <  1:460090/140‑1 (MQ=255)

      aGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                                                                                                <  2:1133098/140‑1 (MQ=255)

       gggTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACaa                                                                                                                               <  1:1350757/140‑1 (MQ=255)

           gtgAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGATAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACCCCTTATCTGGCCTAAAAAGtt                                                                                                                           >  2:793547/1‑139 (MQ=255)

            tgAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCg                                                                                                                          >  2:805012/1‑140 (MQ=255)

             gAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGt                                                                                                                         >  1:151237/1‑140 (MQ=255)

               ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGtat                                                                                                                       <  1:1122196/140‑1 (MQ=255)

               ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGtat                                                                                                                       <  1:344077/140‑1 (MQ=255)

               ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGtat                                                                                                                       <  1:1268192/140‑1 (MQ=255)

                  aGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATaaa                                                                                                                    >  1:1175465/1‑140 (MQ=255)

                   gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAAt                                                                                                                   >  2:207653/1‑140 (MQ=255)

                   gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAAt                                                                                                                   >  1:1455155/1‑140 (MQ=255)

                   gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAAt                                                                                                                   >  1:1189071/1‑140 (MQ=255)

                    aTGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAAtt                                                                                                                  <  1:1237466/140‑1 (MQ=255)

                    aTGGTGTCGAATATCAGCATAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAAACAGATTGCTGACATCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGGCTGATCAGGCCTAGAAACTCGTATAAAtt                                                                                                                  <  2:1107994/140‑1 (MQ=37)

                        tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAAt                                                                                                              >  1:1479100/1‑140 (MQ=255)

                        tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAAt                                                                                                              >  2:1036746/1‑140 (MQ=255)

                              atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGGCTACAAAGTCGTATAAATTCAATGTATTg                                                                                                        <  2:1284036/140‑1 (MQ=255)

                              atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTg                                                                                                        <  1:1473343/140‑1 (MQ=255)

                              atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTg                                                                                                        <  1:1335653/140‑1 (MQ=255)

                               tatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                       <  1:1097611/140‑1 (MQ=255)

                               tatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                       <  1:569826/140‑1 (MQ=255)

                                  cAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTg                                                                                                        >  2:387239/1‑136 (MQ=255)

                                  cAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTg                                                                                                        <  1:387239/136‑1 (MQ=255)

                                    gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGttt                                                                                                  <  1:358986/140‑1 (MQ=255)

                                    gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGttt                                                                                                  <  2:1147662/140‑1 (MQ=255)

                                       tAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAtt                                                                                               >  2:628406/1‑140 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgt                                                                                             >  2:514666/1‑139 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  2:1189071/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  1:674904/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  2:1101911/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  2:121761/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  2:1204599/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  1:1011331/140‑1 (MQ=255)

                                         aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                             <  1:1389600/140‑1 (MQ=255)

                                          gCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGt                                                                                            <  1:805012/140‑1 (MQ=255)

                                          gCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGt                                                                                            >  2:1314452/1‑140 (MQ=255)

                                           tttctctaGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGAGCTCGTTGTTCAAGCTGGATGCAACGTGAACGCCTTATGTGGCCTACAAAATCGTATAAAAGCAAAGTATTGCAGTTAATTGGTa                                                                                           <  2:889922/133‑1 (MQ=255)

                                           cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTa                                                                                           <  1:1001722/140‑1 (MQ=255)

                                             gcgcGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAgg                                                                                         <  2:796468/140‑1 (MQ=255)

                                              cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGc                                                                                        >  2:675124/1‑140 (MQ=255)

                                              cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGc                                                                                        <  1:157514/140‑1 (MQ=255)

                                               gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                       >  1:59366/1‑140 (MQ=255)

                                               gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                       >  1:1051142/1‑140 (MQ=255)

                                               gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                       >  2:1032154/1‑140 (MQ=255)

                                                   ggAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGAt                                                                                   <  1:881959/140‑1 (MQ=255)

                                                             tGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagcg                                                                         >  2:521165/1‑140 (MQ=255)

                                                              gggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagcgc                                                                        <  1:275513/140‑1 (MQ=255)

                                                               ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                       <  1:1525787/140‑1 (MQ=255)

                                                               ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                       >  1:1574394/1‑140 (MQ=255)

                                                               ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                       >  1:1158507/1‑140 (MQ=255)

                                                               ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCAGGATGCGGCGTGAACTCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                       >  2:1574411/1‑140 (MQ=255)

                                                                   aaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGG‑AGGCCTGATAAGCGCAGCGCATCAgg                                                                  <  2:1455155/140‑1 (MQ=255)

                                                                    aaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                  <  2:885919/140‑1 (MQ=255)

                                                                    aaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                  <  1:343247/140‑1 (MQ=255)

                                                                         atgTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAAtt                                                             >  2:928712/1‑139 (MQ=255)

                                                                           gTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCtt                                                           >  2:184742/1‑140 (MQ=255)

                                                                            tCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTa                                                          <  1:252112/140‑1 (MQ=255)

                                                                              aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAA‑GTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGc                                                       <  1:1482954/140‑1 (MQ=255)

                                                                                tCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCt                                                      <  2:1479100/140‑1 (MQ=255)

                                                                                tCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCt                                                      <  2:1051142/140‑1 (MQ=255)

                                                                                             aaCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtctc                                         >  2:903404/1‑140 (MQ=255)

                                                                                             aaCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtctc                                         <  2:1298915/140‑1 (MQ=255)

                                                                                              aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                        <  1:921640/140‑1 (MQ=255)

                                                                                              aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                        <  1:207653/140‑1 (MQ=255)

                                                                                              aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                        <  1:903404/140‑1 (MQ=255)

                                                                                              aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                        <  1:70162/140‑1 (MQ=255)

                                                                                              aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                        >  1:644480/1‑140 (MQ=255)

                                                                                                  gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                    >  2:832459/1‑140 (MQ=255)

                                                                                                  gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                    >  2:869425/1‑140 (MQ=255)

                                                                                                   cgcgTTGTTCATGCCGTATGCGGCGTGAACGCCTTATCTGGCCTACAAAGGCGTATAAATTCAATGTATTGCAGTTTATTGGTATGCCTGATAAGCGCAGCGCATCAGGCAATCTTACTCTTGTCATCAGTCTCATGccc                                   >  2:205441/1‑140 (MQ=25)

                                                                                                   cgcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCACCAGTCTCATGccc                                   <  1:882345/140‑1 (MQ=255)

                                                                                                    gcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCa                                  <  2:1574394/140‑1 (MQ=255)

                                                                                                    gcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCa                                  <  2:1158507/140‑1 (MQ=255)

                                                                                                     cgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                                 <  1:1036746/140‑1 (MQ=255)

                                                                                                     cgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                                 <  1:628406/140‑1 (MQ=255)

                                                                                                     cgTTGATCATGCCGGATGCGGCGTGAACGCCTTATCTGGTCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCAACAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                                 <  2:152072/140‑1 (MQ=255)

                                                                                                       ttgttCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                               <  1:1222131/140‑1 (MQ=255)

                                                                                                        tgttCATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAc                              <  1:1303568/140‑1 (MQ=255)

                                                                                                            cATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCtt                           >  2:1538211/1‑139 (MQ=255)

                                                                                                            cATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCtt                           <  1:1538211/139‑1 (MQ=255)

                                                                                                            cATGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTa                          >  2:6314/1‑140 (MQ=255)

                                                                                                             aTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                         <  1:413560/140‑1 (MQ=255)

                                                                                                             aTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                         <  2:535409/140‑1 (MQ=255)

                                                                                                             aTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                         <  2:593236/140‑1 (MQ=255)

                                                                                                             aTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                         >  2:1112442/1‑140 (MQ=255)

                                                                                                             aTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                         >  2:1170196/1‑140 (MQ=255)

                                                                                                              tGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAtt                        >  2:1041411/1‑140 (MQ=255)

                                                                                                                  agATGCGGCGTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                    <  2:24227/139‑1 (MQ=255)

                                                                                                                      gcggcgTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggt                <  1:293927/140‑1 (MQ=255)

                                                                                                                        ggcgTGAACGCCTTATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtgg              <  1:198/140‑1 (MQ=255)

                                                                                                                                    ttATCTGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGa  >  2:1172240/1‑140 (MQ=255)

CTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGA  >  NC_000913/4079909‑4080180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A