Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F48 I1 R1	62	93.4	3176452	99.2%	3151040	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,942	C→A	G279V (GGA→GTA)	truB ←	tRNA pseudouridine(55) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,942	0	C	A	99.2%	444.2 / NA	126	G279V (GGA→GTA)	truB	tRNA pseudouridine(55) synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (49/76); minor base G (0/1); total (49/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTCCTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTC  >  NC_000913/3311805‑3312075

gCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACt                                                                                                                                     >  1:34367/1‑140 (MQ=255)

 cGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACtt                                                                                                                                    >  2:1039129/1‑140 (MQ=255)

  gACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTc                                                                                                                                   <  1:70807/140‑1 (MQ=255)

  gACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTc                                                                                                                                   >  1:338696/1‑140 (MQ=255)

        ttATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGTCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTgg                                                                                                                             <  1:531850/140‑1 (MQ=255)

        ttATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTgg                                                                                                                             <  2:1085603/140‑1 (MQ=255)

        ttATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTgg                                                                                                                             <  1:135614/140‑1 (MQ=255)

          aTCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGgcg                                                                                                                           <  2:306687/140‑1 (MQ=255)

             gCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGTCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCAc                                                                                                                        <  2:1335351/140‑1 (MQ=255)

             gCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCAc                                                                                                                        <  1:335563/140‑1 (MQ=255)

               aaGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCa                                                                                                                      <  1:275124/140‑1 (MQ=255)

                aGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAg                                                                                                                     <  1:630688/140‑1 (MQ=255)

                aGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAg                                                                                                                     <  2:584833/140‑1 (MQ=255)

                  aCGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGAt                                                                                                                   >  2:1364059/1‑140 (MQ=255)

                     gTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTa                                                                                                                >  1:1282325/1‑140 (MQ=255)

                         aCATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGaa                                                                                                            <  2:935183/140‑1 (MQ=255)

                         aCATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGaa                                                                                                            <  2:857829/140‑1 (MQ=255)

                         aCATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGaa                                                                                                            <  1:1364059/140‑1 (MQ=255)

                          cATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAAc                                                                                                           <  1:1189038/140‑1 (MQ=255)

                           aTTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAAcc                                                                                                          <  2:333510/140‑1 (MQ=255)

                               cGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGt                                                                                                      >  2:740912/1‑140 (MQ=255)

                               cGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGt                                                                                                      >  1:131698/1‑140 (MQ=255)

                                gCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGtt                                                                                                     >  2:1051093/1‑140 (MQ=255)

                                gCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGtt                                                                                                     >  2:540818/1‑140 (MQ=255)

                                  cGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTGCTTCCAGTGGCGCACCAGATGTACGAACCGGGTTAc                                                                                                   <  1:446125/140‑1 (MQ=255)

                                  cGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTAc                                                                                                   <  2:1028167/140‑1 (MQ=255)

                                  cGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTAc                                                                                                   <  2:433565/140‑1 (MQ=255)

                                    ggTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCa                                                                                                 >  2:166807/1‑140 (MQ=255)

                                    ggTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCa                                                                                                 >  1:917852/1‑140 (MQ=255)

                                    ggTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCa                                                                                                 >  1:109177/1‑140 (MQ=255)

                                     gTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCAt                                                                                                >  2:1464922/1‑140 (MQ=255)

                                         tCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCAttttt                                                                                            <  2:29126/140‑1 (MQ=255)

                                            accaccAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGaa                                                                                         >  2:216488/1‑140 (MQ=255)

                                              caccaGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCAttttt                                                                                            <  2:642673/135‑1 (MQ=255)

                                              caccaGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCAttttt                                                                                            >  1:642673/1‑135 (MQ=255)

                                               accaGGCGACTAGGCTCACCGCGGCCTTCATCGGCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGACCCAGTACGTCCAATGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTa                                                                                      <  2:131698/140‑1 (MQ=255)

                                                ccaGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTaa                                                                                     >  1:738974/1‑140 (MQ=255)

                                                ccaGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTaa                                                                                     >  2:751824/1‑140 (MQ=255)

                                                ccaGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTaa                                                                                     >  2:170475/1‑140 (MQ=255)

                                                 caGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTaaa                                                                                    <  1:221435/140‑1 (MQ=255)

                                                 caGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTaaa                                                                                    <  2:1266957/140‑1 (MQ=255)

                                                  aGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCAttttt                                                                                            <  2:430636/131‑1 (MQ=255)

                                                   ggCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACa                                                                                  >  2:793610/1‑140 (MQ=255)

                                                   ggCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACa                                                                                  >  1:960212/1‑140 (MQ=255)

                                                   ggCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACa                                                                                  >  1:88673/1‑140 (MQ=255)

                                                   ggCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACa                                                                                  >  2:135699/1‑140 (MQ=255)

                                                     cgacgaGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACaga                                                                                <  1:166807/140‑1 (MQ=255)

                                                      gacgaGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACagaa                                                                               <  2:1239420/140‑1 (MQ=255)

                                                        cgaGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACagaaga                                                                             >  1:67519/1‑140 (MQ=255)

                                                                aaCGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACg                                                                     <  2:288341/140‑1 (MQ=255)

                                                                aaCGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACg                                                                     <  2:379302/140‑1 (MQ=255)

                                                                     ggCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGa                                                                <  2:843337/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  2:642686/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  1:1039129/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  1:1411553/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  2:713758/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  1:1464922/140‑1 (MQ=255)

                                                                       ccTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGAtt                                                              <  2:872208/140‑1 (MQ=255)

                                                                                 cAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGa                                                    >  1:315966/1‑140 (MQ=255)

                                                                                 cAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGa                                                    <  2:917852/140‑1 (MQ=255)

                                                                                 cAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGa                                                    >  1:448880/1‑140 (MQ=255)

                                                                                      tCGCTCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  1:48479/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  2:109177/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  2:27891/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  1:608925/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  1:877878/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  1:997150/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTc                                               <  2:315966/140‑1 (MQ=255)

                                                                                      tCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGAAGTTAACGGAAGATTCACCACCGGATAGTc                                               <  1:1405002/140‑1 (MQ=255)

                                                                                           cATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAg                                          <  2:1271165/140‑1 (MQ=255)

                                                                                           cATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAg                                          <  1:1051093/140‑1 (MQ=255)

                                                                                           aaTACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAg                                          <  2:238507/139‑1 (MQ=255)

                                                                                            aTACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc                                         >  1:515430/1‑140 (MQ=255)

                                                                                                cGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGa                                     <  2:960212/140‑1 (MQ=255)

                                                                                                cGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGa                                     <  2:34367/140‑1 (MQ=255)

                                                                                                cGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGa                                     <  2:338403/140‑1 (MQ=255)

                                                                                                 gATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGAc                                    <  1:814863/140‑1 (MQ=255)

                                                                                                       tttGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTGCGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCa                              <  1:913624/140‑1 (MQ=255)

                                                                                                       tttGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCa                              >  2:639673/1‑140 (MQ=255)

                                                                                                         tGCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCAtt                            <  2:1229493/140‑1 (MQ=255)

                                                                                                          gCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTg                           >  1:286124/1‑140 (MQ=255)

                                                                                                          gCCGTTCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTg                           >  2:1016212/1‑140 (MQ=255)

                                                                                                              ttatcACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  2:473898/137‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  1:540818/140‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  2:497782/140‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  2:513296/140‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  2:515430/140‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  1:780525/140‑1 (MQ=255)

                                                                                                              ttCTCACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCAt                       <  2:1433055/140‑1 (MQ=255)

                                                                                                              ccctctCCTTCCGTGACGCGAACTAGTTCTTCCAGTGGCGCACCAGATGTACGAAGCGGGTTACCATTTTTGAAGTAAACAGTAGACGTTAACGGAAGACTTACCAGCGGATGGTCTGAAGCTGGACTGTCCGTTGGCAt                       <  2:347507/138‑1 (MQ=255)

                                                                                                                ctcACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCa                     >  1:859406/1‑140 (MQ=255)

                                                                                                                ctcACCTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCa                     >  2:358310/1‑140 (MQ=255)

                                                                                                                    ccTTCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTaa                 <  1:1088067/140‑1 (MQ=255)

                                                                                                                      ttCCGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg               <  1:216488/140‑1 (MQ=255)

                                                                                                                        ccGTGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTGCCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGa             >  1:290459/1‑140 (MQ=255)

                                                                                                                           tGACGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCa          >  2:535213/1‑140 (MQ=255)

                                                                                                                             aCGCGAACCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGt                   <  2:492541/129‑1 (MQ=255)

                                                                                                                                  aaCCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACt   >  2:63270/1‑140 (MQ=255)

                                                                                                                                   aCCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTc  >  1:781729/1‑140 (MQ=255)

                                                                                                                                   aCCAGTACTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTAGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTc  >  2:1532167/1‑140 (MQ=255)

GCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTCCTTCCAGTGGCGCACCAGATGTACGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTC  >  NC_000913/3311805‑3312075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A