Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F48 I1 R1	62	93.4	3176452	99.2%	3151040	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,994,856	G→T	R32L (CGC→CTC)	dapF →	diaminopimelate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,994,856	0	G	T	98.5%	231.2 / NA	66	R32L (CGC→CTC)	dapF	diaminopimelate epimerase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (31/34); minor base A (0/1); total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGTGCGGCAACGGTG  >  NC_000913/3994719‑3994990

ttACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTcc                                                                                                                                      >  1:901242/1‑140 (MQ=255)

   cTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTgg                                                                                                                                   <  1:106727/140‑1 (MQ=255)

    tAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGc                                                                                                                                  >  1:563912/1‑140 (MQ=255)

     aaaaGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCt                                                                                                                                 >  2:1093286/1‑140 (MQ=255)

        aGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGAt                                                                                                                              >  1:99843/1‑140 (MQ=255)

         gTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATc                                                                                                                             <  2:980392/140‑1 (MQ=255)

         gTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATc                                                                                                                             <  2:1571054/140‑1 (MQ=255)

         gTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATc                                                                                                                             <  2:467796/140‑1 (MQ=255)

          tCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCg                                                                                                                            <  1:639286/140‑1 (MQ=255)

          tCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCg                                                                                                                            <  1:1474931/140‑1 (MQ=255)

           cAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCgg                                                                                                                           <  2:1443813/140‑1 (MQ=255)

               ttCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCAcc                                                                                                                       <  1:470354/140‑1 (MQ=255)

               ttCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCAcc                                                                                                                       <  2:188560/140‑1 (MQ=255)

               ttCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCAcc                                                                                                                       <  2:788270/140‑1 (MQ=255)

                    tACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGTTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTgggg                                                                                                                  >  1:969197/1‑140 (MQ=255)

                    tACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTgggg                                                                                                                  >  2:1273325/1‑140 (MQ=255)

                      cccGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCACCTGGCTGATCGGCACCTGGGGGt                                                                                                                <  1:369655/140‑1 (MQ=255)

                      cccGAGTGATAGGACTAAATGAAGAAGTTCTGCAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGACTCCTGGCTGATCGGCACCTGGGGGt                                                                                                                <  1:1181254/140‑1 (MQ=255)

                        cgcgTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAg                                                                                                              >  1:1458971/1‑140 (MQ=255)

                          cgTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAggg                                                                                                            >  1:1452937/1‑140 (MQ=255)

                          cgTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAggg                                                                                                            >  2:1552851/1‑140 (MQ=255)

                           gTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGt                                                                                                           <  1:468256/140‑1 (MQ=255)

                            tGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGtt                                                                                                          <  2:696282/140‑1 (MQ=255)

                            tGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGtt                                                                                                          <  2:1246014/140‑1 (MQ=255)

                                  gAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCa                                                                                                    <  1:1159564/140‑1 (MQ=255)

                                  gAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCa                                                                                                    <  1:1093286/140‑1 (MQ=255)

                                    gTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAAc                                                                                                  >  1:791034/1‑140 (MQ=255)

                                         tgatgCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAActgctg                                                                                             >  2:498199/1‑140 (MQ=255)

                                            tgCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCtggtg                                                                                          >  1:639273/1‑140 (MQ=255)

                                              cAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTggtggt                                                                                        >  2:1352211/1‑140 (MQ=255)

                                                  tctcGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAg                                                                                    <  2:1452937/140‑1 (MQ=255)

                                                  tctcGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAg                                                                                    <  1:1008614/140‑1 (MQ=255)

                                                   ctcGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgc                                                                                   <  2:287911/140‑1 (MQ=255)

                                                   ctcGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgc                                                                                   <  2:803822/140‑1 (MQ=255)

                                                   ctcGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgc                                                                                   >  1:995770/1‑140 (MQ=255)

                                                     cGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgccg                                                                                 <  1:1552851/140‑1 (MQ=255)

                                                     cGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgccg                                                                                 <  2:901242/140‑1 (MQ=255)

                                                     cGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACCCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgccg                                                                                 <  2:152983/140‑1 (MQ=255)

                                                      gAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAgccgc                                                                                >  1:1270057/1‑140 (MQ=255)

                                                         aaTGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGt                                                                             >  1:1440251/1‑140 (MQ=255)

                                                             cATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGa                                                                         >  1:120175/1‑140 (MQ=255)

                                                              aTGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGAt                                                                        >  2:899262/1‑140 (MQ=255)

                                                                     tGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAAc                                                                 <  2:99843/140‑1 (MQ=255)

                                                                     tGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAAc                                                                 >  1:204554/1‑140 (MQ=255)

                                                                            gATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGAttt                                                          >  2:217525/1‑140 (MQ=255)

                                                                            gATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGAttt                                                          >  1:446255/1‑140 (MQ=255)

                                                                                 tATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACt                                                     <  1:1137093/140‑1 (MQ=255)

                                                                                 tATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACt                                                     >  2:619627/1‑140 (MQ=255)

                                                                                      tcgtcgACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGc                                                >  2:1390224/1‑140 (MQ=255)

                                                                                      tcgtcgACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGc                                                <  2:537828/140‑1 (MQ=255)

                                                                                        gtcgACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCAt                                              >  1:964458/1‑140 (MQ=255)

                                                                                           gACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCAtttt                                           <  1:1273325/140‑1 (MQ=255)

                                                                                             cgcgGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCa                                         <  1:71950/140‑1 (MQ=255)

                                                                                              gcgGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCaa                                        >  1:1010389/1‑140 (MQ=255)

                                                                                                  tgaCGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAAATGCTGGTGGTTGAGCCGCCGTATGAACCTGAACTGGATTTTCACTATCGCATTTTCAATGCt                                    <  2:563912/138‑1 (MQ=255)

                                                                                                  tAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCt                                    <  1:899262/140‑1 (MQ=255)

                                                                                                  aaaCGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCt                                    <  2:237404/139‑1 (MQ=255)

                                                                                                     cGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGAt                                 <  1:619627/140‑1 (MQ=255)

                                                                                                     agCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGAt                                 <  2:446255/139‑1 (MQ=255)

                                                                                                            tGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTg                          >  2:271141/1‑140 (MQ=255)

                                                                                                            tGTCTTTTTTTCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTg                          >  2:1378951/1‑140 (MQ=255)

                                                                                                                tttttttCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGt                      >  1:519871/1‑140 (MQ=255)

                                                                                                                    tttCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGgcg                  <  1:498199/140‑1 (MQ=255)

                                                                                                                    tttCACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGgcg                  <  2:964458/140‑1 (MQ=255)

                                                                                                                       cACCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAg               <  1:217525/140‑1 (MQ=255)

                                                                                                                        aCCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGt              >  1:1444895/1‑140 (MQ=255)

                                                                                                                        aCCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGt              >  2:936190/1‑140 (MQ=255)

                                                                                                                        aCCGGAGCTGATTCGTCTCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGt              >  2:1141714/1‑140 (MQ=255)

                                                                                                                         attgAGCTGATTCGTCTCCTGGCTGAGCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTTGTTTAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGtt             <  2:984568/137‑2 (MQ=255)

                                                                                                                                    tcgtctCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGTGCGGCAACGGTg  >  2:780199/1‑140 (MQ=255)

TTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATCCTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGTGCGGCAACGGTG  >  NC_000913/3994719‑3994990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A