Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F48 I1 R1	70	151.6	5072932	98.7%	5006983	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,498,512	G→T	G229W (GGG→TGG)	cysG →	siroheme synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,498,512	0	G	T	99.4%	549.2 / ‑6.7	154	G229W (GGG→TGG)	cysG	siroheme synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (67/86); minor base A (0/1); total (67/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGAT  >  NC_000913/3498376‑3498647

   tAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGgctgc                                                                                                                                   >  2:2164028/1‑140 (MQ=255)

    aaCGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACCGAAACGACCGAAGAGTTAATCCACGAACCGCGGGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGgctgct                                                                                                                                  <  1:1676296/140‑1 (MQ=255)

     aCGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGctgctg                                                                                                                                 <  1:1921978/140‑1 (MQ=255)

     aCGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGctgctg                                                                                                                                 <  1:1578804/140‑1 (MQ=255)

      cGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGTACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGa                                                                                                                                <  1:2071729/140‑1 (MQ=255)

      cGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGa                                                                                                                                <  1:1091727/140‑1 (MQ=255)

       gACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGac                                                                                                                               <  2:713209/140‑1 (MQ=255)

         ccGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGacac                                                                                                                             <  2:1067286/140‑1 (MQ=255)

          cGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACt                                                                                                                            <  2:1820872/140‑1 (MQ=255)

            ccTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGa                                                                                                                          >  1:857785/1‑140 (MQ=255)

            ccTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGa                                                                                                                          >  1:2441379/1‑140 (MQ=255)

            ccTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGa                                                                                                                          <  1:569374/140‑1 (MQ=255)

             cTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGaa                                                                                                                         <  2:541156/140‑1 (MQ=255)

                 ggcAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGa                                                                                                                     <  2:638408/139‑1 (MQ=255)

                 cgcAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGa                                                                                                                     <  2:2184380/140‑1 (MQ=255)

                  gcAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGAc                                                                                                                    <  1:1106485/140‑1 (MQ=255)

                     gTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGc                                                                                                                 >  1:2493109/1‑140 (MQ=255)

                      tCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGca                                                                                                                >  2:1991615/1‑140 (MQ=255)

                      tCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGca                                                                                                                <  2:1719631/140‑1 (MQ=255)

                      tCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCAGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGca                                                                                                                >  1:464725/1‑140 (MQ=255)

                           ggCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACaaa                                                                                                           >  1:1490350/1‑140 (MQ=255)

                                acaacGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTcag                                                                                                      <  2:2441379/140‑1 (MQ=255)

                                acaacGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTcag                                                                                                      >  2:1394035/1‑140 (MQ=255)

                                  aacGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTcagca                                                                                                    >  1:205708/1‑140 (MQ=255)

                                  aacGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTcagca                                                                                                    <  2:1834750/140‑1 (MQ=255)

                                    cGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAgcagg                                                                                                  <  1:1122807/140‑1 (MQ=255)

                                      aTCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAgcaggca                                                                                                <  1:241727/140‑1 (MQ=255)

                                       tCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAgcaggcag                                                                                               <  2:1773931/140‑1 (MQ=255)

                                            aaGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGAtgtg                                                                                          <  1:477678/140‑1 (MQ=255)

                                            aaGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGAtgtg                                                                                          >  2:899626/1‑140 (MQ=255)

                                             aGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATgtgg                                                                                         <  1:2342434/140‑1 (MQ=255)

                                             aGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATgtgg                                                                                         <  1:1526040/140‑1 (MQ=255)

                                             aGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATgtgg                                                                                         <  2:857785/140‑1 (MQ=255)

                                             aGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATgtgg                                                                                         <  2:1466995/140‑1 (MQ=255)

                                             aGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATgtgg                                                                                         <  1:2526210/140‑1 (MQ=255)

                                                 aTTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTggtggt                                                                                     >  2:433983/1‑140 (MQ=255)

                                                 aTTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTggtggt                                                                                     <  2:1167327/140‑1 (MQ=255)

                                                  ttACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGtggtggcc                                                                                    >  1:2226985/1‑138 (MQ=255)

                                                  ttACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTc                                                                                    >  1:514849/1‑140 (MQ=255)

                                                   tACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCt                                                                                   >  1:1858073/1‑140 (MQ=255)

                                                        aaaCGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGAc                                                                              <  2:440815/140‑1 (MQ=255)

                                                         aaCGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGAcc                                                                             >  1:499999/1‑140 (MQ=255)

                                                         aaCGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGAcc                                                                             >  1:722419/1‑140 (MQ=255)

                                                         aaCGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGAcc                                                                             >  1:2209808/1‑140 (MQ=255)

                                                            gACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTc                                                                          <  2:1581845/140‑1 (MQ=255)

                                                             aCCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGCCt                                                                         >  1:51228/1‑140 (MQ=255)

                                                                     gTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTg                                                                 <  1:130281/140‑1 (MQ=255)

                                                                     gTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTg                                                                 <  1:1991615/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                >  1:1180862/1‑140 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  1:748451/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  1:382245/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  1:2154792/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCAGGCTGCTGACCCTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  2:2004079/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGATCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  1:899626/140‑1 (MQ=255)

                                                                      ttAATCAACGAACCGCTCGACCATCGCAGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  1:807807/140‑1 (MQ=255)

                                                                      atAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTCGTGCAGGTCCGGGCGATGCCTGGCTGCTGACTCTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGa                                                                <  2:2084581/139‑1 (MQ=255)

                                                                       tAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTTAc                                                               <  1:36997/140‑1 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              <  1:1404254/140‑1 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              <  2:51228/140‑1 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              <  2:2274160/140‑1 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              >  1:2096536/1‑140 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              <  2:205708/140‑1 (MQ=255)

                                                                        aaTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACg                                                              >  2:1282614/1‑140 (MQ=255)

                                                                         aTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTTAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGa                                                             <  1:1594394/140‑1 (MQ=255)

                                                                         aTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGa                                                             <  2:669347/140‑1 (MQ=255)

                                                                         aTCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGa                                                             <  1:2008708/140‑1 (MQ=255)

                                                                          acAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGat                                                            <  2:879573/139‑1 (MQ=255)

                                                                            aaCGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGatat                                                          <  1:901362/140‑1 (MQ=255)

                                                                             aCGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtatt                                                         >  1:520168/1‑140 (MQ=255)

                                                                             aCGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtatt                                                         <  1:2500405/140‑1 (MQ=255)

                                                                              cGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtatta                                                        >  1:230485/1‑140 (MQ=255)

                                                                              cGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtatta                                                        >  2:2453881/1‑140 (MQ=255)

                                                                               gAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtattat                                                       <  1:2300579/140‑1 (MQ=255)

                                                                               gAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtattat                                                       <  2:72206/140‑1 (MQ=255)

                                                                               gAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtattat                                                       <  2:499999/140‑1 (MQ=255)

                                                                               gAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGAtattat                                                       <  2:722419/140‑1 (MQ=255)

                                                                               gAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGGCTGGTTTCTGACGAtattat                                                       >  1:2100106/1‑140 (MQ=255)

                                                                                aaCCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATg                                                      >  1:1152875/1‑140 (MQ=255)

                                                                                aaCCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATg                                                      >  1:964176/1‑140 (MQ=255)

                                                                                    gCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATc                                                  >  2:2480340/1‑140 (MQ=255)

                                                                                        gACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGt                                              <  1:586572/140‑1 (MQ=255)

                                                                                             tCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTAcgcc                                         >  2:398231/1‑140 (MQ=255)

                                                                                             tCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTAcgcc                                         <  1:2389145/140‑1 (MQ=255)

                                                                                              cgcgGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTAcgccg                                        <  2:1107167/140‑1 (MQ=255)

                                                                                                cgGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCcgcg                                      >  2:2417130/1‑140 (MQ=255)

                                                                                                        tggtgCTGGTTGGTGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGAc                              >  1:1570557/1‑140 (MQ=255)

                                                                                                               ggttggtGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGttt                       >  1:1464152/1‑140 (MQ=255)

                                                                                                                     tGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTtcgtcg                 >  2:2527190/1‑140 (MQ=255)

                                                                                                                     tGCAGGTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTtcgtcg                 >  1:721230/1‑140 (MQ=255)

                                                                                                                          gTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCaaa            >  1:1735336/1‑140 (MQ=255)

                                                                                                                          gTCCGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCaaa            >  1:2375288/1‑140 (MQ=255)

                                                                                                                            ccGGGCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcg          <  2:925157/140‑1 (MQ=255)

                                                                                                                              gggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcg        <  2:1570557/140‑1 (MQ=255)

                                                                                                                              gggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcg        <  2:709676/140‑1 (MQ=255)

                                                                                                                              gggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcg        <  2:2339904/140‑1 (MQ=255)

                                                                                                                               ggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcgc       >  1:1688429/1‑140 (MQ=255)

                                                                                                                               ggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcgc       >  2:2164096/1‑140 (MQ=255)

                                                                                                                               ggCGATGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAAcgcg        >  1:2344002/1‑139 (MQ=255)

                                                                                                                                    tGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGAt  >  1:1864323/1‑140 (MQ=255)

                                                                                                                                    tGCCTGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGAt  >  2:964048/1‑140 (MQ=255)

CGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAACAGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGAT  >  NC_000913/3498376‑3498647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A