Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	161	50.8	1735388	98.6%	1711092	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	979,025	A→G	E900E (GAA→GAG)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	979,025	0	A	G	97.6%	137.9 / ‑4.5	41	E900E (GAA→GAG)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (18/22); total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAATGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACT  >  NC_000913/978892‑979161

aaaaTGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTc                                                                                                                                      <  1:582992/138‑1 (MQ=255)

aaaaTGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTc                                                                                                                                      >  2:582992/1‑138 (MQ=255)

  aaTGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGa                                                                                                                                  >  1:522661/1‑140 (MQ=255)

  aaTGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGa                                                                                                                                  >  1:461574/1‑140 (MQ=255)

    tGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATg                                                                                                                                <  1:243148/140‑1 (MQ=255)

    tGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATg                                                                                                                                <  2:461574/140‑1 (MQ=255)

      aTAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGaa                                                                                                                              <  2:820502/140‑1 (MQ=255)

      aTAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGaa                                                                                                                              >  1:406408/1‑140 (MQ=255)

      aTAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGaa                                                                                                                              <  1:745694/140‑1 (MQ=255)

                 cagcGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGc                                                                                                                   >  1:428413/1‑140 (MQ=255)

                    cGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                <  1:8518/140‑1 (MQ=255)

                    cGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                >  2:241069/1‑140 (MQ=255)

                    cGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                >  2:286724/1‑140 (MQ=255)

                    cGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                <  2:406408/140‑1 (MQ=255)

                               tGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTc                                                                                                     <  1:504473/140‑1 (MQ=255)

                                gAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                <  2:682483/128‑1 (MQ=255)

                                gAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCcgc                                                                                                                >  1:682483/1‑128 (MQ=255)

                                gAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGtt                                                                                                      <  2:513415/138‑1 (MQ=255)

                                gAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTACCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGtt                                                                                                      >  1:513415/1‑138 (MQ=255)

                                      gCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGtt                                                                                              >  2:155048/1‑140 (MQ=255)

                                                gCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCaa                                                                                    <  2:522661/140‑1 (MQ=255)

                                                  gTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACt                                                                                  >  1:212973/1‑140 (MQ=255)

                                                       ggCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCa                                                                                     <  1:373291/132‑1 (MQ=255)

                                                       ggCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCa                                                                                     >  2:373291/1‑132 (MQ=255)

                                                         cgcTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGaaa                                                                           >  1:255496/1‑140 (MQ=255)

                                                              aaCCACATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGa                                                                      >  2:293552/1‑140 (MQ=255)

                                                                            gcgTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCgg                                                        <  1:293552/140‑1 (MQ=255)

                                                                             cgTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGt                                                       >  1:634163/1‑140 (MQ=255)

                                                                                      cTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCagag                                              <  1:241069/140‑1 (MQ=255)

                                                                                      cTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCagag                                              <  2:212973/140‑1 (MQ=255)

                                                                                      cTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCagag                                              <  1:286724/140‑1 (MQ=255)

                                                                                      cTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCagag                                              <  2:428413/140‑1 (MQ=255)

                                                                                               gatgaCAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGa                                     <  1:725475/140‑1 (MQ=255)

                                                                                                 tgaCAGCCTGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAAc                                   <  2:634163/140‑1 (MQ=255)

                                                                                                         tGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGaa                           <  1:155048/140‑1 (MQ=255)

                                                                                                         tGGCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGaa                           <  1:852513/140‑1 (MQ=255)

                                                                                                         tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGaa                           <  1:329670/140‑1 (MQ=255)

                                                                                                          ggCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGaa                           <  2:600814/139‑1 (MQ=255)

                                                                                                          ggCGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGaa                           >  1:600814/1‑139 (MQ=255)

                                                                                                            cGGATCGCGTCGATGAAATCCGCGAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAg                        >  1:106056/1‑140 (MQ=255)

                                                                                                                                  cgAGCGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGAAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTAct  >  1:270782/1‑140 (MQ=255)

AAAATGATAACCAGCAGCAGCGTATTCAGTTTGAGCAGGCGAAAGAGGGCGTTACGGCGCTGAACCGCATTCTGCCGCGTCTCAACCTGTTGGCTGATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTCGGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACT  >  NC_000913/978892‑979161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A