Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	161	50.8	1735388	98.6%	1711092	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,450,841	G→A	A170V (GCG→GTG)	tynA ←	copper‑containing amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,450,841	0	G	A	98.0%	164.9 / ‑4.3	50	A170V (GCG→GTG)	tynA	copper‑containing amine oxidase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (25/24); total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

TACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCGCAGTTAGCGCA  >  NC_000913/1450704‑1450969

tACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCa                                                                                                                                <  1:257207/140‑1 (MQ=255)

 aCCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGAGATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACaa                                                                                                                               >  2:602583/1‑140 (MQ=255)

     tGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGc                                                                                                                           >  1:685614/1‑140 (MQ=255)

      gggCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGcc                                                                                                                          >  2:351694/1‑140 (MQ=255)

        gCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCa                                                                                                                        <  1:351694/140‑1 (MQ=255)

        gCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCa                                                                                                                        <  1:602583/140‑1 (MQ=255)

            cTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACa                                                                                                                    <  1:626292/140‑1 (MQ=255)

                      ttGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGccc                                                                                                                         >  2:88743/1‑125 (MQ=255)

                      ttGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGccc                                                                                                                         <  1:88743/125‑1 (MQ=255)

                         ccAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGAc                                                                                                                     >  2:631529/1‑126 (MQ=255)

                         ccAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGAc                                                                                                                     <  1:631529/126‑1 (MQ=255)

                         ccAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTg                                                                                                       >  1:805634/1‑140 (MQ=255)

                          cAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTgg                                                                                                      >  1:87832/1‑140 (MQ=255)

                            ggAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCCTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCg                                                                                                    <  2:805634/140‑1 (MQ=255)

                               gagCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTa                                                                                                 >  1:406438/1‑140 (MQ=255)

                                 gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGc                                                                                               <  2:87832/140‑1 (MQ=255)

                                  cAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCa                                                                                              >  2:175351/1‑140 (MQ=255)

                                         ttgttTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAt                                                                                       <  2:292696/140‑1 (MQ=255)

                                               tGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                                                                                 >  2:570737/1‑140 (MQ=255)

                                                gCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTa                                                                                <  2:866150/140‑1 (MQ=255)

                                                   gATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaaaa                                                                             <  2:406438/140‑1 (MQ=255)

                                                    aTCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAAc                                                                            >  2:535465/1‑140 (MQ=255)

                                                    aTCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACCTCGGCTTTGCGCGGCTGGTCAACCGCCTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAAc                                                                            >  2:699705/1‑140 (MQ=255)

                                                          caccGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTAt                                                                      <  2:470307/140‑1 (MQ=255)

                                                              gCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTggg                                                                  >  2:240330/1‑140 (MQ=255)

                                                               cTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                                                                                 >  1:390774/1‑124 (MQ=255)

                                                               cTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                                                                                 <  2:390774/124‑1 (MQ=255)

                                                               cTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGt                                                                 >  1:430666/1‑140 (MQ=255)

                                                               cTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGt                                                                 >  1:863853/1‑140 (MQ=255)

                                                               cTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGt                                                                 >  1:578278/1‑140 (MQ=255)

                                                                ttCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGtt                                                                >  2:338591/1‑140 (MQ=255)

                                                                  cGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTg                                                              <  1:455022/140‑1 (MQ=255)

                                                                    atgatATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGaa                                                            <  2:718902/140‑1 (MQ=255)

                                                                           gTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCcgcg                                                     <  2:685614/140‑1 (MQ=255)

                                                                              tGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGaa                                                  >  2:350468/1‑140 (MQ=255)

                                                                                      aGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAAc                                          >  1:768604/1‑140 (MQ=255)

                                                                                             tGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCa                                   <  2:373374/140‑1 (MQ=255)

                                                                                                cGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACg                                >  2:660910/1‑140 (MQ=255)

                                                                                                  tCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              <  1:338591/140‑1 (MQ=255)

                                                                                                  tCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              >  1:666544/1‑140 (MQ=255)

                                                                                                  tCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              <  2:578278/140‑1 (MQ=255)

                                                                                                  tCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              >  1:564674/1‑140 (MQ=255)

                                                                                                     gCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTg                           >  2:510666/1‑140 (MQ=255)

                                                                                                     gCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTg                           >  2:71925/1‑140 (MQ=255)

                                                                                                         tGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTa                       <  2:666544/140‑1 (MQ=255)

                                                                                                                   gTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              >  2:367672/1‑123 (MQ=255)

                                                                                                                   gTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGc                              <  1:367672/123‑1 (MQ=255)

                                                                                                                   gTCAACCGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCg             <  1:570737/140‑1 (MQ=255)

                                                                                                                        ccGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCGCAGtt        <  1:535465/140‑1 (MQ=255)

                                                                                                                         cGGTTTGTTTTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCGCAGTTa       <  2:564674/140‑1 (MQ=255)

                                                                                                                              tgtttTCCAGCACAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCGCAGTTAGCGCa  <  1:510666/140‑1 (MQ=255)

TACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGTTTGAAGTCCGCGGAAGCTTTAACAATTTCAACGGCCTGTTTAATTTCGTCCGCAGTTAGCGCA  >  NC_000913/1450704‑1450969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A