Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	132	43.0	1464274	99.1%	1451095	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,564	A→G	W164R (TGG→CGG)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,564	0	A	G	100.0%	126.4 / NA	37	W164R (TGG→CGG)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/23); total (14/23)

TCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCACTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGC  >  NC_000913/1019428‑1019695

tCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTg                                                                                                                                  <  1:179990/140‑1 (MQ=255)

 ccGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTgg                                                                                                                                 >  1:130091/1‑140 (MQ=255)

  cGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGt                                                                                                                                >  2:312472/1‑140 (MQ=255)

    gTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTAt                                                                                                                              <  1:341939/140‑1 (MQ=255)

                gAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTa                                                                                                                  <  2:601995/140‑1 (MQ=255)

                  gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:701686/140‑1 (MQ=255)

                  gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:313490/140‑1 (MQ=255)

                  gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:229380/140‑1 (MQ=255)

                       aGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGAttt                                                                                                           <  1:312472/140‑1 (MQ=255)

                            ctactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGa                                                                                                      >  1:161301/1‑140 (MQ=255)

                             tactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAg                                                                                                     <  2:655537/140‑1 (MQ=255)

                             tactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAg                                                                                                     <  1:302871/140‑1 (MQ=255)

                               ctGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTg                                                                                                   <  2:266688/140‑1 (MQ=255)

                                        ctTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTAc                                                                                          <  2:292162/140‑1 (MQ=255)

                                                 gACCGAAACGGTAGGAAACCCCCAGGCTCAGCAGGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCg                                                                                 <  2:111315/140‑1 (MQ=255)

                                                  aCCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGc                                                                                >  1:702455/1‑140 (MQ=255)

                                                      aaaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGc                                                                            <  2:130091/140‑1 (MQ=255)

                                                       aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTa                                                                                           >  1:510781/1‑124 (MQ=255)

                                                       aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTa                                                                                           <  2:510781/124‑1 (MQ=255)

                                                       aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCg                                                                           >  2:104708/1‑140 (MQ=255)

                                                                       cAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGc                                                           <  1:625718/140‑1 (MQ=255)

                                                                        aGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGcc                                                          >  2:193311/1‑140 (MQ=255)

                                                                         ggctgcgCATGCCGTTGTCCGGACGAGTGCCGAGGGTGTGTGCGTCCCCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTGGCGATTTCAGGCGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCg                                                         <  1:209065/134‑1 (MQ=255)

                                                                         ggCTCAGCATGCCGTTGGCCGGGCGGGGGCCGGTGGGTGGGGGGGCACCGATGTTGTTTGTCCGCTTGTTTTCCAGGCGGGGAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCg                                                         <  2:442171/140‑1 (MQ=255)

                                                                               gCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCg                                                   <  1:104708/140‑1 (MQ=255)

                                                                                cATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGt                                                  >  2:229476/1‑140 (MQ=255)

                                                                                 aTGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTg                                                 <  2:161301/140‑1 (MQ=255)

                                                                                   gCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGt                                               <  1:422709/140‑1 (MQ=255)

                                                                                      gTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGtttt                                            <  1:229476/140‑1 (MQ=255)

                                                                                        tGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTa                                          >  2:24379/1‑140 (MQ=255)

                                                                                                              gtgtgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAgtgt                    >  2:263851/1‑140 (MQ=255)

                                                                                                              gtgtgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAgtgt                    >  2:415330/1‑140 (MQ=255)

                                                                                                                 tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 >  2:389697/1‑140 (MQ=255)

                                                                                                                 tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 <  1:661511/140‑1 (MQ=255)

                                                                                                                 tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 <  1:370226/140‑1 (MQ=255)

                                                                                                                                gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATg   <  2:231640/139‑1 (MQ=255)

                                                                                                                                gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATg   >  1:231640/1‑139 (MQ=255)

                                                                                                                                gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGc  >  2:8884/1‑140 (MQ=255)

TCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCACTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGC  >  NC_000913/1019428‑1019695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A