Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,000	A→G	E83E (GAA→GAG)	ycbJ →	putative phosphotransferase YcbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,000	0	A	G	100.0%	151.1 / NA	43	E83E (GAA→GAG)	ycbJ	putative phosphotransferase YcbJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/17); total (26/17)

ACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAACACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCA  >  NC_000913/971867‑972131

aCCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCAcccc                                                                                                                               >  2:909304/1‑140 (MQ=255)

  ccAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCgg                                                                                                                             <  2:842809/140‑1 (MQ=255)

      tGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGcccc                                                                                                                         >  2:408737/1‑140 (MQ=255)

       gCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCg                                                                                                                        <  1:916717/140‑1 (MQ=255)

             aaTGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAATCCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTcc                                                                                                                  <  1:909304/140‑1 (MQ=255)

             aaTGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTcc                                                                                                                  <  1:626767/140‑1 (MQ=255)

                 gCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCt                                                                                                              >  2:860762/1‑140 (MQ=255)

                                    ccGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCgtggg                                                                                           >  2:103568/1‑139 (MQ=255)

                                                   aaCTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCa                                                                            <  1:408737/140‑1 (MQ=255)

                                                    aCTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCAc                                                                           <  1:212527/140‑1 (MQ=255)

                                                             gAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGaa                                                                  >  1:119159/1‑140 (MQ=255)

                                                                  ccaccaTGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCa                                                             >  2:85034/1‑140 (MQ=255)

                                                                       aTGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACg                                                        >  2:343055/1‑140 (MQ=255)

                                                                        tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGc                                                                       <  2:574599/124‑1 (MQ=255)

                                                                        tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGc                                                                       >  1:574599/1‑124 (MQ=255)

                                                                        tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGc                                                       >  2:444639/1‑140 (MQ=255)

                                                                            ggCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTggg                                                   <  1:343055/140‑1 (MQ=255)

                                                                             gCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGa                                                  >  1:762671/1‑140 (MQ=255)

                                                                                agaagTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaaca                                               <  2:762671/140‑1 (MQ=255)

                                                                                        gACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAg                                       >  2:333366/1‑140 (MQ=255)

                                                                                         aCTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGa                                      >  1:505529/1‑140 (MQ=255)

                                                                                            gTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaacaac                                             >  2:806198/1‑130 (MQ=255)

                                                                                            gTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaacaac                                             <  1:806198/130‑1 (MQ=255)

                                                                                             tCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCaa                                  >  2:769469/1‑140 (MQ=255)

                                                                                              ccGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCaaa                                 >  1:316982/1‑140 (MQ=255)

                                                                                                       gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        <  1:444639/140‑1 (MQ=255)

                                                                                                       gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        >  2:800413/1‑140 (MQ=255)

                                                                                                       gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        >  2:14360/1‑140 (MQ=255)

                                                                                                            tttATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTa                   <  2:99648/140‑1 (MQ=255)

                                                                                                            tttATGGTGTCATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTa                   >  1:869789/1‑140 (MQ=255)

                                                                                                             ttATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTAc                  <  1:860762/140‑1 (MQ=255)

                                                                                                               aTGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActg                >  1:51519/1‑140 (MQ=255)

                                                                                                                    gtgATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctg           >  1:92651/1‑140 (MQ=255)

                                                                                                                      gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:132541/1‑140 (MQ=255)

                                                                                                                      gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  1:769469/140‑1 (MQ=255)

                                                                                                                      gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:591041/1‑140 (MQ=255)

                                                                                                                      gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  1:333366/140‑1 (MQ=255)

                                                                                                                       atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        >  2:681949/1‑140 (MQ=255)

                                                                                                                       atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        >  1:912047/1‑140 (MQ=255)

                                                                                                                       atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        <  1:85034/140‑1 (MQ=255)

                                                                                                                             cATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCa  >  2:867201/1‑140 (MQ=255)

                                                                                                                               tGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  2:766518/131‑1 (MQ=255)

                                                                                                                               tGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:766518/1‑131 (MQ=255)

ACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAACACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCA  >  NC_000913/971867‑972131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A