Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	148	57.0	1973990	96.6%	1906874	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,248,432	A→G	L228P (CTG→CCG)	dhaM ←	dihydroxyacetone kinase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,248,432	0	A	G	100.0%	148.5 / NA	43	L228P (CTG→CCG)	dhaM	dihydroxyacetone kinase subunit M
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/24); total (19/24)

TTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATC  >  NC_000913/1248297‑1248559

tttGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGc                                                                                                                             <  1:368147/140‑1 (MQ=255)

     cTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTCCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCtt                                                                                                                        <  1:951153/140‑1 (MQ=255)

             tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:88462/1‑140 (MQ=255)

             tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:139809/1‑140 (MQ=255)

             tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:827007/1‑140 (MQ=255)

                       tGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                                                                      <  2:108421/140‑1 (MQ=255)

                       tGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                                                                      <  1:319770/140‑1 (MQ=255)

                         gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGTAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCa                                                                                                    >  1:324649/1‑140 (MQ=255)

                         gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGcc                                                                                                            >  1:412453/1‑132 (MQ=255)

                         gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGcc                                                                                                            <  2:412453/132‑1 (MQ=255)

                          ttGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCaa                                                                                                   >  2:652781/1‑140 (MQ=255)

                                 taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  1:652781/140‑1 (MQ=255)

                                 taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  2:722370/140‑1 (MQ=255)

                                 taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  1:934288/140‑1 (MQ=255)

                                   aaaGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCa                                                                                          >  1:326936/1‑140 (MQ=255)

                                         tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt                                                                                    >  1:563937/1‑140 (MQ=255)

                                           tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg                                                                                       >  1:36314/1‑135 (MQ=255)

                                           tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg                                                                                       <  2:36314/135‑1 (MQ=255)

                                           tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCg                                                                                  <  1:563423/140‑1 (MQ=255)

                                           tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAACCAGGCGCAGCg                                                                                       >  2:99460/1‑135 (MQ=255)

                                           tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAACCAGGCGCAGCg                                                                                       <  1:99460/135‑1 (MQ=255)

                                            aCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGt                                                                                 >  1:736123/1‑140 (MQ=255)

                                                  aaCAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGc                                                                           <  2:563937/140‑1 (MQ=255)

                                                       gCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACt                                                                      >  1:663317/1‑140 (MQ=255)

                                                         ggAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg                                                                    <  1:88462/140‑1 (MQ=255)

                                                                 aCGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta                                                               >  1:738767/1‑137 (MQ=255)

                                                                 aCGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta                                                               <  2:738767/137‑1 (MQ=255)

                                                                     agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  2:518093/140‑1 (MQ=255)

                                                                     agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  2:5427/140‑1 (MQ=255)

                                                                     agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  1:240159/140‑1 (MQ=255)

                                                                       agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCt                                                      <  1:139809/140‑1 (MQ=255)

                                                                              ggAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAAt                                               <  2:154749/140‑1 (MQ=255)

                                                                                 gCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAct                                            >  2:215114/1‑140 (MQ=255)

                                                                                 gCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAct                                            >  2:917756/1‑140 (MQ=255)

                                                                                      ttCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTg                                       <  2:140273/140‑1 (MQ=255)

                                                                                           ccGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGtgtg                                  >  1:716755/1‑140 (MQ=255)

                                                                                               ttCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGc                              >  2:316509/1‑140 (MQ=255)

                                                                                                tCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCa                             >  1:459698/1‑140 (MQ=255)

                                                                                                             cTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGtttttt                <  2:736123/140‑1 (MQ=255)

                                                                                                               tCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTc              <  2:716755/140‑1 (MQ=255)

                                                                                                                  gccagcTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAg           <  2:663317/140‑1 (MQ=255)

                                                                                                                          aCGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCTAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAatatat   <  2:684188/140‑1 (MQ=255)

                                                                                                                           cGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATc  >  2:209122/1‑140 (MQ=255)

TTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATC  >  NC_000913/1248297‑1248559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A