Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	95	36.3	1231394	98.7%	1215385	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	877,873	A→G	F55L (TTT→CTT)	rimO ←	ribosomal protein S12 methylthiotransferase RimO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	877,873	0	A	G	100.0%	146.2 / NA	40	F55L (TTT→CTT)	rimO	ribosomal protein S12 methylthiotransferase RimO
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/20); total (20/20)

TTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGATT  >  NC_000913/877738‑878009

tttCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCg                                                                                                                                      <  2:330266/140‑1 (MQ=255)

tttCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCg                                                                                                                                      <  1:317419/140‑1 (MQ=255)

            tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  2:173039/1‑140 (MQ=255)

            tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  1:486102/1‑140 (MQ=255)

            tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  1:442100/1‑140 (MQ=255)

                           ggATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCa                                                                                                           <  2:459687/140‑1 (MQ=255)

                           ggATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCa                                                                                                           <  1:349328/140‑1 (MQ=255)

                                 gatctTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCa                                                                                                     <  1:260394/140‑1 (MQ=255)

                                      tcttTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCt                                                                                                <  2:126204/140‑1 (MQ=255)

                                         tttGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCgg                                                                                             >  1:167305/1‑140 (MQ=255)

                                          ttGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGt                                                                                            >  2:511684/1‑140 (MQ=255)

                                             cccccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTAcc                                                                                         <  1:376637/140‑1 (MQ=255)

                                             cccccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTAcc                                                                                         >  1:175569/1‑140 (MQ=255)

                                               cccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCAc                                                                                       >  1:265893/1‑140 (MQ=255)

                                                        ccGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAAcc                                                                              >  2:315977/1‑140 (MQ=255)

                                                        ccGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAAcc                                                                              >  1:585564/1‑140 (MQ=255)

                                                         cGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCt                                                                             <  2:265893/140‑1 (MQ=255)

                                                            tCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTATCACGTCATAACCTTCa                                                                          >  1:255155/1‑140 (MQ=255)

                                                                      cTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGtt                                                                <  1:315977/140‑1 (MQ=255)

                                                                         gCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCgg                                                             <  1:173039/140‑1 (MQ=255)

                                                                                 cattcaACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATa                                                     <  1:511684/140‑1 (MQ=255)

                                                                                     caACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGct                                                 <  2:442100/140‑1 (MQ=255)

                                                                                                  aTGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAg                                    >  2:94654/1‑140 (MQ=255)

                                                                                                  aTGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAg                                    >  2:62102/1‑140 (MQ=255)

                                                                                                       ttCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAACTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               >  2:472970/1‑140 (MQ=255)

                                                                                                        tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               <  1:332054/139‑1 (MQ=255)

                                                                                                        tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               >  2:332054/1‑139 (MQ=255)

                                                                                                        tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtt                                 <  2:591949/137‑1 (MQ=255)

                                                                                                        tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtt                                 >  1:591949/1‑137 (MQ=255)

                                                                                                            gTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          <  2:486102/140‑1 (MQ=255)

                                                                                                            gTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAACACGCTCTGAATCAACAAGGTTTTTCGGa                          >  2:35544/1‑140 (MQ=255)

                                                                                                             tGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGAc                         >  1:425640/1‑140 (MQ=255)

                                                                                                                ttCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGCGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGc                      <  2:585564/140‑1 (MQ=255)

                                                                                                                 tCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGcc                     <  2:167305/140‑1 (MQ=255)

                                                                                                                           cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          >  1:65908/1‑125 (MQ=255)

                                                                                                                           cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          <  2:65908/125‑1 (MQ=255)

                                                                                                                           cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACa           >  2:113374/1‑140 (MQ=255)

                                                                                                                           cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACa           >  1:465067/1‑140 (MQ=255)

                                                                                                                               gTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGc       <  2:66200/140‑1 (MQ=255)

                                                                                                                                    tAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTATCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTAAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGAtt  <  2:255155/140‑1 (MQ=255)

TTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGATT  >  NC_000913/877738‑878009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A