Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	95	36.3	1231394	98.7%	1215385	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,435	A→G	Y210C (TAC→TGC)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,435	0	A	G	100.0%	99.2 / NA	28	Y210C (TAC→TGC)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/16); total (12/16)

AAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG  >  NC_000913/1002308‑1002565

aaaaTATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTAc                                                                                                                        <  2:565713/140‑1 (MQ=255)

   atatTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGAt                                                                                                                     <  1:54927/140‑1 (MQ=255)

      ttGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCaa                                                                                                                  <  2:324123/140‑1 (MQ=255)

               ttcgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTgg                                                                                                         >  2:399157/1‑140 (MQ=255)

                 cgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCt                                                                                                       <  2:543196/140‑1 (MQ=255)

                 cgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGCACACCGGTTTTCGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCt                                                                                                       >  1:474646/1‑140 (MQ=255)

                       cTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGtt                                                                                                 <  1:170202/140‑1 (MQ=255)

                                    cctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCgg                                                                                    <  1:51559/140‑1 (MQ=255)

                                    cctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCgg                                                                                    <  1:188613/140‑1 (MQ=255)

                                     ctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGga                                                                                   <  1:399157/140‑1 (MQ=255)

                                     ctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGga                                                                                   <  2:304070/140‑1 (MQ=255)

                                        ctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGgagag                                                                                <  1:544826/140‑1 (MQ=255)

                                          aTGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGAc                                                                              >  1:295511/1‑140 (MQ=255)

                                               gctggcgcGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTg                                                                         >  2:418328/1‑140 (MQ=255)

                                                              tGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTg                                                          >  1:380021/1‑140 (MQ=255)

                                                                   gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGt                                                                     <  1:435372/124‑1 (MQ=255)

                                                                   gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGt                                                                     >  2:435372/1‑124 (MQ=255)

                                                                   gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTc                                                     >  1:30902/1‑140 (MQ=255)

                                                                   gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTc                                                     >  2:124724/1‑140 (MQ=255)

                                                                       caacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCg                                                 >  1:557103/1‑140 (MQ=255)

                                                                             aCCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCa                                           >  1:74483/1‑140 (MQ=255)

                                                                                           ccTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTa                             <  2:295511/140‑1 (MQ=255)

                                                                                               cACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGc                         >  2:238137/1‑140 (MQ=255)

                                                                                                aCGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCa                        <  2:380021/140‑1 (MQ=255)

                                                                                                   aCTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                     >  1:464530/1‑140 (MQ=255)

                                                                                                   aCTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                     <  1:238137/140‑1 (MQ=255)

                                                                                                     tACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                   <  2:464530/140‑1 (MQ=255)

                                                                                                     tACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                   <  2:474646/140‑1 (MQ=255)

                                                                                                                      tAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACg  <  2:74483/140‑1 (MQ=255)

AAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG  >  NC_000913/1002308‑1002565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A