Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
157 |
47.3 |
1607120 |
99.4% |
1597477 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
865,679 |
T→A |
D229V (GAT→GTT) |
moeA ← |
molybdopterin molybdotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 865,679 | 0 | T | A | 100.0%
| 84.4
/ NA
| 25 | D229V (GAT→GTT) | moeA | molybdopterin molybdotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/13); total (12/13) |
AGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTA > NC_000913/865545‑865813
|
aGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAacgtcg > 1:776344/1‑140 (MQ=255)
ggCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGa < 1:713214/140‑1 (MQ=255)
gCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGAt < 2:776344/140‑1 (MQ=255)
aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAAt > 2:147249/1‑140 (MQ=255)
ccccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTa < 2:455009/140‑1 (MQ=255)
cccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTaa > 2:28975/1‑140 (MQ=255)
gTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATcc > 1:510352/1‑140 (MQ=255)
aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGtt < 2:29888/137‑1 (MQ=255)
aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGtt > 1:29888/1‑137 (MQ=255)
ccGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTagc < 1:783293/140‑3 (MQ=255)
cTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCa > 2:432146/1‑140 (MQ=255)
cccACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGgtg > 2:168000/1‑140 (MQ=255)
cccACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGgtg < 1:305802/140‑1 (MQ=255)
aaaCACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCg < 1:147249/140‑1 (MQ=255)
aaCACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCga > 1:734427/1‑140 (MQ=255)
acacCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCgag < 2:510352/140‑1 (MQ=255)
ccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGtt > 2:710626/1‑140 (MQ=255)
gATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGt > 2:306707/1‑140 (MQ=255)
ccTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCg > 2:168293/1‑140 (MQ=255)
ggCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGccc < 1:710626/140‑1 (MQ=255)
ttCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGAc < 1:469972/140‑1 (MQ=255)
tGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACt > 2:130242/1‑140 (MQ=255)
gcgcgcAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAg > 1:55784/1‑140 (MQ=255)
ggCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCAc < 1:168000/140‑1 (MQ=255)
cATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCg < 1:181662/140‑1 (MQ=255)
ggggAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTa < 2:55784/140‑1 (MQ=255)
|
AGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTA > NC_000913/865545‑865813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
