Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 157 47.3 1607120 99.4% 1597477 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,483,525 T→C G155G (GGT→GGC)  hrpA → ATP‑dependent RNA helicase HrpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,483,5250TC100.0% 190.1 / NA 54G155G (GGT→GGC) hrpAATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

TGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAA  >  NC_000913/1483400‑1483654
                                                                                                                             |                                                                                                                                 
tGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGttt                                                                                                                     <  2:471873/140‑1 (MQ=255)
       aGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGAt                                                                                                              >  1:133261/1‑140 (MQ=255)
       aGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGAt                                                                                                              >  2:625715/1‑140 (MQ=255)
         cTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGa                                                                                                               >  2:485595/1‑137 (MQ=255)
         cTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGa                                                                                                               <  1:485595/137‑1 (MQ=255)
           ggggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACg                                                                                                          <  1:71001/140‑1 (MQ=255)
            gggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGt                                                                                                         <  2:612134/140‑1 (MQ=255)
            gggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGt                                                                                                         <  2:550784/140‑1 (MQ=255)
                      ttAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAac                                                                                               >  2:90378/1‑140 (MQ=255)
                            gACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATg                                                                                         >  1:166404/1‑140 (MQ=255)
                               tGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGAt                                                                                          >  1:389504/1‑136 (MQ=255)
                               tGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGAt                                                                                          <  2:389504/136‑1 (MQ=255)
                                gATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCa                                                                                     >  1:626606/1‑140 (MQ=255)
                                 aTCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCaa                                                                                    >  1:259322/1‑140 (MQ=255)
                                 aTCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCaa                                                                                    >  1:467924/1‑140 (MQ=255)
                                          aCCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           <  1:625715/140‑1 (MQ=255)
                                           cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATgg                                                                                        <  2:781698/126‑1 (MQ=255)
                                           cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATgg                                                                                        >  1:781698/1‑126 (MQ=255)
                                           cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAcc                                                                          >  2:40984/1‑140 (MQ=255)
                                             cAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGa                                                                        <  2:688582/140‑1 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           >  1:371796/1‑134 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           <  2:371796/134‑1 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:717189/1‑140 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:273099/1‑140 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  2:439208/1‑140 (MQ=255)
                                                ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:529243/1‑140 (MQ=255)
                                                   cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  >  2:335142/1‑140 (MQ=255)
                                                   cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  <  2:598263/140‑1 (MQ=255)
                                                   cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  >  1:298544/1‑140 (MQ=255)
                                                     tcgtcTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATcc                                                                >  2:627323/1‑140 (MQ=255)
                                                       gtcTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCctg                                                              <  1:40984/140‑1 (MQ=255)
                                                             cggcAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTCTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCg                                                        <  2:717189/140‑1 (MQ=255)
                                                              ggcAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCgg                                                       <  1:627323/140‑1 (MQ=255)
                                                                 aaGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGTGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGgaga                                                    <  2:133261/140‑1 (MQ=255)
                                                                           gCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGa                                          >  2:10702/1‑140 (MQ=255)
                                                                              aaCCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCg                                       <  1:335142/140‑1 (MQ=255)
                                                                              aaCCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCg                                       <  1:534411/140‑1 (MQ=255)
                                                                                   tATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCctgc                                  <  2:467924/140‑1 (MQ=255)
                                                                                      tGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGa                               <  2:166404/140‑1 (MQ=255)
                                                                                      tGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGa                               <  2:259322/140‑1 (MQ=255)
                                                                                        cGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATg                             <  1:302410/140‑1 (MQ=255)
                                                                                        cGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATg                             >  2:302410/1‑140 (MQ=255)
                                                                                           aaGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAg                          <  2:407250/140‑1 (MQ=255)
                                                                                             gagCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTa                        >  1:28514/1‑140 (MQ=255)
                                                                                             gagCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTa                        <  2:28514/140‑1 (MQ=255)
                                                                                                cTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGa                     >  1:714978/1‑140 (MQ=255)
                                                                                                cTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGa                     <  1:90378/140‑1 (MQ=255)
                                                                                                 tGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGac                    <  2:445708/140‑1 (MQ=255)
                                                                                                  gAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  2:529243/140‑1 (MQ=255)
                                                                                                  gAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  2:273099/140‑1 (MQ=255)
                                                                                                     aaCGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTa                <  1:10702/140‑1 (MQ=255)
                                                                                                            ccGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  1:282545/130‑1 (MQ=255)
                                                                                                            ccGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   >  2:282545/1‑130 (MQ=255)
                                                                                                                   gTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGaa  <  1:439208/140‑1 (MQ=255)
                                                                                                                             |                                                                                                                                 
TGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAA  >  NC_000913/1483400‑1483654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A