Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
118 |
32.6 |
1094092 |
99.3% |
1086433 |
139.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,263,889 |
T→C |
V59A (GTT→GCT) |
hemA → |
glutamyl‑tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,263,889 | 0 | T | C | 100.0%
| 82.3
/ NA
| 24 | V59A (GTT→GCT) | hemA | glutamyl‑tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/14); total (10/14) |
GCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTG > NC_000913/1263764‑1264023
|
gCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACa > 1:45658/1‑140 (MQ=255)
tgtATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAag > 1:283081/1‑140 (MQ=255)
tATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAagag > 2:454099/1‑140 (MQ=255)
ttttCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGtt < 2:152032/140‑1 (MQ=255)
gCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCttt < 1:260784/140‑1 (MQ=255)
gCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCttt < 1:454099/140‑1 (MQ=255)
aTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGAt > 1:369170/1‑140 (MQ=255)
cAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTa > 2:142474/1‑140 (MQ=255)
cTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACaa < 1:517083/140‑1 (MQ=255)
ttGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAAt < 2:283081/140‑1 (MQ=255)
cAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTa < 2:82553/140‑1 (MQ=255)
aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTaa < 1:75136/140‑1 (MQ=255)
aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTCATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTGTCACAATCTTaa < 2:473084/140‑1 (MQ=255)
cAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGTGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCct < 2:236315/140‑1 (MQ=255)
aGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCctc > 2:179287/1‑140 (MQ=255)
cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc > 1:59716/1‑140 (MQ=255)
cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc > 1:337826/1‑140 (MQ=255)
cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc > 1:141886/1‑140 (MQ=255)
tggtgCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGGTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCAt < 1:151675/140‑1 (MQ=255)
aaCCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGt < 2:246108/140‑1 (MQ=255)
ccGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTa < 2:141886/140‑1 (MQ=255)
ccGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTa < 2:369170/140‑1 (MQ=255)
aCTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTaa < 2:45658/140‑1 (MQ=255)
tAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTg > 2:516173/1‑140 (MQ=255)
|
GCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTG > NC_000913/1263764‑1264023
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
