Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I1 R1 108 324.7 15372274 99.7% 15326157 98.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,0101.C77.6% 837.9 / 256.5 263coding (193/2019 nt)hyfBhydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base . (32/27);  new base C (95/109);  total (127/136)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 281 total aligned reads displayed.

TATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTA  >  NC_000913/2601910‑2602110
                                                                                                     |                                                                                                     
tATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                        >  2:4237145/1‑101 (MQ=255)
    aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccc                                                                                                   >  2:4237149/1‑101 (MQ=255)
        tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccc‑cc                                                                                              <  1:4236823/101‑2 (MQ=255)
        tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccc‑cc                                                                                              >  1:4237153/1‑100 (MQ=255)
          tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Cctt                                                                                            >  1:4237156/1‑101 (MQ=255)
           ccGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Ccttt                                                                                           <  2:4237004/101‑1 (MQ=255)
           ccGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Ccttt                                                                                           >  2:4237157/1‑101 (MQ=255)
            cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCACtttt                                                                                           <  1:4236722/101‑1 (MQ=255)
            cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Cctttt                                                                                          <  2:4236619/101‑2 (MQ=255)
            cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Cctttt                                                                                          <  2:4236548/101‑2 (MQ=255)
              gCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGa                                                                                         <  2:4236693/101‑1 (MQ=255)
              gCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGa                                                                                         <  1:4236631/101‑1 (MQ=255)
               cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGaa                                                                                        >  1:4237162/1‑101 (MQ=255)
               cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Cctttt                                                                                          >  2:4237163/1‑97 (MQ=255)
                aTCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAAt                                                                                       <  2:4236803/101‑1 (MQ=255)
                aTCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAAt                                                                                       <  2:4236487/101‑1 (MQ=255)
                  cacaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAAttt                                                                                     >  2:4237167/1‑101 (MQ=255)
                  cacaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAAttt                                                                                     <  2:4236579/101‑1 (MQ=255)
                    caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGc                                                                                   <  1:4236468/101‑1 (MQ=255)
                     aTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGcc                                                                                  >  1:4237170/1‑101 (MQ=255)
                      tCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCg                                                                                 <  2:4236982/101‑1 (MQ=255)
                        gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGAt                                                                               >  2:4237174/1‑101 (MQ=255)
                        gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGAt                                                                               <  1:4236742/101‑1 (MQ=255)
                        gCTGGTGGGCGGCGCGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGAt                                                                               <  1:4236454/101‑1 (MQ=255)
                          tggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGAttt                                                                             <  1:4236994/101‑1 (MQ=255)
                            gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTaa                                                                           <  2:4236747/101‑1 (MQ=255)
                            gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTaa                                                                           >  2:4237177/1‑101 (MQ=255)
                              gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAAcc                                                                         <  1:4236765/101‑1 (MQ=255)
                              gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAAccc                                                                        >  1:4237179/1‑101 (MQ=255)
                                  ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                     <  1:4236750/101‑1 (MQ=255)
                                  ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                     >  2:4237182/1‑101 (MQ=255)
                                  ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                     >  2:4237184/1‑101 (MQ=255)
                                  ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                     <  2:4236884/101‑1 (MQ=255)
                                   gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCg                                                                    >  1:4237186/1‑101 (MQ=255)
                                    cgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGa                                                                   >  1:4237189/1‑101 (MQ=255)
                                    cgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGa                                                                   <  1:4236572/101‑1 (MQ=255)
                                     gTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                  >  2:4237192/1‑101 (MQ=255)
                                      tGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAAt                                                                 <  1:4236894/101‑1 (MQ=255)
                                       gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATg                                                                >  1:4237195/1‑101 (MQ=255)
                                       gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATg                                                                <  1:4236675/101‑1 (MQ=255)
                                       gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATg                                                                <  2:4236974/101‑1 (MQ=255)
                                       gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATgg                                                               <  1:4236868/101‑1 (MQ=255)
                                         ttGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                              <  2:4236859/101‑1 (MQ=255)
                                           ggCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATa                                                            >  2:4237199/1‑101 (MQ=255)
                                             catcatCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGc                                                          >  1:4237200/1‑101 (MQ=255)
                                              atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                        <  2:4236725/101‑1 (MQ=255)
                                               tcatcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                        <  1:4236700/101‑1 (MQ=255)
                                                 atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                      >  1:4237203/1‑101 (MQ=255)
                                                   caGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCg                                                    >  1:4237206/1‑101 (MQ=255)
                                                      cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCa                                                 >  2:4237211/1‑101 (MQ=255)
                                                       ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAt                                                <  1:4236796/101‑1 (MQ=255)
                                                        gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAtt                                               >  1:4237215/1‑101 (MQ=255)
                                                        gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                              <  2:4237069/101‑1 (MQ=255)
                                                        gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                              >  2:4237214/1‑101 (MQ=255)
                                                         aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                              <  2:4236658/101‑1 (MQ=255)
                                                         aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                              <  1:4236846/101‑1 (MQ=255)
                                                         aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                              <  2:4236880/101‑1 (MQ=255)
                                                           ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATg                                           <  2:4236888/101‑1 (MQ=255)
                                                            tACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATg                                           <  1:4237070/101‑1 (MQ=255)
                                                            tACGCAATTACATGCTGGTGT‑ACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTTAATTTGcc                                                                                  >  2:4237218/1‑61 (MQ=255)
                                                             aCGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATgg                                          >  1:4237219/1‑101 (MQ=255)
                                                               gCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                        >  1:4237224/1‑101 (MQ=255)
                                                                cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                       >  2:4237226/1‑101 (MQ=255)
                                                                cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                       <  1:4236654/101‑1 (MQ=255)
                                                                cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCt                                      <  1:4236808/101‑1 (MQ=255)
                                                                 aaTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCt                                      <  1:4236708/101‑1 (MQ=255)
                                                                 aaTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTg                                     >  1:4237229/1‑101 (MQ=255)
                                                                   ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                    <  2:4236766/101‑1 (MQ=255)
                                                                    tACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                   <  2:4236840/101‑1 (MQ=255)
                                                                    tACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                   <  2:4236948/101‑1 (MQ=255)
                                                                     aCATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCg                                                                    >  2:4237233/1‑67 (MQ=255)
                                                                      cATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTa                                 >  1:4237235/1‑101 (MQ=255)
                                                                        tGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                               >  1:4237239/1‑101 (MQ=255)
                                                                          cTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑Cctttt                                                                                          >  2:4237240/1‑38 (MQ=255)
                                                                            ggTGTAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCt                           <  1:4236932/101‑1 (MQ=255)
                                                                             gtgtAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCtt                          >  1:4237245/1‑101 (MQ=255)
                                                                              tgtAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTg                         <  2:4236730/101‑1 (MQ=255)
                                                                              tgtAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTg                         >  2:4237246/1‑101 (MQ=255)
                                                                              tgtAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGc                        <  2:4236819/101‑1 (MQ=255)
                                                                                tAACTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCt                       <  1:4236957/101‑1 (MQ=255)
                                                                                tAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtg                      >  1:4237248/1‑101 (MQ=255)
                                                                                  aCTTTAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtgg                     <  1:4236773/101‑1 (MQ=255)
                                                                                  aCTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggt                    >  1:4237251/1‑101 (MQ=255)
                                                                                   cTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                   >  2:4237255/1‑101 (MQ=255)
                                                                                    tttAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGcc                                                                                  >  1:4237262/1‑38 (MQ=255)
                                                                                    tttAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATgg                                                               >  2:4237261/1‑57 (MQ=255)
                                                                                    tttAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                   >  2:4237258/1‑101 (MQ=255)
                                                                                    tttAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                   <  1:4236826/101‑1 (MQ=255)
                                                                                      tAGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggt                 >  1:4237265/1‑101 (MQ=255)
                                                                                       aGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCtt                          >  1:4237270/1‑91 (MQ=255)
                                                                                       aGTCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtg                <  1:4237167/101‑1 (MQ=255)
                                                                                         tCGCCCGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt              >  2:4237272/1‑101 (MQ=255)
                                                                                         tCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg               >  1:4237274/1‑99 (MQ=255)
                                                                                            cccGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctc                                                       <  2:4236911/57‑1 (MQ=255)
                                                                                              cGTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt         <  2:4236791/101‑1 (MQ=255)
                                                                                              cGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc        >  1:4237277/1‑101 (MQ=255)
                                                                                               gTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAAccc                                                                        >  2:4237279/1‑37 (MQ=37)
                                                                                               gTTTTGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc        <  2:4236929/101‑1 (MQ=255)
                                                                                                ttttGCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct       <  2:4236849/101‑1 (MQ=255)
                                                                                                  ttG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc    >  1:4237286/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
TATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTA  >  NC_000913/2601910‑2602110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A