Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	115	276.2	7960526	99.2%	7896841	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,130	A→T	intergenic (‑244/‑57)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,130	0	A	T	99.5%	701.0 / NA	197	intergenic (‑244/‑57)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (112/84); minor base C (1/0); total (113/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑T‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGA  >  CP006881/3722985‑3723276

gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                                                                                                 >  1:3953979/1‑150 (MQ=255)

 ccTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                                                                                                                                <  1:3806933/150‑1 (MQ=255)

   tGCTTTCCCAGTCCCAGTCCCCCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑GTT‑T‑TTT‑TAGCTAAATAGTGTGATATAACATGTAGACAAAACTTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                                              <  1:2232042/150‑1 (MQ=255)

      tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                                                                                                           >  1:2874309/1‑150 (MQ=255)

                acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCta                                                                                                                                 >  1:3076064/1‑150 (MQ=255)

                acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCta                                                                                                                                 <  1:1425111/150‑1 (MQ=255)

                 caATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑A‑TAT‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtat                                                                                                                                >  1:2385994/1‑150 (MQ=255)

                 caATCCCGCTTATTCAGAGTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtat                                                                                                                                <  1:1651375/150‑1 (MQ=255)

                   aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                              >  2:870693/1‑150 (MQ=255)

                   aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                              <  2:1624432/150‑1 (MQ=255)

                     cccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTa                                                                                                                            >  2:2356134/1‑150 (MQ=255)

                      ccGCTTATTCAGATTAAGAATACGCTTTCATCAAAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTAt                                                                                                                           <  1:2740963/150‑1 (MQ=255)

                        gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGc                                                                                                                         <  1:444808/150‑1 (MQ=255)

                         cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                                                                                                        <  2:1840900/150‑1 (MQ=255)

                          ttATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATGGTc                                                                                                                       >  2:2993758/1‑150 (MQ=255)

                             ttCAGATTAAGAATCCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaat                                                                                                                    <  2:572464/150‑2 (MQ=255)

                                  aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATGCTCAATCAtga                                                                                                               >  2:66083/1‑148 (MQ=255)

                                   ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaa                                                                                                              <  2:3508236/147‑1 (MQ=255)

                                    taagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAAAAGTTTGATAATACAAGTAGACAAAAATTCGTAAAAATTAAATGAGAAATACTTCACAATATCGTGCTAAACTATGCTCAATCATGaaa                                                                                                             >  1:3741614/3‑150 (MQ=255)

                                       gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTGTGATATAACATGTAGACAAAAATTCCTAAAAATTTATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa                                                                                                          <  2:1969932/150‑1 (MQ=255)

                                        aaTACGCTTTCGACATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTCTGATATAACATGTAGACAAAATTTCGTAAAAATTAATTGTGAAATACTTCACTATCTCGTGCTATACTATGCTCAATCATgaaagaaa                                                                                                         <  2:154584/150‑1 (MQ=255)

                                         aTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                                                                        >  1:3089199/1‑150 (MQ=255)

                                          tACGCTTTCATCATAAATCATGATAGCGTTTTCGACAGCT‑A‑TGT‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAAGTCCTTGTGAAATACTTCACAATATCGTGCTGTACTATGCGCAATCATGAAAGAAAGc                                                                                                       <  2:2423611/150‑1 (MQ=255)

                                           aCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGGGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCa                                                                                                      >  1:3765559/1‑150 (MQ=255)

                                             gCTTTCATCATCAATCATGAGAGCGTTTGCGTCAACT‑A‑T‑T‑TTTATAATTAAAGAGTTTGATATAACATGTAGACAAAAATTCCTAAAAATTAATTGTGAAATACTTCACATTATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAgg                                                                                                    <  2:3870777/150‑1 (MQ=255)

                                             gCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAgg                                                                                                    >  1:641886/1‑150 (MQ=255)

                                               tttCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                                                                                                     <  2:2262398/147‑1 (MQ=255)

                                                ttCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaa                                                                                                 <  2:516084/150‑1 (MQ=255)

                                                  catcatAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaag                                                                                               >  1:3125584/1‑149 (MQ=255)

                                                  catcatAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTACACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaag                                                                                               >  2:2853934/1‑149 (MQ=255)

                                                   atcatAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                                                                                              >  2:2062163/1‑148 (MQ=255)

                                                    tcatAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGAc                                                                                             >  2:2547507/1‑150 (MQ=255)

                                                      atAAATCATGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACaa                                                                                           >  2:3075010/1‑150 (MQ=255)

                                                        aaaTCATGCTAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACCTGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                                                                         <  2:3263117/150‑1 (MQ=255)

                                                         aaTCATGATAGGGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATgg                                                                                        <  2:1780777/150‑1 (MQ=255)

                                                            caTGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaaa                                                                                     >  2:407649/2‑150 (MQ=255)

                                                             aTGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAAc                                                                                    >  2:2482376/1‑150 (MQ=255)

                                                             aTGATAGCGTTTTCGTCAACT‑A‑T‑TCTTC‑TAGTTACATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATGAAGTGTGAAATACTTCACAAGATCGTGCTATACTATGCTCAATCATGACTGAAAGCAGGGAAAGTCACTGGAAAc                                                                                    >  2:2679936/1‑150 (MQ=255)

                                                              tGATAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                                   >  1:410235/1‑150 (MQ=255)

                                                                aTAGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                                 >  1:3453800/1‑150 (MQ=255)

                                                                  aGCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGGAAAGACAATGGAAACTCAAg                                                                               >  2:1495793/1‑150 (MQ=255)

                                                                   gCGTTTTCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGc                                                                              >  2:1319909/1‑150 (MQ=255)

                                                                      ttttCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt                                                                           <  2:641886/150‑1 (MQ=255)

                                                                       tttCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGGAAAGACAATGGAAACTCAAGCAtta                                                                          >  2:821125/1‑149 (MQ=255)

                                                                         tCGTCAACTAT‑T‑T‑TTT‑TAGTTATATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAGAGACAATGGAAACTCAAGCATTACa                                                                        >  1:3251811/1‑150 (MQ=255)

                                                                         tCGTCAACTAT‑T‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                                        >  2:1276183/1‑150 (MQ=255)

                                                                                 ctatt‑t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                 <  1:3372516/146‑1 (MQ=255)

                                                                                  tatt‑t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGGAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTg                                                                >  2:1112102/4‑150 (MQ=255)

                                                                                   ttt‑t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGGTGa                                                               >  2:948906/3‑150 (MQ=255)

                                                                                    tt‑t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa                                                              <  1:984509/149‑1 (MQ=255)

                                                                                       t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACa                                                            <  2:1702526/150‑1 (MQ=255)

                                                                                       t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACa                                                            >  1:228817/1‑150 (MQ=255)

                                                                                           t‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGTACAGTa                                                         >  2:482305/1‑150 (MQ=255)

                                                                                           t‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                                         <  2:2405208/150‑1 (MQ=255)

                                                                                              aGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTatg                                                       >  2:44980/1‑148 (MQ=255)

                                                                                               gTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGc                                                      >  2:2934239/1‑150 (MQ=255)

                                                                                               gTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGc                                                      >  1:560221/1‑150 (MQ=255)

                                                                                                 tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCtt                                                    <  2:283060/150‑1 (MQ=255)

                                                                                                 tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCtt                                                    >  2:1870682/1‑150 (MQ=255)

                                                                                                  aaaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCttt                                                   >  2:678772/1‑150 (MQ=255)

                                                                                                   aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTg                                                  <  2:765229/150‑1 (MQ=255)

                                                                                                    aTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGa                                                 >  2:3728674/1‑150 (MQ=255)

                                                                                                     tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                                <  1:1903502/150‑1 (MQ=255)

                                                                                                         ttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATGCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                            <  2:2955869/150‑1 (MQ=255)

                                                                                                         ttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                            >  2:2194145/1‑150 (MQ=255)

                                                                                                           gATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                                          <  1:1970296/150‑2 (MQ=255)

                                                                                                           gATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                                          >  1:1797999/1‑149 (MQ=255)

                                                                                                           gATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                                          >  1:3541203/1‑149 (MQ=255)

                                                                                                               taacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGATGGACCGTATGCTTTGAAaaaacaaaac                                      >  1:3372291/8‑150 (MQ=255)

                                                                                                                  catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                                   >  1:3305263/5‑150 (MQ=255)

                                                                                                                   atgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACatat                                  >  1:777067/4‑150 (MQ=255)

                                                                                                                   atgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACatat                                  <  2:856031/147‑1 (MQ=255)

                                                                                                                   atgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACatat                                  >  1:1002719/4‑150 (MQ=255)

                                                                                                                      tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcg                               >  1:3037254/1‑148 (MQ=255)

                                                                                                                        gACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgct                             <  1:2698036/150‑5 (MQ=255)

                                                                                                                         aCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgctt                            <  1:1130590/150‑6 (MQ=255)

                                                                                                                         aCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgctt                            >  2:3199840/1‑145 (MQ=255)

                                                                                                                         aCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgctt                            >  2:2386832/1‑145 (MQ=255)

                                                                                                                          cAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTc                           <  2:3105554/150‑1 (MQ=255)

                                                                                                                           aaaaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTca                          >  2:3960791/1‑149 (MQ=255)

                                                                                                                             aaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAg                        >  2:3305464/1‑150 (MQ=255)

                                                                                                                              aaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGGTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGc                       >  1:701812/1‑150 (MQ=255)

                                                                                                                                 tCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGGTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaa                    >  1:96430/1‑150 (MQ=255)

                                                                                                                                  cGTGAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAGAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa                   <  1:2534132/150‑1 (MQ=255)

                                                                                                                                   gTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAt                  <  1:1767182/150‑1 (MQ=255)

                                                                                                                                    tAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATc                 >  2:1360162/1‑150 (MQ=255)

                                                                                                                                     aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc                <  1:870693/150‑1 (MQ=255)

                                                                                                                                      aaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCg               >  2:311358/1‑150 (MQ=255)

                                                                                                                                        aattaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt             >  1:2653855/7‑150 (MQ=255)

                                                                                                                                         attaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGACAGAAAGCAGGAAAAGACAATGGAAGCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTa            >  1:3824650/6‑150 (MQ=255)

                                                                                                                                         attaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTa            >  2:245279/6‑150 (MQ=255)

                                                                                                                                         attaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTa            >  2:1058169/6‑150 (MQ=255)

                                                                                                                                          ttaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAt           <  2:3398608/146‑1 (MQ=255)

                                                                                                                                           taattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAtg          <  2:3590929/147‑1 (MQ=255)

                                                                                                                                             attGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCACAATCCGTTAtgtg        >  1:3766134/2‑150 (MQ=255)

                                                                                                                                             attGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAtgtg        >  2:1588551/2‑150 (MQ=255)

                                                                                                                                              ttGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGc       >  1:2124076/1‑150 (MQ=255)

                                                                                                                                                gtgAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt     >  2:3159782/1‑149 (MQ=255)

                                                                                                                                                   aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGTAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCCTTATGTGCttcgg  >  1:3896830/1‑146 (MQ=255)

                                                                                                                                                   aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcgg  >  1:830904/1‑146 (MQ=255)

GCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑T‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGA  >  CP006881/3722985‑3723276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A