Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 113 306.8 9564096 98.5% 9420634 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,025 A→G L48L (CTT→CTC)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0250AG99.3% 1646.5 / NA 458L48L (CTT→CTC) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (199/253);  total (202/256)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 461 total aligned reads displayed.

GACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACT‑CATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGA  >  CP006881/3724878‑3725171
                                                                                                                                                    |                                                                                                                                                  
gcccGCCATGTAAAGATGCGGGCCCGCTCCCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAg                                                                                                                                                  <  1:1576290/148‑1 (MQ=255)
gACCGCCATGTAAAGGTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGATGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTACGCTTTCTGCTTTTCTTCCTCATTCACCGCCATCCGCCATTCCATAAAA‑GAAACAAg                                                                                                                                                  >  1:949554/1‑150 (MQ=255)
  ccGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTc                                                                                                                                                <  1:436170/150‑1 (MQ=255)
  ccGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCCTTGGCAATTCCTTGAAGGCTTGCAACGATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCTCAGCCATCCCCCATT‑CATAAAACGAAACGAGTc                                                                                                                                                >  2:1759782/1‑150 (MQ=255)
     ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGGcctg                                                                                                                                             >  2:3779613/1‑148 (MQ=255)
       aTGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCtgcg                                                                                                                                           <  1:4515905/150‑1 (MQ=255)
        tGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGGCCTGCgg                                                                                                                                          >  1:1848126/1‑150 (MQ=255)
         gTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGc                                                                                                                                         >  1:895242/1‑150 (MQ=255)
         gTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGc                                                                                                                                         >  2:4708114/1‑150 (MQ=255)
          tAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGcc                                                                                                                                        >  1:405940/1‑150 (MQ=255)
           aaaGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCt                                                                                                                                       >  2:4470850/1‑150 (MQ=255)
           aaaGATCCGGGCCCGCTCTCTCCTGCTTTGGCGTTTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCt                                                                                                                                       <  2:1670619/150‑1 (MQ=255)
             aGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGc                                                                                                                                     >  2:4541737/1‑150 (MQ=255)
                tGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAATGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCAca                                                                                                                                  >  2:3207098/1‑149 (MQ=255)
                tGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCAca                                                                                                                                  <  2:4256618/150‑2 (MQ=255)
                 gCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGGCCTGCGGCCTGCAcag                                                                                                                                 >  1:1900218/1‑148 (MQ=255)
                 gCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACACCTTCTTTTATAAAGCGCGGCGGCTCTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAACCCCCATT‑CATAAAACGAAACGAGTACTGCGGCCTGCAcag                                                                                                                                 >  2:2207569/1‑148 (MQ=255)
                  cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCCTCCGCCATT‑CATAAAACGAAACAAGTCCTGCGGCCTGCAcagc                                                                                                                                >  1:322312/1‑150 (MQ=255)
                   gggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCAcagcc                                                                                                                               >  2:540973/1‑150 (MQ=255)
                   gggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACACCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTCCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCAcagcc                                                                                                                               <  1:1698331/150‑1 (MQ=255)
                        cgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATC‑CATAAAACGAAACGGGTCCTGCGGCCCGCCCAGCCGCctt                                                                                                                          >  1:1676602/2‑150 (MQ=255)
                        cgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCACTCACAGCCATCCGCCATC‑CATAAAACGAAACGAGTCCTGCGGCCTGCACGGCCGCctt                                                                                                                          >  2:1334728/2‑150 (MQ=255)
                          ctctctCCTGCTTTGGTGATTCCTTGAAGGCTTGCAACCATCTTCTTTTATAAAGCGCGGCGGCCTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTc                                                                                                                        <  2:1858619/150‑1 (MQ=255)
                            ccctcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                                      <  1:3765561/148‑1 (MQ=255)
                             tctcCTGCTTTGGCGGTTCCTTGATGGCTTGCAAACATCTTCTTTTCTAAAGCGCGGCGGCCTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCctttcttt                                                                                                                     <  2:4675838/150‑1 (MQ=255)
                              caccTGCTTTGGCGATTCCTTGAAGGGTTGCAAACATTTTCTTTTATAAAGCGCCGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTg                                                                                                                    <  1:2023164/148‑1 (MQ=255)
                               tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTgg                                                                                                                   <  2:282045/150‑1 (MQ=255)
                                ccTGCTTTGGCGATTCCTTGAAGGCTTGCAACCATCTTCTTTTATAAAGCGCGGCGGCTTCCGCTTTCTGCTTTTCTTCCCCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGt                                                                                                                  <  2:1715505/150‑1 (MQ=255)
                                ccTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGt                                                                                                                  <  1:4501735/150‑1 (MQ=255)
                                ccTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGt                                                                                                                  >  2:647569/1‑150 (MQ=255)
                                  tGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                >  1:1109421/1‑150 (MQ=255)
                                   gCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATg                                                                                                               <  1:2556746/150‑1 (MQ=255)
                                   gCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATg                                                                                                               <  2:3543070/150‑1 (MQ=255)
                                   gCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCCTTCTTTGGTATg                                                                                                               >  2:4327777/1‑150 (MQ=255)
                                    cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGt                                                                                                              <  1:2992080/150‑1 (MQ=255)
                                        ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGc                                                                                                          <  1:2928699/150‑1 (MQ=255)
                                        ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGc                                                                                                          <  1:350336/150‑1 (MQ=255)
                                        ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGc                                                                                                          >  2:139026/1‑150 (MQ=255)
                                         gCGATTCCTTGAAGGCTTGCACACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACCAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                         <  1:67341/150‑1 (MQ=255)
                                          cGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTCTTCTTCCTGATTGACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCTCAGCCGCCCTTCTTTGGGATGGTCGCTg                                                                                                        >  1:4757283/1‑150 (MQ=255)
                                           gATCCCTTGAAGTCTTGCAAACATCTTCTTTTATAAAGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa                                                                                                       <  1:2330656/150‑1 (MQ=255)
                                              tCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCCCAGCCATCCGCCATT‑CCTAAAACCAAACGAGGCCTGCGGCCCGCACAGCCCCCTTTCTTTGGTTTGTACGCTGGGcc                                                                                                    >  2:2465633/1‑150 (MQ=255)
                                              tCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACCGCCGCCTTTCTTTGGTATGTACGCTGAGcc                                                                                                    >  2:3343999/1‑150 (MQ=255)
                                                 ttGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                 >  2:2314808/1‑150 (MQ=255)
                                                  tGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCCTTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGGATGGTCGCTGGGCCGGAt                                                                                                >  2:3034427/1‑150 (MQ=255)
                                                   gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGGGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGGGCCGGATa                                                                                               >  2:705203/1‑150 (MQ=255)
                                                   gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTCCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATa                                                                                               <  1:3312022/150‑1 (MQ=255)
                                                    aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAg                                                                                              <  1:640750/150‑1 (MQ=255)
                                                      ggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGAc                                                                                            >  2:3653630/1‑150 (MQ=255)
                                                      ggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGAc                                                                                            <  1:103771/150‑1 (MQ=255)
                                                          tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATT‑CATAAAACGGAACGAGTCCCGCGGCCTCCGCAGCCCCCCTTCCTTGGTATGTCCGCTGGGCCCACCAGACcgcg                                                                                        >  2:832026/1‑150 (MQ=255)
                                                           gCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCgg                                                                                       <  1:4679492/150‑1 (MQ=255)
                                                            cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGc                                                                                      <  1:1639644/150‑1 (MQ=255)
                                                                      cttTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGtt                                                                            <  1:2655799/150‑1 (MQ=255)
                                                                      cttTTATAAAGCGCGGCGGCTTCCGCTTTCTGCTTTTCTTCCTCATTCCCAGCCATCCGCCATT‑CATAAAACGAAACGAGCCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGtt                                                                            <  1:4141575/150‑1 (MQ=255)
                                                                          tataAAGCGCGGCGGTTTTCGCTTCCTGCTTTTCTTCCTCCTTCACAGCCATCCGCCATT‑CATAAAACGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTc                                                                        <  2:1951719/150‑1 (MQ=255)
                                                                           ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCCTTCTTTGGTATGGACGCTGGGCCGAATAGACCGCGGCAGCCGCAGGTTTTCt                                                                       >  1:2384065/1‑150 (MQ=255)
                                                                            taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTg                                                                      <  2:1048794/150‑1 (MQ=255)
                                                                              aaGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGGACGCTGAGCCGAATAGACCGCGGCAGGCGCAGTCTTTCTGTc                                                                    >  2:3265932/1‑150 (MQ=255)
                                                                              aaGCGCGGCGGCTTTCCCTTTCTGCTTTTCTTCCTCATTCCCAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACATGCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                    <  1:2086184/150‑1 (MQ=255)
                                                                                gcgcGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGACCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCg                                                                  <  1:14283/150‑1 (MQ=255)
                                                                                   cggcggCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTGCGCTGAGCCGAATAGACCCCGGCAGCCGCAGCTTGTCTGTCCgggg                                                               >  2:3110689/1‑148 (MQ=255)
                                                                                   cggcggCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                               <  1:3615265/150‑1 (MQ=255)
                                                                                      cggcTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCGATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCa                                                            <  1:422969/150‑1 (MQ=255)
                                                                                        gctttcgctttcTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGTCCGGCggcccc                                                          >  2:3870074/1‑145 (MQ=255)
                                                                                        gctttcgctttcTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCCCGGGTTTTCTGTCCGGCGTCAcc                                                          >  1:278609/1‑150 (MQ=255)
                                                                                         cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGGGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAccc                                                         >  2:115029/1‑150 (MQ=255)
                                                                                         cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGGACGCTGGGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAccc                                                         >  1:288672/1‑150 (MQ=255)
                                                                                              gCTTTCTGCTGTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGGGCCGAATAGGCCGCGGCAGCCCCAGGTTTTTTGTGCGAGGGCAcccccctc                                                    >  1:27398/1‑145 (MQ=255)
                                                                                               cTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGGGCCGAATAGACCGCGGCAGACGCGGTTTTTCTGGCCCGCGGCACCCGGGtca                                                   >  2:3159086/1‑150 (MQ=255)
                                                                                               cTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtca                                                   <  1:3113206/150‑1 (MQ=255)
                                                                                               cTTTCTGCTTTTCTCCCTCATCCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtca                                                   <  1:372659/150‑1 (MQ=255)
                                                                                                tttctGCTTTTCTTCCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtcat                                                  <  1:802511/150‑1 (MQ=255)
                                                                                                         ttcttcCTCATTCACAGCCATCCGCCATT‑CATAACACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                                         <  1:1009870/150‑1 (MQ=255)
                                                                                                         tcctcccTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGCCCTGCGGCCTGCACACCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                                         <  1:3875123/145‑1 (MQ=255)
                                                                                                            ttcCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAATGACCAt                                      >  1:1333892/1‑150 (MQ=255)
                                                                                                             tcCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCCGTTCATCCAAAAGACCATc                                     >  2:2106383/1‑150 (MQ=255)
                                                                                                             tcCTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGGCCGGCGTCACCCCGTTCATCAAAAAGACCacc                                     >  1:1177182/1‑148 (MQ=255)
                                                                                                               cTCATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGACGCCATTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGaccaacca                                   >  2:417717/1‑150 (MQ=255)
                                                                                                                 cATTCACAGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTTCGGCGGCACCCAGGTCCTCAAAAAGGCCATCcaca                                 >  2:2384921/1‑150 (MQ=255)
                                                                                                                      acaGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGGCGCAGGTTTTCTGTCCGGCGTCACCCAGGTCATCAAAAAGACCATCCACACCCgg                            >  2:3988722/1‑149 (MQ=255)
                                                                                                                        aGCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGGACGCTGGGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGTCCGGCGTCACCCCGTTCATCAAAAAGACCATCCACACCCGCaa                          >  1:3392495/1‑150 (MQ=255)
                                                                                                                         gCCATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGGGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCCGTTCATCCAAAAGACCATCCACACCCCTaaa                         >  1:1762332/1‑150 (MQ=255)
                                                                                                                          ccATCCGCCCTT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGCCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAg                        <  1:1456285/150‑1 (MQ=255)
                                                                                                                           cATCCGCCATT‑CATAACCCGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTCCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGa                       <  1:2445342/150‑1 (MQ=255)
                                                                                                                           cATCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACACCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGGCGCAGGTTTTCTGTCCGGCCGCACCCAGTTCTTCCAAAAGACCCTCCCCCCCCCTAAAGa                       >  1:3991613/1‑150 (MQ=255)
                                                                                                                            aTCCGCCATT‑CATAAAACGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAt                      <  1:3818453/150‑1 (MQ=255)
                                                                                                                            aTCCCCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAt                      <  1:544719/150‑1 (MQ=255)
                                                                                                                             tCCGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCCCACCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                     <  1:1268498/150‑1 (MQ=255)
                                                                                                                               cGCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACa                   >  1:1236604/1‑150 (MQ=255)
                                                                                                                                gCCATT‑CATAAAACGAAAGGAGTCCTGCGGCCAGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACaa                  <  1:507676/150‑1 (MQ=255)
                                                                                                                                gCCATT‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACaa                  <  2:4050111/150‑1 (MQ=255)
                                                                                                                                   att‑CATAAACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCa               <  1:150633/148‑1 (MQ=255)
                                                                                                                                   att‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCa               <  1:3153111/148‑1 (MQ=255)
                                                                                                                                     t‑CATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGGCCAACCACACCCGTAAAGATACAAGCaaa             >  1:734134/1‑150 (MQ=255)
                                                                                                                                       cATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAACCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCaaaa            >  2:223485/1‑149 (MQ=255)
                                                                                                                                           aaaCGACATGAGTCCTGCGGCCTGCACCGCCGCCTTTCTTTGGTGTGTACGCTGAGCCGTATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt        <  1:2189110/150‑1 (MQ=255)
                                                                                                                                            aacgaaacgaGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGGCACCCAGGTCCTCAAAAAGACCATCCACACCCGTAAAGATACaaacaaaccgttc       >  1:4632170/1‑145 (MQ=255)
                                                                                                                                              cGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCAt     <  2:1470380/150‑1 (MQ=255)
                                                                                                                                                aaaCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGTCGCAGTTTTTCTGTCCGGAGGCAAGCAGTTGAATAAGAAGACAGTCCACACCCGGAAAGGTACAAGCAAAGGATTCACAg   >  2:2066909/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACT‑CATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGA  >  CP006881/3724878‑3725171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A