Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F63 I1 R1 27 92.8 3572124 97.2% 3472104 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,186,578 A→G D410G (GAT→GGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,5780AG96.2% 383.8 / 2.7 104D410G (GAT→GGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (4/0);  new base G (45/55);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 4.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 105 total aligned reads displayed.

AGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCG  >  NC_000913/4186443‑4186714
                                                                                                                                       |                                                                                                                                        
aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAACCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATc                                                                                                                                      <  2:464386/140‑1 (MQ=255)
aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATc                                                                                                                                      <  2:462960/140‑1 (MQ=255)
  tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGTAGCTGTCGTTTGGGGTATcat                                                                                                                                    >  2:1064601/1‑138 (MQ=255)
  tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCt                                                                                                                                    <  2:1439682/140‑1 (MQ=255)
  tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCt                                                                                                                                    <  2:778775/140‑1 (MQ=255)
       tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACg                                                                                                                               >  1:882548/1‑140 (MQ=255)
       tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACg                                                                                                                               >  1:545818/1‑140 (MQ=255)
       tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACg                                                                                                                               >  1:1703703/1‑140 (MQ=255)
                    aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGa                                                                                                                  <  1:1114240/140‑1 (MQ=255)
                     tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                 >  1:1360643/1‑140 (MQ=255)
                       gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAaca                                                                                                               <  2:1430107/140‑1 (MQ=255)
                       gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAaca                                                                                                               <  2:1430215/140‑1 (MQ=255)
                        cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAacac                                                                                                              >  1:180664/1‑140 (MQ=255)
                         aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACAcc                                                                                                             <  1:88463/140‑1 (MQ=255)
                         aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACAcc                                                                                                             <  1:905535/140‑1 (MQ=255)
                            ggtgCTGTTCAAACCGTTCATCTAGGGCAAGCTGGAATTGCGTGGTATTGATACCACCTTTAATGCTGCGATGAAAATGGTTGTGCGCGATTAAGGTGTCGTTTGGGGTTTCATGGACTATGTTTTCGGCGAACACCCgg                                                                                                          <  2:1670322/137‑1 (MQ=255)
                            ggAGCTGTTCAAACCTTTCATCCTCGGCAAGCTGGAACTGCGTGGTCTGGCTTCCACCATTCAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTAACCGCGAACACCCgg                                                                                                          <  2:1581723/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:776673/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:1463552/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  2:321214/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  2:1713542/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:1759864/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  2:563313/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:1087820/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  2:1235205/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:151391/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:150815/140‑1 (MQ=255)
                            ggAGCTGTTCAAACCGTTCATATACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                          <  1:833186/140‑1 (MQ=255)
                              aGCTGTTCAAACCGTTCATCTACGGCAAGCTTGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                                                                        <  2:521481/140‑1 (MQ=255)
                              aGCTGTTCAAACCGTACATCTACGGCAAGCTGGAACTGCGTGGTCTAGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCTAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                                                                        <  2:877746/140‑1 (MQ=255)
                                   ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                   <  1:1096248/140‑1 (MQ=255)
                                   ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                   <  1:300183/140‑1 (MQ=255)
                                      aaaCAGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGaa                                                                                                <  1:382787/140‑1 (MQ=255)
                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                                                                               >  1:1500832/1‑140 (MQ=255)
                                        aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAcc                                                                                              >  1:1676943/1‑140 (MQ=255)
                                         ccGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCg                                                                                             <  1:1641897/140‑1 (MQ=255)
                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAATTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                         >  2:865597/1‑140 (MQ=255)
                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                         >  1:1234950/1‑140 (MQ=255)
                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                         >  2:477426/1‑140 (MQ=255)
                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                         >  1:1431258/1‑140 (MQ=255)
                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATACTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                         >  2:477409/1‑140 (MQ=255)
                                                  tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                                                    <  1:819358/140‑1 (MQ=255)
                                                  tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                                                    <  1:205098/140‑1 (MQ=255)
                                                   aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAct                                                                                   <  1:1054924/140‑1 (MQ=255)
                                                    cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGGTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActc                                                                                  >  2:806888/1‑140 (MQ=255)
                                                        aaGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                                              <  1:1064239/140‑1 (MQ=255)
                                                              gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                                        <  1:1554798/140‑1 (MQ=255)
                                                                aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                      <  2:442340/140‑1 (MQ=255)
                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGTCTCTGCACCGTCTGGGTAt                                                                  >  1:220651/1‑140 (MQ=255)
                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                                                                  >  1:220613/1‑140 (MQ=255)
                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                                                                  >  1:949530/1‑140 (MQ=255)
                                                                            tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGTAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                                                          >  2:854310/1‑140 (MQ=255)
                                                                            tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGAGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                                                          >  2:901635/1‑140 (MQ=255)
                                                                             gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                         <  2:1588318/140‑1 (MQ=255)
                                                                             gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                         <  2:914409/140‑1 (MQ=255)
                                                                             gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                         <  1:948731/140‑1 (MQ=255)
                                                                             gCTACCACCATTAAAGATGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGAAGAAGTTATCCGCGAACACCAGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                         <  1:857555/140‑1 (MQ=255)
                                                                              cTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                                        >  1:1152100/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTTAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  2:423035/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  1:851415/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  2:221853/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  1:566243/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  1:1671518/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  2:1433501/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  1:1533510/1‑140 (MQ=255)
                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                   >  2:1107083/1‑140 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTTGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTTCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               >  1:1332963/1‑140 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTTGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               >  1:1654028/1‑140 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               <  1:1534980/140‑1 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               <  2:550875/140‑1 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               >  2:1657176/1‑140 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               >  1:1332403/1‑140 (MQ=255)
                                                                                       ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCATGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                               <  1:261701/140‑1 (MQ=255)
                                                                                           aGCTGCGAAGAAAATGGTTGAGCGCGTAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTgt                                           >  2:601836/1‑139 (MQ=255)
                                                                                           aGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                                           >  1:899357/1‑140 (MQ=255)
                                                                                           aGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                                           >  1:1372425/1‑140 (MQ=255)
                                                                                            gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCT‑GGTATCCAGGCATTTGAACCGGTACTGATc                                         <  2:1722275/140‑1 (MQ=255)
                                                                                            gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAACGTCTGGGTATCCAGGCATATGAACCGGTACTGAt                                          <  2:1070595/140‑1 (MQ=255)
                                                                                             cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                                         <  2:1227340/140‑1 (MQ=255)
                                                                                             cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                                         <  1:636476/140‑1 (MQ=255)
                                                                                             cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTAGGTATCCAGGCATTTGAACCGGTACTGATc                                         <  2:1722702/140‑1 (MQ=255)
                                                                                               gCGACGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGGAATCCTGGACGAAGTTATCCGCGTACACCCGGTACGGCTGTACCGTTCACCGACGCGTCACCGTCTGGGTATCCAGGCCTTTGTACCGGTACTGATCGa                                       >  2:1008626/1‑140 (MQ=255)
                                                                                               gCGAAGAAAATGGTTGTGCGCGAAGAAGCTGTCGTTTGGCGTATCCTGGACGAAGTTATCCGCGAAAACCCGGTACTGCTGAACCGAGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                                       >  2:1071869/1‑140 (MQ=255)
                                                                                               gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                                       >  2:1125155/1‑140 (MQ=255)
                                                                                               gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                                       >  2:1604296/1‑140 (MQ=255)
                                                                                               gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGGCGGTTGGGGTAGCCTGGACGTAGTTATACGCGACAACCCGGTCCTGATGAACCGTGCACCGACTCTGCACCGTCTGGCTAGCCAGGCAGTTGACCCGGGCCTGAGCGa                                       >  2:1008625/1‑140 (MQ=255)
                                                                                                cGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGTAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                      >  2:156046/1‑140 (MQ=255)
                                                                                                cGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                      >  1:923560/1‑140 (MQ=255)
                                                                                                cGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACACTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                      >  1:923471/1‑140 (MQ=255)
                                                                                                  aagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAgg                                    <  2:392810/140‑1 (MQ=255)
                                                                                                               aGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAg                       >  2:1168042/1‑140 (MQ=255)
                                                                                                               aGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAg                       >  2:1167729/1‑140 (MQ=255)
                                                                                                                  gcgAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTg                    <  1:814426/140‑1 (MQ=255)
                                                                                                                  gcgAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTg                    <  1:905997/140‑1 (MQ=255)
                                                                                                                     aagaagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCAc                 >  1:1656343/1‑140 (MQ=255)
                                                                                                                        aagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCg              <  2:1646546/140‑1 (MQ=255)
                                                                                                                                 tttGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgt     <  2:1676943/140‑1 (MQ=255)
                                                                                                                                  ttGGGGTATCCTGGTCGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg    <  1:1425722/140‑1 (MQ=255)
                                                                                                                                  ttGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg    <  1:484236/140‑1 (MQ=255)
                                                                                                                                    ggggTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg  <  2:180664/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
AGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCG  >  NC_000913/4186443‑4186714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A