Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F63 I0 R1 109 725.1 24574764 99.0% 24329016 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,186,578 A→G 100% D410G (GAT→GGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,5780AG97.5% 3252.4 / 32.3 885D410G (GAT→GGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (12/10);  new base G (496/364);  total (509/376)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 892 total aligned reads displayed.

CCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACG  >  NC_000913/4186429‑4186724
                                                                                                                                                     |                                                                                                                                                  
      tCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCt                                                                                                                                              >  1:5078607/1‑150 (MQ=255)
         gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGa                                                                                                                                           <  1:10680741/150‑1 (MQ=255)
          cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGAc                                                                                                                                          >  2:9097969/1‑150 (MQ=255)
          cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGAc                                                                                                                                          >  2:11432693/1‑150 (MQ=255)
           aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACg                                                                                                                                         >  2:4586272/1‑150 (MQ=255)
             cAGTGCGGTCTGCCGAAGAAAATGGCACTGGATCTGTTCAAACCGTTCATATACGTCAAGCTGGAACTGCGTGGTCTTGCTACCACAATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGaa                                                                                                                                       <  2:3526059/150‑1 (MQ=255)
             cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGaa                                                                                                                                       <  2:4697402/150‑1 (MQ=255)
             cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGaa                                                                                                                                       <  2:2150087/150‑1 (MQ=255)
             cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGaa                                                                                                                                       <  2:2783062/150‑1 (MQ=255)
             cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                                                       <  2:2852964/150‑1 (MQ=255)
                tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGtt                                                                                                                                    >  1:7170832/1‑150 (MQ=255)
                tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGtt                                                                                                                                    >  2:5257447/1‑150 (MQ=255)
                  cGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGTGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTAt                                                                                                                                  >  2:6564663/1‑150 (MQ=255)
                    gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATcc                                                                                                                                >  2:7469834/1‑150 (MQ=255)
                      cTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCcgc                                                                                                                              <  1:1569993/150‑1 (MQ=255)
                       tGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCcgcg                                                                                                                             >  2:11183606/1‑150 (MQ=255)
                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                           >  2:10903592/1‑150 (MQ=255)
                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                           >  1:735631/1‑150 (MQ=255)
                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                           <  1:225200/150‑1 (MQ=255)
                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                           >  1:11442420/1‑150 (MQ=255)
                           gaagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAaca                                                                                                                         >  2:2268327/1‑150 (MQ=255)
                            aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAacac                                                                                                                        >  1:6713800/1‑150 (MQ=255)
                               aaaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCg                                                                                                                     >  1:1863969/1‑150 (MQ=255)
                               aaaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCg                                                                                                                     <  2:7635770/150‑1 (MQ=255)
                                aaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                                    <  1:2548418/150‑1 (MQ=255)
                                aaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                                    <  2:5500127/150‑1 (MQ=255)
                                aaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                                                    <  2:8831199/150‑1 (MQ=255)
                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGTAGTTATCCGCGAACACCCGGt                                                                                                                   >  2:156872/1‑150 (MQ=255)
                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGt                                                                                                                   >  1:7721008/1‑150 (MQ=255)
                                  aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                                                                                  <  2:9748262/150‑1 (MQ=255)
                                   tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTAc                                                                                                                 >  1:9989627/1‑150 (MQ=255)
                                   tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTAc                                                                                                                 >  1:9082857/1‑150 (MQ=255)
                                     gCACTGGAGCAGTTCAAACCGTTCATCTACGGAAAGCTGGAACTTCGTGGTCTTGCTACCACCATTAAAGCTGAGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGGTATCCTGGACGAACATAAACGCGAACACCCCGAActc                                                                                                               >  2:10643580/1‑149 (MQ=255)
                                      cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc                                                                                                              <  1:10894150/150‑1 (MQ=255)
                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                             >  1:2791678/1‑150 (MQ=255)
                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                             <  1:3182121/150‑1 (MQ=255)
                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGaa                                                                                                          <  1:10289182/150‑1 (MQ=255)
                                              cTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGt                                                                                                      <  1:10051782/150‑1 (MQ=255)
                                               tGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTg                                                                                                     <  2:5815939/150‑1 (MQ=255)
                                                    aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCATGCACCg                                                                                                >  1:11056047/1‑150 (MQ=255)
                                                      aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                                                              >  2:390197/1‑150 (MQ=255)
                                                      aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                                                              <  1:6387480/150‑1 (MQ=255)
                                                       ccGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGTGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAct                                                                                             <  2:4265314/150‑1 (MQ=255)
                                                         gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActct                                                                                           >  2:1910104/1‑150 (MQ=255)
                                                         gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActct                                                                                           >  1:11945417/1‑150 (MQ=255)
                                                          ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                                                                                          >  1:3895987/1‑150 (MQ=255)
                                                          ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                                                                                          >  2:4934569/1‑150 (MQ=255)
                                                           tCATCTACGGCCAGCTGGAACTGCGTGGTCGAGCTACCACCATTACAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGc                                                                                         <  2:7130362/150‑1 (MQ=255)
                                                               cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCg                                                                                     >  1:6066834/1‑150 (MQ=255)
                                                                 aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTc                                                                                   >  1:6531146/1‑150 (MQ=255)
                                                                 aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTc                                                                                   >  2:6683368/1‑150 (MQ=255)
                                                                  cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                                                  <  1:7527002/150‑1 (MQ=255)
                                                                  cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                                                  <  1:4583394/150‑1 (MQ=255)
                                                                  cGGCAAGCGGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                                                  <  2:9576060/150‑1 (MQ=255)
                                                                   ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                                                                                 <  1:8707056/150‑1 (MQ=255)
                                                                   ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATACTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                                                                                 >  1:2043753/1‑150 (MQ=255)
                                                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                <  2:4373677/150‑1 (MQ=255)
                                                                     cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGGTATCCTGGACGAAGTTATCCGCGCACACCCGGTACTGCAGAACCGTGCACCGACTCTGCACCGTCTggg                                                                               >  1:1056203/1‑150 (MQ=255)
                                                                      aaGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGt                                                                              >  1:9711103/1‑150 (MQ=255)
                                                                         cTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                                                           >  2:10201560/1‑150 (MQ=255)
                                                                            gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAg                                                                        <  1:11723122/150‑1 (MQ=255)
                                                                            gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCAGCACCGTCTGGGTATCCAg                                                                        >  2:11869285/1‑150 (MQ=255)
                                                                             aaCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg                                                                       <  1:4924187/150‑1 (MQ=255)
                                                                             aaCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg                                                                       <  2:9336537/150‑1 (MQ=255)
                                                                              aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGc                                                                      <  2:8409934/150‑1 (MQ=255)
                                                                                tGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                                                                    >  1:523688/1‑150 (MQ=255)
                                                                                   gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCCGTACTGCAGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCCGGCCTTTg                                                                 >  2:8245021/1‑150 (MQ=255)
                                                                                     ggTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGaa                                                               <  1:1303802/150‑1 (MQ=255)
                                                                                      gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                              <  1:6882144/150‑1 (MQ=255)
                                                                                         ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                                                           >  1:3491369/1‑150 (MQ=255)
                                                                                            cTACCACCATTAAAGGTGCGAAGATATTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                                                        <  2:7940699/150‑1 (MQ=255)
                                                                                             tACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACt                                                       <  2:6454867/150‑1 (MQ=255)
                                                                                               ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                                                     >  1:8139407/1‑150 (MQ=255)
                                                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                                                    <  1:9263010/150‑1 (MQ=255)
                                                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTATGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                                >  2:1341565/1‑150 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                               <  1:5409767/150‑1 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                               <  1:9015434/150‑1 (MQ=255)
                                                                                                          gCTGCGAAGAAATTGGTTGAGCGCGAAGAAGCTGTCTTTTGGGGTATCCTGGACGAAGTTATCCGCTAACACCCGTTACTGCTTAACCGTGCACCGACTCTGCACCGTCTGGGTATCCATGCATTTGAACCGGTACTGATCGAAGGTaaa                                          <  1:369528/150‑1 (MQ=255)
                                                                                                          gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTAACCTGGACGAAGTTATCCGCGAACAACCGGTAATGCTGAACCGTGCACCGACACAGCACCGTCTGGGTATACAGGCATTTGAACCCGTACTGATCGAAGGTaaa                                          >  1:11219903/1‑150 (MQ=255)
                                                                                                           cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATACTGGACGAAATTATCCGCGCACAACCCGTACTGCTGAACCCTGCACCGACAATTCACAGACAGGGTAAACAGGCAAATGAAACCGTACTGATCCAAgggtaat                                         >  1:4080751/1‑145 (MQ=255)
                                                                                                                  gaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa                                  >  2:10315631/1‑150 (MQ=255)
                                                                                                                  gaaAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa                                  >  2:5153620/1‑150 (MQ=255)
                                                                                                                     aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATACGCGAACACCCGGTACAGCTGAACCGTGCACCGCCTCTGCACCGGCTGGGTATCCAGGCAATTGTACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                               >  1:362861/1‑150 (MQ=255)
                                                                                                                     aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATACTTGACGAAGTTAACCGCGAACACCCGGTACAGCTGAACCGTGCACCGACGCATCACCGTCTGGGTATACAGGCATTTTAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                               >  1:8459147/1‑150 (MQ=255)
                                                                                                                        ggTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCa                            <  1:5757405/150‑1 (MQ=255)
                                                                                                                         gTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCAc                           >  2:2567314/1‑150 (MQ=255)
                                                                                                                         gTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCAc                           <  2:3258906/150‑1 (MQ=255)
                                                                                                                           tGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCAccc                         <  1:885508/150‑1 (MQ=255)
                                                                                                                               cgcgAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTg                     >  2:3688800/1‑150 (MQ=255)
                                                                                                                                gcgAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTgg                    >  2:3043974/1‑150 (MQ=255)
                                                                                                                                    agaagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAATTGAACCGGTACTGATCGAAGGGAAAGCTATCCAGCTGCACCCGCTGGTTtg                >  2:7294462/1‑150 (MQ=255)
                                                                                                                                     gaagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgt               >  2:860331/1‑150 (MQ=255)
                                                                                                                                      aagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg              <  2:6891304/150‑1 (MQ=255)
                                                                                                                                      aagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg              <  1:8380544/150‑1 (MQ=255)
                                                                                                                                       agCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGc             <  2:11675523/150‑1 (MQ=255)
                                                                                                                                        gcggTCGTTTGGGGGATCCGGGACGACGTTCTCCGCGCCCCCCCGGGCCGGCGGAACCGTGCCCCGCCTCGGCACCGTCTGGGTATCCCGGCATTTGCCCCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCCCCCGCTGGTTTGTGCg            <  2:415253/147‑1 (MQ=255)
                                                                                                                                        gCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg            <  2:1750926/150‑1 (MQ=255)
                                                                                                                                         cTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg           <  1:9387312/150‑1 (MQ=255)
                                                                                                                                          tGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGc          >  2:6149188/1‑150 (MQ=255)
                                                                                                                                                  ggggTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACg  <  1:4382919/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACG  >  NC_000913/4186429‑4186724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A