Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I0 R1 117 258.6 8574258 99.0% 8488515 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,186,578 A→G 63.7% D410G (GAT→GGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,5780AG63.7% 306.2 / 296.2 281D410G (GAT→GGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (52/50);  new base G (91/88);  total (143/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCC  >  NC_000913/4186429‑4186726
                                                                                                                                                     |                                                                                                                                                    
ccTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                                                                                      <  1:2551692/150‑1 (MQ=255)
  tGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGATCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGata                                                                                                                                                    >  2:2132560/1‑150 (MQ=255)
    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATc                                                                                                                                                  <  1:1417883/150‑1 (MQ=255)
    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATc                                                                                                                                                  <  1:3523357/150‑1 (MQ=255)
     gTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATcc                                                                                                                                                 <  2:821694/150‑1 (MQ=255)
       cTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTg                                                                                                                                               <  1:1841883/150‑1 (MQ=255)
       cTGAATCAGTGCGGTCTGCAGAAGAAAATGGCACTGGAGCTGTTAAAACATTTCATCTAAGGCAAGCTGGAACTGCTTGGACTTGCTAACACCATTAAAGCTGATAAGAAAATGGTTTAGCGCGAAGAAGCTGTCGTTTGTGatatacag                                                                                                                                               >  2:4260317/1‑146 (MQ=255)
        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                                                                                                                              >  2:3458492/1‑150 (MQ=255)
         gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGa                                                                                                                                             <  2:2284532/150‑1 (MQ=255)
           aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACg                                                                                                                                           >  1:3870842/1‑150 (MQ=255)
              aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAg                                                                                                                                        >  2:2280660/1‑150 (MQ=255)
              aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAg                                                                                                                                        <  2:1821298/150‑1 (MQ=255)
              aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCAAAGAAGCTGTCGTTTGGGATATCCTGGACGAAg                                                                                                                                        <  2:3621669/150‑1 (MQ=255)
                tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGtt                                                                                                                                      >  2:2292590/1‑150 (MQ=255)
                tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGtt                                                                                                                                      >  1:222194/1‑150 (MQ=255)
                    gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                                                                  >  2:2867222/1‑150 (MQ=255)
                    gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                                                                  >  1:302820/1‑150 (MQ=255)
                     tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcg                                                                                                                                 <  1:3887901/150‑1 (MQ=255)
                      cTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCcgc                                                                                                                                <  1:3630848/150‑1 (MQ=255)
                       tGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                                                                               >  2:3918835/1‑150 (MQ=255)
                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGaa                                                                                                                             >  1:4101898/1‑150 (MQ=255)
                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGt                                                                                                                     >  1:264575/1‑150 (MQ=255)
                                   tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTAc                                                                                                                   <  1:1628048/150‑1 (MQ=255)
                                    ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTAct                                                                                                                  <  2:3721090/150‑1 (MQ=255)
                                     gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActg                                                                                                                 <  2:1666528/150‑1 (MQ=255)
                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                               <  2:3166506/150‑1 (MQ=255)
                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                                               <  1:1171106/150‑1 (MQ=255)
                                         tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGa                                                                                                             <  1:1925775/150‑1 (MQ=255)
                                         tGGAGCTGTTCAAACCGGTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGGTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGa                                                                                                             >  2:296764/1‑150 (MQ=255)
                                         gggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGa                                                                                                             <  1:705258/149‑1 (MQ=255)
                                           gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                                                                                           >  2:4031587/1‑150 (MQ=255)
                                           gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                                                                                           <  2:2730233/150‑1 (MQ=255)
                                                 ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                                                     <  1:3925536/150‑1 (MQ=255)
                                                  tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                                                                                    <  1:199684/150‑1 (MQ=255)
                                                  tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                                                                                    <  1:1860331/150‑1 (MQ=255)
                                                    aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCg                                                                                                  >  1:1958325/1‑150 (MQ=255)
                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGTAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGa                                                                                                 >  1:518595/1‑150 (MQ=255)
                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGa                                                                                                 <  2:3253837/150‑1 (MQ=255)
                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGa                                                                                                 >  1:3776816/1‑150 (MQ=255)
                                                         gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActct                                                                                             >  2:731933/1‑150 (MQ=255)
                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAATAAATTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCGGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                                                          <  1:4083211/150‑1 (MQ=255)
                                                              tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCTAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAcc                                                                                        <  1:3601656/150‑1 (MQ=255)
                                                              tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAcc                                                                                        >  1:780252/1‑150 (MQ=255)
                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                                                                      >  1:152652/1‑150 (MQ=255)
                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                                                                      <  1:3118952/150‑1 (MQ=255)
                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                                                                      <  2:1388686/150‑1 (MQ=255)
                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGGACTTCTGAACCGTGCACCGACTCTGCAACGt                                                                                      >  1:1086927/1‑150 (MQ=255)
                                                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                  >  1:1769800/1‑150 (MQ=255)
                                                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                  <  1:2112054/150‑1 (MQ=255)
                                                                          tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                                                            <  1:4143702/150‑1 (MQ=255)
                                                                            gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAg                                                                          >  1:1244817/1‑150 (MQ=255)
                                                                              aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGc                                                                        <  1:3752572/150‑1 (MQ=255)
                                                                               cTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAGGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCa                                                                       <  2:1090791/150‑1 (MQ=255)
                                                                                 gCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                                     >  1:1570571/1‑150 (MQ=255)
                                                                                  cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                                                    <  2:1241559/150‑1 (MQ=255)
                                                                                      gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                <  1:585157/150‑1 (MQ=255)
                                                                                      gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                <  1:43480/150‑1 (MQ=255)
                                                                                        cTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                              <  2:2252219/150‑1 (MQ=255)
                                                                                         ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                                                             <  2:4160247/150‑1 (MQ=255)
                                                                                          tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGt                                                            <  1:566723/150‑1 (MQ=255)
                                                                                           gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                                           <  1:1023185/150‑1 (MQ=255)
                                                                                           gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                                                           <  2:2366444/150‑1 (MQ=255)
                                                                                             tACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACt                                                         <  2:538607/150‑1 (MQ=255)
                                                                                              accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAACGTCTGGGTATCCAGGCATTTGAACCGGTACTg                                                        >  2:1903680/1‑150 (MQ=255)
                                                                                                 accaTTACAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                                                     <  2:246195/150‑1 (MQ=255)
                                                                                                   caTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                                                   >  2:3804492/1‑150 (MQ=255)
                                                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                                  >  2:3334186/1‑150 (MQ=255)
                                                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                                  <  1:4037634/150‑1 (MQ=255)
                                                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa                                                  <  1:328984/150‑1 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                 <  1:912109/150‑1 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                 <  1:4221757/150‑1 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCCTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                 >  1:996184/1‑150 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                 <  1:830658/150‑1 (MQ=255)
                                                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                 <  1:3458492/150‑1 (MQ=255)
                                                                                                         aGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaa                                             <  1:3000011/150‑1 (MQ=255)
                                                                                                          gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa                                            <  1:2507627/150‑1 (MQ=255)
                                                                                                               gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGTGATATCCTGGACGAAGTTATCCGCGAACACCCCGTACAGCTGAACCGTGCACCGACACTGCACCGTCTGGGTATCCAGGCATTTTAAACGGTACTGATAGAAGGTAAAGCAAt                                       >  1:270476/1‑150 (MQ=255)
                                                                                                                  gaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa                                    <  2:2706779/150‑1 (MQ=255)
                                                                                                                  gaaAAAGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAATTGAACCGCTACTGATCGAAGGTAAAGCTATCCa                                    <  2:905178/150‑1 (MQ=255)
                                                                                                                   aaaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCAGGACGAAGTTATACGCGAACACCCCGTACTGCTGAACCGTGCACCCACACTGCACCGTCAGGGTATCCAGGCAATTGAACCCGTACAGATCGAAGGTAAAGCTATACAg                                   >  1:3697305/1‑150 (MQ=255)
                                                                                                                     aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                                 <  2:4182980/150‑1 (MQ=255)
                                                                                                                     aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATACAGCt                                 >  1:2378193/1‑150 (MQ=255)
                                                                                                                      aTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGGTGAAACGTGCACCGACTCTGCACCCTCTGGGTATACAGGCATTTGTACCGGTACAGATCGAAGGTAAATCTATACAGCTg                                >  1:3716894/1‑150 (MQ=255)
                                                                                                                             aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGc                         <  1:2151909/150‑1 (MQ=255)
                                                                                                                               cgcgAAGAAGCTGTCGTTTGGGATATAATGGACGAAGTTAAACGCGAACAACCCGTACAGCTGTACCGTGCACCGACTATGCACCGTCAGGGTATACAGGCATTTGAACCGGGACAGATAGAAGGTAAAGCAATACAGCAGCAcccccag                       >  1:2852159/1‑146 (MQ=255)
                                                                                                                                  gaagaaGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGtt                    <  1:1760172/150‑1 (MQ=255)
                                                                                                                                  gaagaaGCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGtt                    >  2:2827885/1‑150 (MQ=255)
                                                                                                                                    agaagCTGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtg                  >  2:4273419/1‑150 (MQ=255)
                                                                                                                                      aagCTGTCGTTTGGGGTATCGTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgtg                >  2:2823303/1‑150 (MQ=255)
                                                                                                                                       agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGc               <  2:302820/150‑1 (MQ=255)
                                                                                                                                         cTGTCGTTTGGGGTATCCGGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg             <  2:3778918/150‑1 (MQ=255)
                                                                                                                                         cTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg             <  2:2177997/150‑1 (MQ=255)
                                                                                                                                         cTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg             >  2:3342930/1‑150 (MQ=255)
                                                                                                                                         cTGTCGTTTGGGATATCCGGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg             <  1:3676748/150‑1 (MQ=255)
                                                                                                                                          tGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGc            >  2:1732832/1‑150 (MQ=255)
                                                                                                                                          tGTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGc            <  2:3757671/150‑1 (MQ=255)
                                                                                                                                           gTCGTTTGGGGTATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCa           <  2:866651/150‑1 (MQ=255)
                                                                                                                                              gTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCatat        >  1:2911964/1‑150 (MQ=255)
                                                                                                                                              gTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCatat        >  2:715576/1‑150 (MQ=255)
                                                                                                                                                  gggATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACg    <  1:1299577/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCC  >  NC_000913/4186429‑4186726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A