Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F66 I0 R2 122 57.2 2468826 97.5% 2407105 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,440,923 C→A 100% G36V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9230CA98.6% 226.5 / ‑4.6 68G36V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (31/36);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCT  >  NC_000913/3440792‑3441054
                                                                                                                                   |                                                                                                                                   
ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                             <  1:1207660/140‑1 (MQ=255)
ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                             <  1:1006721/140‑1 (MQ=255)
 cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCAc                                                                                                                            <  1:1130141/140‑1 (MQ=255)
   ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGc                                                                                                                          <  1:664124/140‑1 (MQ=255)
   ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCAATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGc                                                                                                                          <  1:1226354/140‑1 (MQ=255)
        tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaa                                                                                                                     <  1:1096640/140‑1 (MQ=255)
        tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaa                                                                                                                     <  1:80057/140‑1 (MQ=255)
        tCTTACTGATCGGCTTCTTTGGTGATGTACTCATGTAGTTCTACATCAATCTCAACCTCGGTCTCCGCTCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaa                                                                                                                     <  1:490575/140‑1 (MQ=255)
         cTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaaa                                                                                                                    <  1:957047/140‑1 (MQ=255)
              tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTc                                                                                                               <  1:269384/140‑1 (MQ=255)
                     cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                        <  1:433226/140‑1 (MQ=255)
                             gTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgc                                                                                                <  1:308541/140‑1 (MQ=255)
                              tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcg                                                                                               >  2:428473/1‑140 (MQ=255)
                                   tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcgg                                                                                          >  2:1118823/1‑139 (MQ=255)
                                   tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGCGTCACCTTGgcgtgcgt                                                                                          >  2:537680/1‑140 (MQ=255)
                                        aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                     >  2:130196/1‑140 (MQ=255)
                                        aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                     >  1:216016/1‑140 (MQ=255)
                                        aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                     >  2:313452/1‑140 (MQ=255)
                                        aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                     >  1:1185162/1‑140 (MQ=255)
                                             gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                >  1:491561/1‑140 (MQ=255)
                                                cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                             >  1:249702/1‑140 (MQ=255)
                                                 acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                            <  1:1057622/140‑1 (MQ=255)
                                                 acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                            <  1:1057224/140‑1 (MQ=255)
                                                     tCAATCTCAACCTCGGTCACCTCTCAACCCGGCATCGATGAGAGCAGAATACGGCGCATTGCGATACCCATAGTATGGACAAAGACACGCTCTAATGGCTCTAGGGGCACCTTGGCGTGCGTCGAACTCTCTTGCTCGat                                                                        >  2:1174838/1‑140 (MQ=255)
                                                     tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                        >  2:58349/1‑140 (MQ=255)
                                                       aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                      <  2:1048853/140‑1 (MQ=255)
                                                        aTCTAAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                             >  1:831820/1‑84 (MQ=255)
                                                        aTCTAAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                             <  2:831820/84‑1 (MQ=255)
                                                             aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                >  2:54165/1‑140 (MQ=255)
                                                                  cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                           >  2:507905/1‑140 (MQ=255)
                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCGACCAGGcgcg                                                          >  1:581309/1‑140 (MQ=255)
                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                          <  2:940295/140‑1 (MQ=255)
                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                          >  1:543567/1‑140 (MQ=255)
                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                          >  1:543554/1‑140 (MQ=255)
                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                          >  1:543378/1‑140 (MQ=255)
                                                                     tCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                        <  2:249186/140‑1 (MQ=255)
                                                                           cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCATGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                  >  1:261835/1‑140 (MQ=255)
                                                                           cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                  >  2:308577/1‑140 (MQ=255)
                                                                                       tCGATGAGAGCAGAATACGGCGCTGTGCGTTACCCAGAGTATTGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATTTCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                      <  2:167297/140‑1 (MQ=255)
                                                                                       tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                      <  1:445490/140‑1 (MQ=255)
                                                                                        cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAg                                     >  1:897795/1‑140 (MQ=255)
                                                                                        cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAg                                     >  1:899657/1‑140 (MQ=255)
                                                                                            gagaGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                 <  1:7291/140‑1 (MQ=255)
                                                                                            gagaGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                 <  1:983854/140‑1 (MQ=255)
                                                                                               agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                              >  1:710144/1‑140 (MQ=255)
                                                                                               agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                              >  1:142010/1‑140 (MQ=255)
                                                                                                    aaTACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                         >  1:730690/1‑140 (MQ=255)
                                                                                                    aaTACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                         >  1:487317/1‑140 (MQ=255)
                                                                                                    aaTACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                         >  1:352078/1‑140 (MQ=255)
                                                                                                    aaTACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                         >  1:148312/1‑140 (MQ=255)
                                                                                                        cGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                     <  1:1234330/140‑1 (MQ=255)
                                                                                                          gcgcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                   <  2:918276/140‑1 (MQ=255)
                                                                                                          gcgcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                   <  1:196225/140‑1 (MQ=255)
                                                                                                              aGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct               >  1:591682/1‑140 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  2:254449/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACACAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  2:636406/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  1:1038233/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  1:1110500/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  2:730690/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  1:1110599/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  1:465893/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  2:163983/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  1:83808/140‑1 (MQ=255)
                                                                                                               gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt              <  2:1074636/140‑1 (MQ=255)
                                                                                                                tGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt             >  2:414471/1‑140 (MQ=255)
                                                                                                                tGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt             >  2:234171/1‑140 (MQ=255)
                                                                                                                      aCCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt       <  1:1183473/140‑1 (MQ=255)
                                                                                                                           gagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt  <  1:868891/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
CCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCT  >  NC_000913/3440792‑3441054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A