Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F52 I0 R1 112 248.4 7913808 99.1% 7842583 149.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,634 C→T 100% E38K (GAG→AAG)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,6340CT100.0% 1080.2 / ‑6.7 286E38K (GAG→AAG) yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (140/145);  minor base G (1/0);  total (141/145)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

AACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAG  >  NC_000913/4633487‑4633780
                                                                                                                                                   |                                                                                                                                                  
aaCTGAAAGTGCTGTCACCATCGCTGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                  <  2:3871406/150‑1 (MQ=255)
aaCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCTAAGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                  <  2:651355/150‑1 (MQ=255)
aaCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                  <  1:2372506/150‑1 (MQ=255)
aaCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                  <  2:2945545/150‑1 (MQ=255)
  cTGACAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACg                                                                                                                                                <  2:2589256/150‑1 (MQ=255)
       aGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAAc                                                                                                                                           >  1:1054530/1‑150 (MQ=255)
          gCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGa                                                                                                                                        <  2:1757733/150‑1 (MQ=255)
            tGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATg                                                                                                                                      >  1:3463010/1‑150 (MQ=255)
                  cATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAatat                                                                                                                                >  1:1452838/1‑150 (MQ=255)
                  cATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAatat                                                                                                                                >  1:421478/1‑150 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            <  2:3749882/150‑1 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            <  1:2980281/150‑1 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            <  2:614855/150‑1 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            <  1:3293695/150‑1 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            <  1:3082228/150‑1 (MQ=255)
                       aTGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAg                                                                                                                           >  2:1197959/1‑150 (MQ=255)
                       aTGCCAGCTTCAATCACCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGCACACCGCTCTTGACGGCAACGGATGCAATATGGCAg                                                                                                                           >  2:1491276/1‑150 (MQ=255)
                          ccAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGAt                                                                                                                        >  1:2833950/1‑150 (MQ=255)
                            aGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATcc                                                                                                                      >  1:2619191/1‑150 (MQ=255)
                             gCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCt                                                                                                                     >  1:2449160/1‑150 (MQ=255)
                              cTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCtt                                                                                                                    >  2:2362724/1‑150 (MQ=255)
                                 cAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                                 <  2:2195483/150‑1 (MQ=255)
                                 cAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                                 <  2:2042107/150‑1 (MQ=255)
                                 cAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                                 <  2:1547712/150‑1 (MQ=255)
                                       ccACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGa                                                                                                           <  2:2140778/150‑1 (MQ=255)
                                       ccACCCAAAAATCAGCCTCTGGAAGAAAACGGCGGGCATTGGCTACACGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGa                                                                                                           >  1:2645811/1‑150 (MQ=255)
                                           ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGCTTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                       <  2:3636116/150‑1 (MQ=255)
                                           ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                       >  2:2513279/1‑150 (MQ=255)
                                           ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                       <  2:379983/150‑1 (MQ=255)
                                            cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                      >  1:2221589/1‑150 (MQ=255)
                                            cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                      >  2:3077951/1‑150 (MQ=255)
                                            cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                      >  1:1287378/1‑150 (MQ=255)
                                             aaaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                     >  1:1201627/1‑150 (MQ=255)
                                               aaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTATTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                   <  2:3369502/150‑1 (MQ=255)
                                                 aTCAGCCTCTGGAAGTATACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGaaa                                                                                                 <  2:3291868/150‑1 (MQ=255)
                                                    aGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGc                                                                                              <  2:88340/150‑1 (MQ=255)
                                                      ccTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTTGCCGAAGATCTCGTGAAATGCCt                                                                                            <  1:3426502/150‑1 (MQ=255)
                                                      ccTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCt                                                                                            <  2:2757155/150‑1 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  2:2430086/1‑150 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  1:935206/1‑150 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  2:544014/1‑150 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  1:2737168/1‑150 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  1:136365/1‑150 (MQ=255)
                                                         ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCa                                                                                         <  1:633234/150‑1 (MQ=255)
                                                           ggAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGa                                                                                       >  2:3010183/1‑150 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGCTTTCGTGCGCCAGCGCGCGGTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAAGATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      <  2:2623497/150‑1 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      <  2:1002984/150‑1 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      <  1:3003864/150‑1 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      >  1:1737125/1‑150 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      >  1:3695288/1‑150 (MQ=255)
                                                            gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGaa                                                                                      <  1:383094/150‑1 (MQ=255)
                                                             aaGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAAt                                                                                     <  1:1326961/150‑1 (MQ=255)
                                                              aGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATg                                                                                    <  1:2107853/150‑1 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  <  2:421478/150‑1 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  >  2:3676279/1‑150 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  >  1:2130472/1‑150 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  <  2:1452838/150‑1 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  >  1:294780/1‑150 (MQ=255)
                                                                 aaaCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACAACGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGcc                                                                                 >  2:2384551/1‑150 (MQ=255)
                                                                  aaCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCt                                                                                >  1:209922/1‑150 (MQ=255)
                                                                     ggcggGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGaa                                                                             <  2:459674/150‑1 (MQ=255)
                                                                            aTTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGc                                                                      >  1:1352461/1‑150 (MQ=255)
                                                                                gCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                  >  2:2668734/1‑150 (MQ=255)
                                                                                   aCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtg                                                               <  1:1038621/150‑1 (MQ=255)
                                                                                   aCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtg                                                               <  1:493714/150‑1 (MQ=255)
                                                                                   aCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtg                                                               <  1:531237/150‑1 (MQ=255)
                                                                                    cccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                              >  1:144519/1‑150 (MQ=255)
                                                                                     ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtggt                                                             <  2:1085372/150‑1 (MQ=255)
                                                                                      cGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtggtg                                                            <  1:233854/150‑1 (MQ=255)
                                                                                       gATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAACTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATttgtgg                                                           <  2:1326708/150‑1 (MQ=255)
                                                                                         tttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTc                                                         <  2:729649/150‑1 (MQ=255)
                                                                                             gTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcac                                                     <  1:1148955/150‑1 (MQ=255)
                                                                                                cgcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                  <  1:2037849/150‑1 (MQ=255)
                                                                                                  ccAGCGCGCGTTTCCACACTGCAAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCACTATGGCAGGATCCTTCGCCGAAGAACTCGTGAAATTCCTGCAGAATGGCCTGAATTTAAGCGGGATTGGTGGTCGCACAGACa                                                >  2:1935574/1‑150 (MQ=255)
                                                                                                    aGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAAc                                              >  2:3237231/1‑150 (MQ=255)
                                                                                                      cgcgcgTGTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                            <  1:229941/150‑1 (MQ=255)
                                                                                                       gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTg                                           >  1:3177197/1‑150 (MQ=255)
                                                                                                       gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTg                                           <  1:1482178/150‑1 (MQ=255)
                                                                                                       gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTg                                           <  2:3318820/150‑1 (MQ=255)
                                                                                                        cgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTgg                                          <  2:3548534/150‑1 (MQ=255)
                                                                                                         gcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGt                                         <  2:2125334/150‑1 (MQ=255)
                                                                                                         gcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGt                                         >  1:945656/1‑150 (MQ=255)
                                                                                                          cgTTTCCTCACGGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATAGGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTg                                        <  2:1501578/150‑1 (MQ=255)
                                                                                                                cTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                  <  1:3513704/150‑1 (MQ=255)
                                                                                                                 tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATc                                 >  2:2269325/1‑150 (MQ=255)
                                                                                                                 tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATc                                 >  2:847988/1‑150 (MQ=255)
                                                                                                                 tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATc                                 >  1:1942775/1‑150 (MQ=255)
                                                                                                                 tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATc                                 >  1:3541570/1‑150 (MQ=255)
                                                                                                                   aCTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAg                               <  1:3311434/150‑1 (MQ=255)
                                                                                                                    cTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGc                              <  1:741508/150‑1 (MQ=255)
                                                                                                                       ccAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAtt                           >  1:3797350/1‑150 (MQ=255)
                                                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTa                          >  1:3380321/1‑150 (MQ=255)
                                                                                                                            gggCTGTTCCGGTACTCCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCa                      <  2:2808712/150‑1 (MQ=255)
                                                                                                                              gCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGa                    <  2:136365/150‑1 (MQ=255)
                                                                                                                                 gTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGaaaa                 >  1:1014168/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTa              <  1:3724276/150‑1 (MQ=255)
                                                                                                                                             cGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag     >  1:3455585/1‑150 (MQ=255)
                                                                                                                                             cGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag     >  2:481718/1‑150 (MQ=255)
                                                                                                                                             cGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag     >  1:838676/1‑150 (MQ=255)
                                                                                                                                                tcttGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagcag  >  2:2847672/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAG  >  NC_000913/4633487‑4633780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A