Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F53 I19 R3	125	83.6	3029080	95.4%	2889742	127.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,082	A→C	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	147.9 / NA	39	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/22); total (17/22)

TAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTAGCA  >  CP006881/3724954‑3725213

taacccgcgGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCCCCCCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                                                           <  3:103901/134‑1 (MQ=255)

taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                                                           <  5:351930/139‑1 (MQ=255)

     cgcgGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCGTTGTTTGGTTTGTTCGGTGGGCCGGATAGGCCGCGGCCGGCGCAggt                                                                                                                      >  5:345956/1‑137 (MQ=255)

        ggcggcTTTCGCTTTCTGCTTTTCTTCCGCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                                                                                   <  2:15790/139‑1 (MQ=255)

                  gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                                                         <  7:141683/139‑1 (MQ=255)

                      tctgcttttCTTCCTCATTCACAGCCAACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCa                                                                                                      <  6:374533/133‑1 (MQ=255)

                              tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                                                              <  6:83426/138‑1 (MQ=255)

                              tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                                                              <  2:90607/138‑1 (MQ=255)

                                       ttCACAGCCAGCCGCCATTCATAAAAGGAAACTAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCGGCAGGTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                    <  5:207576/139‑1 (MQ=255)

                                              ccATCCGCCATTCATAAAACGACACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             <  2:98804/139‑1 (MQ=255)

                                              ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             <  7:269131/139‑1 (MQ=255)

                                              ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             <  3:155571/139‑1 (MQ=255)

                                              ccATCCCCCATTCATAAAACGACACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             <  2:275190/139‑1 (MQ=255)

                                                                   aaaCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCaa                                                        <  4:196003/139‑1 (MQ=255)

                                                                                    tGCACAGCCGCCTTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACCCCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa                                        >  3:344804/1‑138 (MQ=255)

                                                                                      tacagccgcGTTTGTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACAt                                     >  8:181449/5‑139 (MQ=255)

                                                                                      cacaGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACa                                      <  7:17518/138‑1 (MQ=255)

                                                                                              cctttCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGc                             <  5:140715/137‑1 (MQ=255)

                                                                                                     ttGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCtttt                      >  7:295166/1‑139 (MQ=255)

                                                                                                       ggTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCTTCCGCTTCTTTTTg                    >  8:366414/1‑139 (MQ=255)

                                                                                                         tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             >  4:106302/1‑80 (MQ=255)

                                                                                                         tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                             <  3:106302/80‑1 (MQ=255)

                                                                                                           tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATACCTCCGCTTGTTTTTgggcg                >  5:213435/1‑136 (MQ=255)

                                                                                                             tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc              >  3:222988/1‑139 (MQ=255)

                                                                                                             tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc              <  5:171506/139‑1 (MQ=255)

                                                                                                             tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc              <  6:287881/139‑1 (MQ=255)

                                                                                                             tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc              <  1:30091/139‑1 (MQ=255)

                                                                                                               cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca             <  5:378183/138‑1 (MQ=255)

                                                                                                                gCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGc           <  2:369958/139‑1 (MQ=255)

                                                                                                                  tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCACTCAGCCt         >  3:254182/1‑139 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                                                          <  3:262190/89‑1 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                                                          >  4:262190/1‑89 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtt        >  1:221622/1‑139 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtt        >  7:11222/1‑139 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtt        >  4:288826/1‑139 (MQ=255)

                                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCACTACAACAAAAAGACCATCCACACCCCTAAACATACAAGCAAACTTATCATATAAAAACATCCCTGCGATTCTGTATTgcagtcag            >  6:375818/1‑135 (MQ=255)

                                                                                                                         aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGCCtttttagc  >  4:46258/1‑135 (MQ=255)

                                                                                                                         aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGCCtttttag   >  6:256086/1‑135 (MQ=255)

                                                                                                                         aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCAGCCACACCCGTAAAGAAACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGGCAGCCtttttagc  >  1:118651/1‑135 (MQ=255)

TAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTAGCA  >  CP006881/3724954‑3725213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A