Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F55 I0 R1 217 81.2 3243344 95.0% 3081176 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,849 A→T 100% intergenic (‑23/+17) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,8490AT100.0% 207.3 / NA 61intergenic (‑23/+17)U712_19200/U712_19205SacPA operon antiterminator/Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/36);  total (25/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCT  >  CP006881/3724715‑3724974
                                                                                                                                      |                                                                                                                             
tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                           >  5:295524/1‑139 (MQ=255)
tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                           >  5:235169/1‑139 (MQ=255)
tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                           >  1:282820/1‑139 (MQ=255)
tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                           >  8:226063/1‑139 (MQ=255)
   gatCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGGtttt                                                                                                                         >  5:62571/3‑138 (MQ=255)
    atCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCCTCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGa                                                                                                                       >  2:29767/2‑139 (MQ=255)
      gaTTTTTCTTGTTTTGAAAAGCGATTCCCGGTCGCTTCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATATTTTTTCAATAGGTTATCTCTCGAGCCAATTTGCTTTTgtc                                                                                                                      <  7:61516/137‑3 (MQ=255)
        tttttCTTTTTTTGAAAAGCGATTCCCGGTCCCATCAATATTTTTTAATAATAATAATATTTTATTAAATAATAATTTTTTTTTAATACTTTATAGATTTTCAATAGGTTATATCTACAGCCAATTTTCTTTTGAATgg                                                                                                                   >  4:307675/1‑139 (MQ=255)
             cTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGAtt                                                                                                               <  1:215026/138‑1 (MQ=255)
                 ttttGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTAc                                                                                                           <  4:255366/138‑1 (MQ=255)
                 ttttGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTAc                                                                                                           <  8:138084/138‑1 (MQ=255)
                           cGATTCCCGGGCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAc                                                                                                <  6:337082/139‑1 (MQ=255)
                             aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              <  8:181705/139‑1 (MQ=255)
                             aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              <  6:295524/139‑1 (MQ=255)
                             aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              <  6:92469/139‑1 (MQ=255)
                             aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              <  2:282820/139‑1 (MQ=255)
                             aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTAACCg                                                                                              <  5:306035/139‑1 (MQ=255)
                                cccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                            <  7:226063/135‑1 (MQ=255)
                                cccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                            <  7:132140/135‑1 (MQ=255)
                                cccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                            <  4:139866/135‑1 (MQ=255)
                                cccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                            <  3:17953/135‑1 (MQ=255)
                                cccggTCACATCTCGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTGTCAAGACAATTTGCTTTTGACTGGTATTTTACCTCCTTTTGTCCGcc                                                                                            <  1:198245/135‑1 (MQ=255)
                                 ccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCa                                                                                           <  3:243810/136‑1 (MQ=255)
                                 ccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCAt                                                                                          <  1:242290/137‑1 (MQ=255)
                                     tCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTaa                                                                                      <  1:399832/139‑1 (MQ=255)
                                     tCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTaa                                                                                      <  6:235169/139‑1 (MQ=255)
                                     tCCCATCACGATATTTTCCTGCTCATCCTCTTTTATTAAAGCCGCATTGTTTTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTaa                                                                                      <  8:174039/139‑1 (MQ=255)
                                     gcccATCACGATTTTTTCCTGATTATCCTCTTTTTATAAAGCCGCTTTGTTGTTTAATACTTTATAGATTATCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTaa                                                                                      <  8:168587/138‑1 (MQ=255)
                                        cATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTAATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTa                                                                                       >  3:394598/1‑135 (MQ=255)
                                               aTTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAc                                                                                                <  5:128445/119‑1 (MQ=255)
                                               aTTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAc                                                                                                >  6:128445/1‑119 (MQ=255)
                                               aTTTTTTACTGATCATCCTATTTTAATAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                            <  6:339264/139‑1 (MQ=255)
                                                ttttttCCTGATCATACTATTTTATTAAATCCGAATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGGTTTTGACTTTGATTTTACCTCCTTTTGACAGCCATGTAAAGATGCGGGc                                                                            <  3:214982/138‑1 (MQ=255)
                                                     tcgtGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTTTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                       <  4:366283/135‑1 (MQ=255)
                                                     tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                       <  8:345547/138‑1 (MQ=255)
                                                          atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc                                                                  <  3:203853/138‑1 (MQ=255)
                                                          atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc                                                                  <  4:220596/138‑1 (MQ=255)
                                                                    tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCg                                                       >  3:129401/1‑139 (MQ=255)
                                                                                gCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCATTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAgg                                           <  5:185384/139‑1 (MQ=255)
                                                                                 cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc                                          >  1:349052/1‑139 (MQ=255)
                                                                                  aTTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                       >  4:48060/1‑109 (MQ=255)
                                                                                  aTTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                       <  3:48060/109‑1 (MQ=255)
                                                                                              tACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtctt                             <  6:398580/139‑1 (MQ=255)
                                                                                                cTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATATGCATATGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCATTGAAGGCTTGCAAACAtcttct                           >  5:184417/1‑139 (MQ=255)
                                                                                                 tttATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATcttctt                          >  3:123694/1‑139 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              <  8:259888/69‑1 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                              >  7:259888/1‑69 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGcccg                                                                         >  4:386558/1‑88 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGcccg                                                                         <  3:386558/90‑3 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTACTTGAAGGCTTGCAAACATCTTCttt                         >  1:343828/1‑138 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTCACCGCCATGTAAAGATGAGGGCCCGCTCTCTCCTCGTTTGGCGATTCCTTGATGGCTTGAAAACATCTTCttt                         >  3:145172/1‑138 (MQ=255)
                                                                                                   tataGATTTTCAAGAGGTTATCTATACCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTATGACCGCAATGTAAAGATGCGGTCCCGCTCTCTCCTGCTTTGTCGATTCCTTGAAGGCTTGGAAACATCTTCttt                         >  7:234085/1‑138 (MQ=255)
                                                                                                       gATTTTCAAGATGTTATCTCTCCCTCAAATTTGCTTTTGCCTGGGTGTTTACCTCATTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCGTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATaa                    <  6:261563/139‑1 (MQ=255)
                                                                                                          tttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                            >  8:182605/1‑80 (MQ=255)
                                                                                                          tttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                            <  7:182605/80‑1 (MQ=255)
                                                                                                          tttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACAGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgc                 >  5:53913/1‑139 (MQ=255)
                                                                                                           ttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgcg                >  3:332985/1‑139 (MQ=255)
                                                                                                           ttCAAGAGGTTATCTCTCCCGCCAATTTGCTGTTGACTGGGATTTTCCCTCCTTTTGACCGCCATGTAAAGATGCGGTCCCTCGCTCTCGTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATTAAgcg                >  5:261563/1‑139 (MQ=255)
                                                                                                                  ggttATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTCCAAACATCTTCTTTTATAAAGCGCggcggc         >  7:170615/4‑139 (MQ=255)
                                                                                                                      aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGATGGCTTGCCAAGATCTTCTTTTATAAAGCGCGGCGGCGTTc     >  5:12697/1‑139 (MQ=255)
                                                                                                                           tcCCGCCAATTTGCTGTTGACTGGGATTTCACCTCCTTTTGCCCGCCATGTAACGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGACGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCt  <  4:77158/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                             
TGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCT  >  CP006881/3724715‑3724974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A