Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F55 I0 R1
|
217 |
81.2 |
3243344 |
95.0% |
3081176 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
3,724,902 |
A→C |
100% |
A89A (GCT→GCG) |
U712_19205 ← |
Uncharacterized protein ywcI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,724,902 | 0 | A | C | 100.0%
| 117.8
/ NA
| 36 | A89A (GCT→GCG) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/20); total (16/20) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC > CP006881/3724768‑3725036
|
tcgtGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTTTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 4:366283/135‑1 (MQ=255)
tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 8:345547/138‑1 (MQ=255)
atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc < 3:203853/138‑1 (MQ=255)
atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc < 4:220596/138‑1 (MQ=255)
tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCg > 3:129401/1‑139 (MQ=255)
gCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCATTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAgg < 5:185384/139‑1 (MQ=255)
cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc > 1:349052/1‑139 (MQ=255)
aTTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct > 4:48060/1‑109 (MQ=255)
aTTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 3:48060/109‑1 (MQ=255)
tACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtctt < 6:398580/139‑1 (MQ=255)
cTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATATGCATATGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCATTGAAGGCTTGCAAACAtcttct > 5:184417/1‑139 (MQ=255)
tttATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATcttctt > 3:123694/1‑139 (MQ=255)
tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTACTTGAAGGCTTGCAAACATCTTCttt > 1:343828/1‑138 (MQ=255)
tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTCACCGCCATGTAAAGATGAGGGCCCGCTCTCTCCTCGTTTGGCGATTCCTTGATGGCTTGAAAACATCTTCttt > 3:145172/1‑138 (MQ=255)
tataGATTTTCAAGAGGTTATCTATACCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTATGACCGCAATGTAAAGATGCGGTCCCGCTCTCTCCTGCTTTGTCGATTCCTTGAAGGCTTGGAAACATCTTCttt > 7:234085/1‑138 (MQ=255)
gATTTTCAAGATGTTATCTCTCCCTCAAATTTGCTTTTGCCTGGGTGTTTACCTCATTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCGTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATaa < 6:261563/139‑1 (MQ=255)
tttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACAGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgc > 5:53913/1‑139 (MQ=255)
ttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgcg > 3:332985/1‑139 (MQ=255)
ttCAAGAGGTTATCTCTCCCGCCAATTTGCTGTTGACTGGGATTTTCCCTCCTTTTGACCGCCATGTAAAGATGCGGTCCCTCGCTCTCGTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATTAAgcg > 5:261563/1‑139 (MQ=255)
ggttATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTCCAAACATCTTCTTTTATAAAGCGCggcggc > 7:170615/4‑139 (MQ=255)
aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGATGGCTTGCCAAGATCTTCTTTTATAAAGCGCGGCGGCGTTc > 5:12697/1‑139 (MQ=255)
tcCCGCCAATTTGCTGTTGACTGGGATTTCACCTCCTTTTGCCCGCCATGTAACGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGACGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCt < 4:77158/137‑1 (MQ=255)
cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttcttc < 3:307675/138‑1 (MQ=255)
cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttcttc < 2:349052/138‑1 (MQ=255)
aCCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtc < 1:102202/67‑1 (MQ=255)
aCCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtc > 2:102202/1‑67 (MQ=255)
cGCCAGTTAAACAGGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGACGGCTTGAAAACATTTTCTTTTATAAAGCGCGGCGGCTTTCGATTTCTGCTTTTCTACCTCATTCACAGCCACCCGCCATACATaaacc < 6:12697/139‑3 (MQ=255)
ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTACTCATTAACAGCCATCCGCCATTAAAAAAACg > 4:163999/1‑138 (MQ=255)
tGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAAc < 4:118480/139‑1 (MQ=255)
gTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTGGAAGGCTTGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAAc < 4:394598/138‑1 (MQ=255)
gTAAAGATGCGGGACCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAAc < 4:129401/138‑1 (MQ=255)
agccGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTGCAAGGCTTGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct < 6:62571/136‑2 (MQ=255)
aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTCCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct < 8:170615/139‑2 (MQ=255)
tGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAAAATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCATCCGCCATTCATAAAACGAAACGAGTcct < 6:53913/138‑2 (MQ=255)
cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg > 7:20362/1‑139 (MQ=255)
ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc < 3:163999/139‑1 (MQ=255)
|
TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC > CP006881/3724768‑3725036
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
