Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F53 I5 R2
|
122 |
92.4 |
3471984 |
95.3% |
3308800 |
125.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
3,725,049 |
T→C |
K40K (AAA→AAG) |
U712_19205 ← |
Uncharacterized protein ywcI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,725,049 | 0 | T | C | 96.9%
| 110.4
/ NA
| 32 | K40K (AAA→AAG) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (6/25); minor base G (1/0); total (7/25) |
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAA‑CGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAA > CP006881/3724919‑3725175
|
gCGATTCCTTGAAGGGTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCCTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 7:416567/138‑1 (MQ=255)
gAAGGCTTGAAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGt < 3:256480/135‑1 (MQ=255)
aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 7:21900/139‑1 (MQ=255)
aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCCTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 8:43493/139‑1 (MQ=255)
cgCTTGAAACCTTCTTCTTTATTAACGGGGGGCGGCTTTGGATTTGTGCTTTGCTTCCTCATTCCCCCCCACCCCCATTTCAT‑AAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 1:101369/138‑1 (MQ=255)
gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 5:61767/136‑1 (MQ=255)
gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa < 2:69459/138‑1 (MQ=255)
cAAACAGCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGcc < 5:359578/136‑1 (MQ=255)
taACGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCTTCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACTGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAg < 5:435830/136‑1 (MQ=255)
cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑c < 2:48684/139‑1 (MQ=255)
cTTTCGCTTTCTGCTTTTCTTCCTCAATCACAGCCATCCGCCATTCATAAAC‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCt < 5:257692/136‑1 (MQ=255)
gcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCCCCGCCGCCGTTGGTTTGGATGTTCGCGGAGGCGGATAGACCCCGGCCGCCCCCGGTTTGTGGT‑CCGGCggccc > 5:435630/3‑132 (MQ=255)
ttcCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTGGTTTTTTCGCTGAGGCGCATATAACCCGGCAGCCCCAGTTTTTCTGT‑GCAGCGGCACCCAGtt > 6:248871/1‑135 (MQ=255)
aTTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaaa < 1:114203/138‑1 (MQ=255)
aTTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaa < 1:108639/137‑1 (MQ=255)
ttCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaa < 6:425690/136‑1 (MQ=255)
aGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGAcc < 5:270749/136‑1 (MQ=255)
aGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGAcc < 3:104669/136‑1 (MQ=255)
cactCCGCCATTCATAAAA‑CGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 8:373078/136‑1 (MQ=255)
cctCCCCCATTAATAAAACCAAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 1:352659/137‑1 (MQ=255)
tCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGa > 1:61959/1‑129 (MQ=255)
tCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGa < 2:61959/129‑1 (MQ=255)
ccGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTGTTTGGTTTGTACGCTGAGCCGCATAGACCGCGGCAGCCGCAGTTTTTCTGTGCCGGGGGC‑CCCAGGTGATCCAAAACACCAACCCCAcc > 7:149195/1‑139 (MQ=255)
cattCATAAAA‑CGAAACGAGTCCTGCGGCCTGGACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCTCCCAGTTCATCAAAAAGACCATCCTCACCCGTa < 8:146262/136‑1 (MQ=255)
cattCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACAccc < 4:345412/133‑1 (MQ=255)
cattCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa < 1:232107/136‑1 (MQ=255)
attCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCg < 4:113986/134‑1 (MQ=255)
tAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAg < 4:164361/136‑1 (MQ=255)
aaaa‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTTTGTACGCGGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCCGCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAg > 3:185941/1‑135 (MQ=255)
tCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGT‑GCGGCGTCACCCAGTTCATAAAAAAGACCCTCCCAACCCGGAAATATACAAGCAAACCttt > 7:85545/1‑138 (MQ=255)
gcgGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCATACGTTTCATAg < 8:154652/139‑1 (MQ=255)
cTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa < 3:164087/136‑1 (MQ=255)
acagccgccGTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa > 3:417143/4‑135 (MQ=255)
acagccgcGTTTGTTTGGTATGTTCGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑GCGGCGTGCCCCAGGTCATGAAAAAGACCCTCCACACCCCTAAAGAGACATTCATACGTTTTCTAGacaac > 4:410987/4‑134 (MQ=255)
|
GCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAA‑CGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAA > CP006881/3724919‑3725175
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
