Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F53 I0 R2 221 66.0 2680176 94.9% 2543487 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,392 A→C 100% L145L (CTT→CTG)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,3920AC100.0% 374.3 / ‑6.6 99L145L (CTT→CTG) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (55/43);  total (55/44)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCA  >  CP006881/3724261‑3724527
                                                                                                                                   |                                                                                                                                       
tttaaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGaca                                                                                                                                  <  7:141105/136‑1 (MQ=255)
ttAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGaca                                                                                                                                  <  5:231738/139‑1 (MQ=255)
   aaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACg                                                                                                                                <  3:199692/138‑1 (MQ=255)
   aaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTATCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCCACTTCATGCAGAGACACGc                                                                                                                               >  6:105754/1‑139 (MQ=255)
        ttggtttATTTTCTCTATCATTTCTTTGATCATGGTCGGATGCTGCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAAt                                                                                                                          <  8:156878/133‑1 (MQ=255)
          gTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATg                                                                                                                         <  8:91793/138‑1 (MQ=255)
          gTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  8:246312/139‑1 (MQ=255)
             ttATTTTCTCTATCATTTCTTTGATCATTGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGtctc                                                                                                                     >  8:43678/2‑139 (MQ=255)
              tATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTctct                                                                                                                    >  6:270706/1‑139 (MQ=255)
                     tctATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACAt                                                                                                             >  6:135849/1‑139 (MQ=255)
                     tctATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACAt                                                                                                             >  2:132614/1‑139 (MQ=255)
                        aTCATTTCTTTGATCATGGTCGTATTCTTCAGCGAAGAATACCTGCTCTCCGCCTCCATCTTCGCCGTATGGAAGTTAAGTGCAAGAAAAACATCTTCCTCTTAAGGCAGAGACACGCCCCATGTAGATTTTACaattc                                                                                                          >  6:207502/1‑135 (MQ=255)
                         tCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTc                                                                                                          <  5:270706/138‑1 (MQ=255)
                               cTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGc                                                                                                   >  2:30003/1‑139 (MQ=255)
                                         ggTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCca                                                                                                >  4:18295/1‑132 (MQ=255)
                                         ggTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCca                                                                                                <  3:18295/132‑1 (MQ=255)
                                         ggTCGTATGCTTCAGCGCTGAATAAATGCTCTCCGCAACCATCTTCTCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGATACACCCCCAATGTCTATTTTACATGTCAGCTCGACCACAgtac                                                                                          >  7:10096/1‑135 (MQ=255)
                                          gTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  1:289248/138‑1 (MQ=255)
                                          gTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  1:132614/138‑1 (MQ=255)
                                          gTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  7:17703/138‑1 (MQ=255)
                                              tATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGAtct                                                                                     >  1:127067/1‑138 (MQ=255)
                                                  cTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATa                                                                                 <  6:178285/138‑1 (MQ=255)
                                                   ttCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCatat                                                                                >  6:81612/1‑138 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCTCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               <  8:65263/137‑1 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  7:208141/1‑96 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  8:208141/96‑1 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGCTCTCATATTCt                                                                             >  2:255237/1‑139 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               <  6:220592/137‑1 (MQ=255)
                                                     cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                             >  4:98102/1‑139 (MQ=255)
                                                             aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTtata                                                                     >  2:122018/1‑139 (MQ=255)
                                                             aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTtata                                                                     >  4:3628/1‑139 (MQ=255)
                                                             aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCACAGACACGCCCAAAGTCTCTTTTACATGTCCGATCGCCCCCAGGCCGGTCTCATATTCTTTTTta                                                                       >  2:1503/1‑137 (MQ=255)
                                                              aTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATag                                                                    <  3:98102/139‑1 (MQ=255)
                                                                  aTGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGac                                                                                                                                   <  2:311526/72‑1 (MQ=255)
                                                                  aTGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGac                                                                                                                                   >  1:311526/1‑72 (MQ=255)
                                                                  aTGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAGTGTCTCTTTTACATGTCCGCTCg                                                                                                    >  4:272356/1‑103 (MQ=255)
                                                                  aTGCGCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCg                                                                                                    <  3:272356/103‑1 (MQ=255)
                                                                    gCTCTCCGCATCCATCTTCGCCGTATGGATGTGAATGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  1:122596/135‑1 (MQ=255)
                                                                    gCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  2:122596/1‑135 (MQ=255)
                                                                     ctctCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  2:75746/134‑1 (MQ=255)
                                                                     ctctCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  1:75746/1‑134 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGTTGTGAAGGGCAAAATAACCCGCTACATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCTCCCAGAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  7:321627/1‑138 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACTCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         >  1:247526/1‑109 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  2:247526/109‑1 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  3:113322/109‑1 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         >  4:113322/1‑109 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  2:81810/132‑1 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  1:81810/1‑132 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCcttt                                                           >  5:197491/1‑139 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCcttt                                                           >  5:25701/1‑139 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  8:300942/1‑138 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  2:311179/1‑138 (MQ=255)
                                                                       ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  3:157912/1‑138 (MQ=255)
                                                                                 atcTTCTCCGTTTGGCTGGTAAGGGCAATATCACCCTCTTCCTCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCGACATGCCTATCTCATATTCTTTTTTATAGATCGCCTTTATCTCATgca                                                 <  5:207502/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  7:156227/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  7:43678/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  1:30003/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  1:255237/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  5:169526/139‑1 (MQ=255)
                                                                                 atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  5:105754/139‑1 (MQ=255)
                                                                                  tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  8:200405/67‑1 (MQ=255)
                                                                                  tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  7:200405/1‑67 (MQ=255)
                                                                                  tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTAACTCATgcag                                                >  8:239263/1‑139 (MQ=255)
                                                                                    ttCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  8:45640/1‑119 (MQ=255)
                                                                                    ttCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  7:45640/119‑1 (MQ=255)
                                                                                         cGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTa                                         <  8:122275/139‑1 (MQ=255)
                                                                                           tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAtt                                       >  1:313370/1‑139 (MQ=255)
                                                                                           tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                        >  6:251899/1‑138 (MQ=255)
                                                                                                   tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACCCGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACaa                               >  6:214497/1‑139 (MQ=255)
                                                                                                   tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGcac                                 >  1:766/1‑137 (MQ=255)
                                                                                                     aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         >  8:5680/1‑79 (MQ=255)
                                                                                                     aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  7:5680/79‑1 (MQ=255)
                                                                                                          cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  7:17867/114‑1 (MQ=255)
                                                                                                          cAATATAACCAGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 >  8:17867/1‑114 (MQ=255)
                                                                                                             tataACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAtt                     >  4:29428/1‑139 (MQ=255)
                                                                                                                 aCCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGa                 <  2:313370/139‑1 (MQ=255)
                                                                                                                  cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  7:131867/89‑1 (MQ=255)
                                                                                                                  cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  8:131867/1‑89 (MQ=255)
                                                                                                                  cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGaa                >  8:62565/1‑139 (MQ=255)
                                                                                                                    cGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATc              >  7:114894/1‑139 (MQ=255)
                                                                                                                     gCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATcc             >  2:220239/1‑139 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGAt                                                                                                      <  6:108513/48‑1 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGAt                                                                                                      >  5:108513/1‑48 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCTCAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  8:283234/84‑1 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  5:304927/61‑1 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         >  6:304927/1‑61 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  3:78689/1‑84 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  7:283234/1‑84 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  4:78689/84‑1 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 >  8:222922/1‑101 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                 <  7:222922/101‑1 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt           >  3:282287/1‑139 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt           >  5:225141/1‑139 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt           >  4:145775/1‑139 (MQ=255)
                                                                                                                       ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt           >  1:238315/1‑139 (MQ=255)
                                                                                                                       ttCATATTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGAGCCTTTTTCTCATTCAGCAATTTATTCTGAACAAGCAGCCACTTTTGAATCCtt           >  7:328497/1‑139 (MQ=255)
                                                                                                                               cAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCg    >  4:311108/1‑138 (MQ=255)
                                                                                                                                aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  6:25701/139‑1 (MQ=255)
                                                                                                                                aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  7:62565/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCA  >  CP006881/3724261‑3724527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A