Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F58 I1 R3
|
118 |
63.0 |
2369054 |
95.4% |
2260077 |
127.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,700,146 |
C→T |
V167I (GTT→ATT) |
U712_13910 ← |
Lon protease 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,700,146 | 0 | C | T | 100.0%
| 197.5
/ NA
| 55 | V167I (GTT→ATT) | U712_13910 | Lon protease 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (32/23); total (32/23) |
TCTCAATCTCCAGCACTTCTTTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAACGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCGTCTTCTGTAT > CP006881/2700020‑2700280
|
tctcAATCTCCAGCACTTCTTTTTCATTATTAATAAAATCAATGACGTTGGTCAGCCTGTCATAGACATCAGCCGTGTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCa < 1:16114/138‑1 (MQ=255)
cAGGACTTCTTTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCAGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCACCgg > 4:144796/1‑138 (MQ=255)
cAGCACTTCTTTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCgg < 5:173008/138‑1 (MQ=255)
ttcttTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCg < 6:84088/139‑1 (MQ=255)
cttTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGat > 6:61193/1‑139 (MQ=255)
cttTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGa > 7:109881/1‑138 (MQ=255)
tCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCTATATCAg > 8:43761/1‑139 (MQ=255)
tCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAg > 8:38042/1‑139 (MQ=255)
ttaATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCg > 3:113899/1‑139 (MQ=255)
aaaTCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCg < 7:38042/138‑1 (MQ=255)
cAATGACCTTGTTCAGACTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGTCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGt > 5:104660/1‑139 (MQ=255)
cTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATTATGTCTGCCATTCTCCCCGGCTCATCGATATCAGAAACCGCAGCGTACGTTTCGGcc > 1:173258/1‑139 (MQ=255)
tGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTACCCGCAGCGTACGTTTCGGcc > 8:147626/1‑137 (MQ=255)
gTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGcc > 4:51736/1‑136 (MQ=255)
ttCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATTTCCGTAACCGCAGCGTACTTTTCGGcc > 7:147665/1‑135 (MQ=255)
ttCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCGCTTCGATATCAGTAACCGCAGCGTACGTTTCGGcc > 7:147662/1‑135 (MQ=255)
ccTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGAttttt > 1:244647/1‑139 (MQ=255)
ccTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGAttttt > 2:190204/1‑139 (MQ=255)
tCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGAtttttt > 5:269807/1‑136 (MQ=255)
tCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTa > 1:120064/1‑138 (MQ=255)
tCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTGCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGAtttttt > 8:103154/1‑136 (MQ=255)
tGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGaaa < 8:109881/138‑1 (MQ=255)
cAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGat < 6:144428/88‑1 (MQ=255)
cAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGat > 5:144428/1‑88 (MQ=255)
ccGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTa < 3:51736/139‑1 (MQ=255)
ccGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTa < 6:104660/139‑1 (MQ=255)
ccGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTa < 5:114405/139‑1 (MQ=255)
actatCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg < 2:93469/137‑1 (MQ=255)
aaTATCCTTCGTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg < 6:291196/139‑1 (MQ=255)
aaTATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg < 7:43761/139‑1 (MQ=255)
aaTATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg < 4:113899/139‑1 (MQ=255)
atatCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGt > 8:144570/1‑139 (MQ=255)
tCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGt < 2:120064/139‑1 (MQ=255)
tCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGt < 2:265631/139‑1 (MQ=255)
cTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAg > 1:154384/1‑138 (MQ=255)
aTCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCa < 4:295601/137‑1 (MQ=255)
aTCCTTTAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCa < 1:190204/137‑1 (MQ=255)
tAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGTCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCg > 4:289874/1‑138 (MQ=255)
tAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTa < 4:150464/111‑1 (MQ=255)
tAGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTa > 3:150464/1‑111 (MQ=255)
aGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGt < 1:44468/124‑1 (MQ=255)
aGTTTAAGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGt > 2:44468/1‑124 (MQ=255)
aaGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGaaa < 1:123424/95‑1 (MQ=255)
aaGGGGCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGaaa > 2:123424/1‑95 (MQ=255)
ggggCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGc > 2:86131/1‑139 (MQ=255)
ggggCAGATGAGAAGAAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCTCAGCTTAAGTTTCGGCCGAGATTTTTTTAGAAATTTTTATTTACTGATCAAAGTGGTCTAGCAAAGTCCTCATCAATGc > 4:92624/1‑139 (MQ=255)
gCAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTc < 6:269807/139‑1 (MQ=255)
cAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg > 7:35618/1‑109 (MQ=255)
cAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAg < 8:35618/109‑1 (MQ=255)
cAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCg > 7:29271/1‑139 (MQ=255)
cAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCg > 6:102589/1‑139 (MQ=255)
cAGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCg > 4:155608/1‑139 (MQ=255)
aGATGAGAAGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCGt > 8:100/1‑139 (MQ=255)
aaGCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGGTTCGGCCGAGATGTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCAGCAATGCTGCCTCGTCTGt > 1:75010/1‑139 (MQ=255)
gCAATGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCGTCTTCTGTAt < 2:154384/139‑1 (MQ=255)
|
TCTCAATCTCCAGCACTTCTTTTTCATTATTAATAAAATCAATGACCTTGTTCAGCCTGTCTTTGACATCAGCCGTTTCTAAAATATCCTGCTTATCCTTTAGTTTAAGGGGCAGATGAGAAGCAACGATGTCTGCCATTCTCCCCGGCTCTTCGATATCAGTAACCGCAGCGTACGTTTCGGCCGAGATTTTTTTAGAAATTTTTATGTACTGATCAAAGTGGTCTAGCAAAGTCCGCATCAATGCTTCGTCTTCTGTAT > CP006881/2700020‑2700280
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
