Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F58 I0 R3
|
223 |
82.2 |
2922920 |
95.2% |
2782619 |
126.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
3,553,118 |
T→G |
24.2% |
A471A (GCT→GCG) |
U712_18215 → |
Uncharacterized protein ywqB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,553,118 | 0 | T | G | 24.2%
| 55.8
/ 2.7
| 35 | A471A (GCT→GCG) | U712_18215 | Uncharacterized protein ywqB |
| Reads supporting (aligned to +/- strand): ref base T (2/23); new base G (7/2); total (10/25) |
| Fisher's exact test for biased strand distribution p-value = 2.10e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCTATGCCGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCTCGCATT > CP006881/3552987‑3553251
|
cGCTATGCCGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCa < 3:87467/139‑1 (MQ=255)
tGCCGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGAt < 4:51044/139‑1 (MQ=255)
tGCCGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGAt < 5:167778/139‑1 (MQ=255)
ccGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATc < 6:179066/138‑1 (MQ=255)
cGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCCTCAATTAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCag < 5:179422/139‑1 (MQ=255)
aGTGGATTGAAATGCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCGTTTTTCATTGATCagaa < 8:245474/139‑1 (MQ=255)
tGGATTCAAATTCATAGCCTTTGAGGCTTTTCCATCAATGAAATGGATAAATTGTTGGTAAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCagaag < 2:160907/138‑1 (MQ=255)
aTTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTGATTGATCAGAAGAAcc > 8:275756/1‑139 (MQ=255)
tGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGGATTTTTGATTGTTAAGAAGAACCCTAGCAAGTACaatg > 8:276790/1‑135 (MQ=255)
tGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCagaa < 7:359338/117‑1 (MQ=255)
tGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCagaa > 8:359338/1‑117 (MQ=255)
actgatgCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCAcc < 7:276790/137‑1 (MQ=255)
tgCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGGGGTTTTTATTGTTGAGCAGAACCCGAGGTGGGTAAATGGAGCAG‑CCCGGGATTTTTAAAAATTTCGCccccgggt > 8:185737/1‑135 (MQ=255)
ttGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGaa < 5:123785/139‑1 (MQ=255)
cgagcgaCCATGAGGCGGTGATACCGGCTTACAATCTAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTCGCTCGTACAATGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCa < 5:226392/139‑1 (MQ=255)
agcgaCCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGa < 4:93281/139‑1 (MQ=255)
agcgaCCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGa < 8:43646/139‑1 (MQ=255)
agcgaCCCGGAGGCGCTTATACCGGCTTACCAAAGCGAAGTGGCGTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAACGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGa < 6:130540/139‑1 (MQ=255)
aGGCGCTTATACCGGCTTACCATCGAGAAGTGGCTTTTTTCATTGATCAGAATAACCGTAGCGCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGa < 7:301508/139‑1 (MQ=255)
aGGCGCTTATACCGGCTTACCATAGAGAAGTGGGGTTTTTTATTTTTAAGAAGAACCCGTGCGCGTGAAAAGAAGCCGCCCCGGATTTTGAAAAAATT‑CGCACCCCGGACAAAAAAGCGAAAAAGCAGAAAGTGTgggg > 4:30901/1‑138 (MQ=255)
aGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCTTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGa < 3:73328/139‑1 (MQ=255)
aGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGa < 7:278102/139‑1 (MQ=255)
aGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTACCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGa < 5:5234/139‑1 (MQ=255)
cgcTTATACCGGCTTACCATAGAGACGGGGCTTTTTTAATTGATCAGAAGAACCGTAGCTCGTACAAGCAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCg < 7:310142/139‑1 (MQ=255)
cTTATACCGGCTTACCATAGAGAAGTGGGGTTTTTAATTGTTCAGAAGAAACGCAGCTCGTGCAAGGAAACCC‑CCCGGGTTTTTTAAAAATTTGGCACCCAGTTCAAAAAAGCGCAAAAGCAGAAGGGGGGGGAGCggg > 8:278102/1‑138 (MQ=255)
cTTATACCGGCTTACCATAGAGAAGTGGCGTTTTTCATTGATCAGAAGAACCCTAGCTCGGACAAGGAAGCCG‑CCCGGGATTTTTAAAAAATGCGCACCCAGTACAAAAAAGCGAAAAACCAGAAGGTGTGGGCGCggg > 5:343584/1‑138 (MQ=255)
cTTATACCGGCTTACCATAGAGAAGTGGCGTTTTTAATTGTTCAGAACCACACGAGCGCGTTAAAAGAAGACG‑CCCCGGATTTTATAAAAATTAGCACCCGTGAAAAAAAAACGAAAAAACAGAAAGTGGTGGGGCGGt > 4:73328/1‑139 (MQ=255)
gCTTACCATAGAGAAGTGGGGTTTTTGATTGATCAGAAGTACCGGAGCGGGTACAAGGCAGCCG‑CCCGGGATTTGAAAAAAATTCGCCCCCGGGTCCACAAAGCGCCAAAGCAGAAGGTGGGGGGGCGGGATATTCatc > 6:186591/1‑137 (MQ=255)
cgagaAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCACGTACCAGTAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCt < 3:214999/138‑1 (MQ=255)
gagtAGTGGCTTTTTTCATTGATCATAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCt < 2:265193/138‑1 (MQ=255)
gagaAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCt < 4:138362/138‑1 (MQ=255)
gagaAGGGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCt < 3:81429/138‑1 (MQ=255)
atgttgCTTTTTTCATTTATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCTCGCa < 7:275756/134‑1 (MQ=255)
aaGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCTCGCa < 7:17624/139‑1 (MQ=255)
tGGCGTTTTTAATTTATAAGAAGAAACCTAGCTCGGAACAAGAAGCCG‑CCCGGTATTTTAAAAAATTTCGCACCCAGTACAAAAAAGCCAAAAAACAAAAAGTGTTGGAGCGGGATAATAAGCTGCTGAGCTCGCAtt > 8:275817/1‑138 (MQ=255)
|
CGCTATGCCGAGTGGATTGAAATTCATAGCCTTGTCGGCTTTTCCATCAATGAAATGGATAAAATGATGCTCAAGGAAATTGCAGCGAGCGACCCTGAGGCGCTTATACCGGCTTACCATAGAGAAGTGGCTTTTTTCATTGATCAGAAGAACCGTAGCTCGTACAAGGAAGCCG‑CCCGGTATTTGAAAAAATTGCGCACCCTGTACAAAAAAGCGAAAAAGCAGAAGGTGTGGGAGCGGTATATTCAGCTGCTGAGCTCGCATT > CP006881/3552987‑3553251
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
