Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F56 I0 R1	218	83.1	3292392	95.3%	3137649	126.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,105,253	T→G	22.9%	F805V (TTT→GTT)	U712_05435 →	ATP‑dependent helicase/nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,105,253	0	T	G	22.9%	56.7 / 6.5	42	F805V (TTT→GTT)	U712_05435	ATP‑dependent helicase/nuclease subunit A
Reads supporting (aligned to +/- strand): ref base T (14/18); new base G (8/2); total (22/20)
Fisher's exact test for biased strand distribution p-value = 7.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGA  >  CP006881/1105121‑1105373

ttCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGCTCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGttg                                                                                                                    >  7:84054/1‑137 (MQ=255)

 tCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAg                                                                                                                    <  3:332628/138‑1 (MQ=255)

              cGAACTCATGCAGGAGCGGGTCGAAGATCTTGGGACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCGGCTTAATGACGAACCACAGCAGCAAAGGCCTCGAATTTCCAGTCGGGTTAGTAGCAGGGCTCGGCCg                                                                                                       <  5:110774/138‑1 (MQ=255)

                  cGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAAtt                                                                                                  >  3:372098/1‑139 (MQ=255)

                  cGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGTGTGCGCCggcttt                                                                                                  >  1:356127/1‑135 (MQ=255)

                       gCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCaa                                                                                              >  7:83496/1‑138 (MQ=255)

                              cGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCGAGTCGTGGGTGTGGGAGGGCTCGGGCGGCGTTTGAACaacgtgg                                                                                      >  3:259209/1‑133 (MQ=255)

                               ggggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGa                                                                                     >  6:79988/1‑139 (MQ=255)

                                 ggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAg                                                                                                                    <  8:208830/106‑1 (MQ=255)

                                 ggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAg                                                                                                                    >  7:208830/1‑106 (MQ=255)

                                 ggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTGAACATGATGGAtt                                                                                   >  8:100840/1‑139 (MQ=255)

                                 ggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGTGTCGGCCGGCATTTCAACATTATGGAtt                                                                                   >  5:27314/1‑139 (MQ=255)

                                   cGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGGTGCAGGTGTCGGCCGGGATTTCAACATGATGGCTTTg                                                                                 >  2:189056/1‑139 (MQ=255)

                                           ttGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCCACATGATGGATTTGAACAAATc                                                                         >  4:173145/1‑139 (MQ=255)

                                                  ggCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACctg                                                                  <  8:44253/139‑1 (MQ=255)

                                                  agagacagCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACctg                                                                  <  5:203432/133‑1 (MQ=255)

                                                         gcgcTCAGCGAGCAGGAGGATGTTGTCCGCGTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATa                                                           <  8:262687/139‑1 (MQ=255)

                                                            cTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAgg                                                        >  7:206248/1‑139 (MQ=255)

                                                                 cGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTc                                                   <  5:66373/139‑1 (MQ=255)

                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                         <  8:83496/139‑1 (MQ=255)

                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGTCTCGGCCGGACTTTCAAAATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGGTCGGATTTGGCa                                         >  8:302926/1‑139 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  2:223373/139‑1 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  4:372098/139‑1 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  8:206248/139‑1 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  2:4044/139‑1 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  3:29550/139‑1 (MQ=255)

                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTAAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCAc                                        >  8:200483/1‑138 (MQ=255)

                                                                              gttgtCCGTTTAATGACGATTCACAGCAGCAACCGGCTCGAATTTCCAGTCGTGTCTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                       <  8:264617/138‑1 (MQ=255)

                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTGCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGta                                  >  4:368907/1‑139 (MQ=255)

                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTGGTGTTTGTAGCAGGTCTCGGCCGGCATTTCAACATGATGGATTTGTACAAATCGTACCTGCTGGATAAGGAGCTGGGATTTGGCACGAAGta                                  >  1:201313/1‑139 (MQ=255)

                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGGCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGta                                  >  3:377660/1‑139 (MQ=255)

                                                                                          aTGACGATGCACAGCAGCTAAGGGCTCTTATTTGCAGTAGTGGTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCGGGATAAGGAGCTCGGATTTGGCACGATGTATATTCATc                          <  2:356127/139‑1 (MQ=255)

                                                                                             aCGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGGCTCGGCCGGAATTTTAACATGATGGATTTGAACAACTCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCg                        <  2:366000/138‑1 (MQ=255)

                                                                                                aTCCACAGCAGCAAAGGGCTCGAATTTGCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAAt                    <  3:368907/139‑1 (MQ=255)

                                                                                                 tCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGc                                                                                                                   >  6:409178/1‑43 (MQ=255)

                                                                                                 tCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGc                                                                                                                   <  5:409178/43‑1 (MQ=255)

                                                                                                 tCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGGCCGGATTTAAACATGATGGATTTGTAAAAAAAGGACCTGCGGGATAAGGAGCGGGGGATTTGCACGAAGTATATTAAAACGCaaa                    >  6:131268/1‑137 (MQ=255)

                                                                                                 tCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGGCTCGGGCGGAATTTAAACATGATGGATTTTAACAAATAGTACCTGCATGATAAGGAGCTAGGTTTTGGCACGAAATATATTGATCCCCAAt                    >  8:280241/1‑138 (MQ=255)

                                                                                                   cacaGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGGGGCGGGCCGAATTTCAACATGATGGCTTTGAACAAATCGTAACTGCTGGATAAGGGGCTCGGAGTTGGGACGAACTATTTTAATACGCAAATAc                 >  4:201412/1‑139 (MQ=255)

                                                                                                          gcaAATGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAg          <  7:280241/139‑1 (MQ=255)

                                                                                                           caAAGGGCTCGAATTTCCAGTCGTGTTTGTAGGAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGTTAAGGAGCTGGGATTTGGCAAGAAGTAAATTAATACCCAATTTCGAAACAGc         >  5:145178/1‑139 (MQ=255)

                                                                                                                  cTCGAATTTCCAGTCGTGGTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGa  <  5:8171/139‑1 (MQ=255)

TTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGA  >  CP006881/1105121‑1105373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A