Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F54 I0 R2 227 88.8 3424874 95.5% 3270754 127.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,444,743 T→A 20.7% N30Y (AAT→TAT)  U712_17685 ← UPF0750 membrane protein yvjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,444,7430TA20.7% 206.6 / 13.5 96N30Y (AAT→TAT) U712_17685UPF0750 membrane protein yvjA
Reads supporting (aligned to +/- strand):  ref base T (61/14);  new base A (2/19);  total (63/33)
Fisher's exact test for biased strand distribution p-value = 2.09e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATAGCATTTTAATCCTTCCC  >  CP006881/3444628‑3444879
                                                                                                                   |                                                                                                                                        
aaaGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCgctg                                                                                                                   >  2:225733/1‑139 (MQ=255)
  aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCgctgct                                                                                                                 >  6:71960/1‑139 (MQ=255)
  aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCgctgct                                                                                                                 >  7:138947/1‑139 (MQ=255)
   ggcaaatTAATAATCCACTGTACATACGCTGCTACAAAACAATATGATTGAAAAATCGTACTGACCCCGCTGACCCCGCCGGCGGAAATCTTATTCGGAAGAAAAAAAACATAAAAGCACACGGCCGTAATCGCTGCTc                                                                                                                <  7:15506/134‑1 (MQ=255)
       tatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGCCACCGCCGGCGGCACTCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAAACGCGGCTCCTAt                                                                                                            >  5:353799/1‑139 (MQ=255)
       tatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTAt                                                                                                            >  8:310884/1‑139 (MQ=255)
        atTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTAt                                                                                                            >  5:221221/1‑138 (MQ=255)
         ttAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGCCCCCGCCGGCGGCAATCTTATTCGGCAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATc                                                                                                           >  2:286895/1‑138 (MQ=255)
          aaaaaatCCACTGAACATCCGCTGATTCAAAACCATATTTTTTAAAAATCTTACTGACCCCGCTCCCCCCGCCGGCGGAAATCTTATTCGCAACAAAAAATACATAAACCCACACGGCCGTAATCGCTGCTCCTAt                                                                                                            <  4:99729/132‑1 (MQ=255)
          aaaaaatCCACTCTACATACGCTCCTAAAAAACCATATGTTTGAAAACTCGTAGTCCCCCCGCTGCCCCCGCCGGCGCCAATATTTTTCCTAACAAAAAAAACAAAACAGCCCACGGCCGTAATCGCTGCTCCTAt                                                                                                            <  4:411218/132‑1 (MQ=255)
            ataatCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGAAATCGCTGCTCCTATc                                                                                                           >  4:111026/1‑135 (MQ=255)
               atCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATg                                                                                                     >  7:7484/1‑138 (MQ=255)
               atCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGt                                                                                                    >  2:121438/1‑139 (MQ=255)
               acccACTGAAAATAGGCTGCTTCAAAACCTTATGATTTAAAATTCGTAGTGAGCCGGCTGCCCCCGCCGGGGGAAATCTTTTTCGTACCCAAAAATACATAAATGGCCACGGCCGTAATCGCTGCTCCTATCAata                                                                                                       <  4:197362/134‑1 (MQ=255)
                tCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGta                                                                                                   >  1:108654/1‑139 (MQ=255)
                tCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTAGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGATCCTATCAATATTta                                                                                                   >  2:199931/1‑139 (MQ=255)
                   aCTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGta                                                                                                   >  4:76485/1‑136 (MQ=255)
                   aCTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCCCTGACCCCGCCGGCGCCAATCTTATTCGGAAGCAAAAATACATAAAAGGACACGGCCGTAATCCCTGCTCCTATaaaaaagaa                                                                                                   >  3:95586/1‑130 (MQ=255)
                     tGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACa                                                                                               >  1:201799/1‑138 (MQ=255)
                     tGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACa                                                                                               >  2:73734/1‑138 (MQ=255)
                     tGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCCCTGCTACTATCAAAATGTATACa                                                                                               >  5:127801/1‑138 (MQ=255)
                       tACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATaa                                                                                            >  5:147289/1‑139 (MQ=255)
                       tACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATa                                                                                             >  7:10003/1‑138 (MQ=255)
                        aCATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACAt                                                                                              >  3:411218/1‑136 (MQ=255)
                        aCATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCATATTCGGAAGCAAAAATACATTAAAGCACACGGCCGTAATCGCTGCTCCTATCAATAATTATACa                                                                                               >  3:276216/1‑135 (MQ=255)
                         aaTACGCTGCTTAAAAACCATTTGATTTAAAAATCGTACTTACCCCGCTCCCCCCGCGGGCGGAAATTTTTTTCTCAACAAAAAATAAATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATa                                                                                             <  4:79290/135‑1 (MQ=255)
                            aCGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTATTCGCTGCTCCTATCAATATGTATACATAATCCCg                                                                                       >  6:232892/1‑139 (MQ=255)
                            aCGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCg                                                                                       >  2:253936/1‑139 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  7:179875/1‑139 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  6:397986/1‑139 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  5:6887/1‑139 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  7:381983/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  1:302919/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  1:194775/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  1:364053/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  7:265011/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  1:392847/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  7:332346/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAACCCCGCaaa                                                                                   >  8:161456/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATAGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGTACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  7:305824/1‑138 (MQ=255)
                                 gctTCAAAACCATATGATAGTAAAATCGTACTGATCCCGCTGACCCCGCCGCCTGCAAACATATTCGGAAGCAAAAATACATTAAAAGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATACAGCaaa                                                                                   >  7:364523/1‑138 (MQ=255)
                                  ctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATATTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATACCTCa                                                                                     >  4:276170/1‑135 (MQ=255)
                                  attaaaaaCAATATTATTTAAAAATCGTACTCACCCCCCTGCCCCGGCCGGCGTAAATATTATTCTCAACAAAAAATAAATTAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   <  1:186388/133‑1 (MQ=255)
                                   aaaaaaaCAATATTATTGAAACATCGTACTCATCCCCCGGACCCCGCGCGCGTAAATCTTAATCCCAACAAAAAATACATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATc                                                                                <  5:95618/136‑1 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCCATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  3:259203/1‑135 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  3:79290/1‑136 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  3:386878/1‑136 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                                                                  >  3:174265/1‑136 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  3:140210/1‑135 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTAATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATTTATACATAATCCCGCaaa                                                                                   >  3:47088/1‑135 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCAGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATAAATAATCACGCaaa                                                                                   >  4:259729/1‑135 (MQ=255)
                                    tCAAAACCATATGATTGTAAAATCGTACTGATCCAGCTGCCCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATAAAAGGACACGGCCGAAATCGCTCCTCCTATCAATATGTATACATAATCCCGCaaa                                                                                   >  4:96911/1‑135 (MQ=255)
                                       acaCCATATGATTTAAAAATCGTACTGACCCCGCTGACCCCGCCGGCGGAAATCTTATTCGCAACAAAAAATAAATTAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                                                            <  2:34516/137‑1 (MQ=255)
                                       aaaCCATATTATTTTAAAATCGTACTGATCCCGCTGCCCCCGCCGGGGTCATTCTTATTCGGAACAAAAAAAACAAAAAAGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                                                            <  8:10003/139‑1 (MQ=255)
                                       aaaCCATATCATTCTAAACTCGAACGTACCCCGCCCACCCCGCGGGCGCCAATATAATTCGCAACAAAAAAAAAATAACCGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                                                            <  1:121438/139‑1 (MQ=255)
                                       aaaCAATTTTATTGTAAACTGGCACTCCCCCCGCGCCCCCCGCCGGCGGAAATTTTTTTCTCAAAAAAAAATAAATTAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATcc                                                                               <  4:259203/136‑1 (MQ=255)
                                       aaaCAATTTGTTTTTAAACTCGTACGCACCCCGCGCCCCCCGCCGGCGCCAATCTTATTCGCAACAAAAAATAAATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                                                            <  8:25720/139‑1 (MQ=255)
                                       aaaCAATATGATTCTAAAATCTTACTGACCCCGCTGACCGGCCCGGCGTAAATTTTATTCTGAACAAAAAATACATACCCGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                                                            <  6:207671/139‑1 (MQ=255)
                                            atatGATTGTAAAATCGTACTGCTCCCGCTGCCCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGtt                                                                       >  6:163614/1‑139 (MQ=255)
                                             tatGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGTCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGttt                                                                      >  7:237636/1‑139 (MQ=255)
                                             tatGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGttt                                                                      >  6:359260/1‑139 (MQ=255)
                                             tatGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGttt                                                                      >  6:394963/1‑139 (MQ=255)
                                             tatGAGTGTAAAATCGTACTGATACCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAATGACAAGGCCGTAGTCGCGGCTCCTAACAATATGTATACATAATCCCGCAAAATCCACAATGttt                                                                      >  8:392312/1‑139 (MQ=255)
                                                    gTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTcct                                                               >  1:34516/1‑138 (MQ=255)
                                                     tAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCg                                                               >  7:349113/1‑138 (MQ=255)
                                                      aaaaCCGTACTGATCCCCCTGCCCCCGCCGGCCGCAATCTTATTCGGAACAAAAAATACATAAACGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGt                                                              <  7:148606/138‑1 (MQ=255)
                                                          tCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTc                                                         <  8:7484/139‑1 (MQ=255)
                                                          tCGAACTGCCCCCGCTCCCCCCGCGGGCGGAAATCTTATTCGCAACAAAAAATACAAAACCGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTc                                                         <  1:359011/139‑1 (MQ=255)
                                                           cctACTCATCCCGCTGACCCCGCGGGCCGAAATCTTATTCGTAAGAAAAAATAAATTACCCCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACg                                                           <  3:285429/134‑1 (MQ=255)
                                                           cGTACTGAGCCCCCGCCCCCCGCCGCGGGAAATCTTATTCGCAACAAAAAATACAATCAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTcc                                                        <  1:15562/139‑1 (MQ=255)
                                                            gTACTGAGCCCGCTGCCCCCGCCGGCGGCAATCTTATTCGCAAGAAAAAATACATTAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTcc                                                        <  1:225733/138‑1 (MQ=255)
                                                             tACTGATCCCGCTGCCCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATACCGCAAAATCCAAAATGTTTTATTCCGTACGTccc                                                       >  8:158439/1‑137 (MQ=255)
                                                             tACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCcat                                                      >  6:45726/1‑139 (MQ=255)
                                                              ccccaccccGCTCCCCCCGCCGGCGCAAATATTAACCGCAACAAAAAATAAAATACCCCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTcc                                                        <  2:194775/130‑1 (MQ=255)
                                                              actttgcccGCTGACCCCGCCTGCGGAAATATTATTGGGAACCAAAAATACATAAAAGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCcat                                                      <  8:265011/132‑1 (MQ=255)
                                                                  cccccGCTCACCCCGCCGGCGGAAATATTATTCGAAACAAAAAATACATAAACGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCcatca                                                    <  3:111026/134‑1 (MQ=255)
                                                                  aTCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCAATCAATAATTATACATAATCCCGCAAAATCCACAATGATTTATTCAGTACGTACATCata                                                  >  1:202275/1‑138 (MQ=255)
                                                                    cccGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCatatat                                               >  5:404679/1‑139 (MQ=255)
                                                                     ccGGTGACCCCGCCGGCGTCAATATTATTCGCAAGAAAAAATAAATTAAAGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCatat                                                 <  4:174265/136‑1 (MQ=255)
                                                                       gCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATAtcct                                            >  6:130899/1‑139 (MQ=255)
                                                                       gCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTAACAATATGTTTACATAATACCGCAAAATCCACAATGTTTTATTCCGTACGTCcatcatctg                                                >  3:348593/1‑132 (MQ=255)
                                                                          gACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATAtcct                                            >  4:246185/1‑136 (MQ=255)
                                                                           aCCCCGCCGGCGGCAATCTTATTCGCAACAAAAAATACATAACTGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGt                                        <  6:200232/139‑1 (MQ=255)
                                                                            ccccGCCGGCTGCAATTTTATTCTCAATAAAAAAAAAATAAACGCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGtt                                       <  8:179875/139‑1 (MQ=255)
                                                                            ccccGCCGGCGCCAATCTTATTCGCAACAAAAAAAACATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGtt                                       <  8:379403/139‑1 (MQ=255)
                                                                            ccccGCCGGCGCAAATCTTATTCGCAATAAAAAATACAAAAACCCACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGtt                                       <  2:201799/139‑1 (MQ=255)
                                                                            ccccGCCGGAGGCAATATTATTCGTAAGAAAAAATACATTAAAGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGtt                                       <  8:349113/139‑1 (MQ=255)
                                                                                gccgGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCGATCAATATTTATACATAATACCGCAAAATCCCCAATGTTTTATTCCGTACGTCCCTCATATATACTCCGtgtgtt                                   >  2:417692/1‑134 (MQ=255)
                                                                                    gcgtaaaTATTATTCGAAAAAAAAAATAAATAACAGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCaa                               <  8:332346/134‑1 (MQ=255)
                                                                                      ggcAATCTTATTCGGACCCAAAAATACATTAACCGCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCa                                <  4:386878/136‑1 (MQ=255)
                                                                                               aTTCGCAAGAAAAAATACATTAAAGGCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCtctc                        <  4:202071/135‑1 (MQ=255)
                                                                                                ttCGGAATCAAAAATATATTAATGGACACGGCCGTAATCGCTGCTCCTATCAATTTGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTTCGTCCATCATATATCCTCCGTTTCTTCGAATGCTCTCCGATa                   <  8:305824/139‑1 (MQ=255)
                                                                                                ttCGGAATCAAAAAAACATTAACGGCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATa                   <  2:302919/139‑1 (MQ=255)
                                                                                                     aaGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATAGCAtt              >  7:191460/1‑139 (MQ=255)
                                                                                                        cAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATAGCAtt              >  3:302539/1‑136 (MQ=255)
                                                                                                                 aTTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATAGCATTTTAATCCTTccc  >  8:57978/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                        
AAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCTCCGTTTCTTCCAATGCTCTCCGATAGCATTTTAATCCTTCCC  >  CP006881/3444628‑3444879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A