Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F59 I0 R4
|
226 |
76.6 |
2995274 |
95.4% |
2857491 |
127.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
1,653,788 |
G→A |
37.9% |
G211D (GGC→GAC) |
U712_08495 → |
GTP‑sensing transcriptional pleiotropic repressor CodY |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 1,653,788 | 0 | G | A | 37.9%
| 53.1
/ 58.7
| 58 | G211D (GGC→GAC) | U712_08495 | GTP‑sensing transcriptional pleiotropic repressor CodY |
| Reads supporting (aligned to +/- strand): ref base G (13/23); new base A (6/16); total (19/39) |
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01 |
GAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGA > CP006881/1653656‑1653917
|
gaagcaaAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTAc < 7:233408/139‑1 (MQ=255)
gcaaAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCg < 2:246564/139‑1 (MQ=255)
gcaaAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCg < 3:70633/139‑1 (MQ=255)
aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt < 5:157511/139‑1 (MQ=255)
aaaGCTGTCGTACAAATGGCTAGCAGCTCGCGTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt < 7:161135/139‑1 (MQ=255)
aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGGGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCt > 1:199618/1‑139 (MQ=255)
cAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATtgtg > 7:160139/1‑138 (MQ=255)
tttCAGCTCGCTTTCTTACAGGGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCGTGACGGAAATGAAGGTCTTCTGGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTGCGGTTATTGTGAACGCACt > 7:56247/3‑138 (MQ=255)
tATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGGCCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACt > 7:602/1‑138 (MQ=255)
tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCa < 3:357082/138‑1 (MQ=255)
aGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGa < 8:172877/138‑1 (MQ=255)
gCTCGCTTTCTTACAGTGAGCTTGAAGCAATTCAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGAAATTACCCGTTCTGTTATTGTGAACCCACTCAGa > 8:287442/1‑137 (MQ=255)
gCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCa > 5:255410/1‑131 (MQ=255)
gCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCa < 6:255410/131‑1 (MQ=255)
gCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTg < 8:221282/139‑1 (MQ=255)
aCAGTGAGGTTGAAGCAATTGAGCACATTTTTGAGGAGCTTTACGGAAATTAAGGTCTTCTTGTTGCAATTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGagag < 4:95719/136‑1 (MQ=255)
cAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGGGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc < 2:199618/138‑1 (MQ=255)
tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt > 3:277517/1‑139 (MQ=255)
tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga < 7:355919/129‑1 (MQ=255)
tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga > 8:355919/1‑129 (MQ=255)
tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt > 6:294836/1‑139 (MQ=255)
aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt > 3:90759/1‑96 (MQ=255)
aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt < 4:90759/96‑1 (MQ=255)
aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt > 7:20718/1‑139 (MQ=255)
aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCTCCGgtct > 3:243707/1‑137 (MQ=255)
aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt > 7:12000/1‑139 (MQ=255)
ttGTAGTAATTGATCACATTTTTGAGGAGCTTGTCGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTTACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGTAGAGCGCCGGTGTTAt < 4:277517/139‑1 (MQ=255)
gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt < 2:90902/137‑1 (MQ=255)
gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt < 8:160139/137‑1 (MQ=255)
gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt < 1:9546/137‑1 (MQ=255)
aTTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc > 6:153368/1‑137 (MQ=255)
ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGCGCGCCGGTGTTATCGAGTCTAg < 3:242253/139‑1 (MQ=255)
aGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGAt < 5:144998/138‑1 (MQ=255)
cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAt < 6:295493/139‑1 (MQ=255)
cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc < 1:276175/126‑1 (MQ=255)
cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc > 2:276175/1‑126 (MQ=255)
acaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAtt < 2:148390/139‑1 (MQ=255)
acaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAtt < 8:20718/139‑1 (MQ=255)
caTTTTTGAGGAGTTTGATGGAAATGAATGTTTTCTTGTTGGAAGTAAAATTGCTGACCGTGTCGGCATTACCTGTTCTTTTATTGTGAACGCACTGTGAAAGGTGTAGATGGCCGGTTTTTTCGAGTATAGATCATTa < 6:128141/139‑1 (MQ=255)
tttGAGGAGCTTGACGTAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGATGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGAACTCAGAAAGCTGTAGAGCGCCTGTGTTATCGAGTCTATATCATTAGGa < 7:287442/138‑1 (MQ=255)
tttGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGGCCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGa < 8:602/138‑1 (MQ=255)
aggggCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 8:319585/139‑1 (MQ=255)
acgagCTTTACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCAGATCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 2:57917/137‑1 (MQ=255)
gagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg < 8:12000/139‑1 (MQ=255)
gagCTTGAAGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTTCTGACCGTGTCGGGATTAGCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg < 2:168353/139‑1 (MQ=255)
agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAgg > 4:225900/1‑139 (MQ=255)
cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGa < 2:9821/87‑1 (MQ=255)
cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGa > 1:9821/1‑87 (MQ=255)
ttGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTAc < 3:206212/139‑1 (MQ=255)
gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTAc < 5:294836/137‑1 (MQ=255)
aaaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt > 2:67763/1‑56 (MQ=255)
aaaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt < 1:67763/56‑1 (MQ=255)
aaTGCAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa < 7:161013/139‑1 (MQ=255)
aaTGAAGGTCTTCTTGATGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa < 5:243861/139‑1 (MQ=255)
cttcttGTTGCAACTAAAATTGCTGACCGTGTCGGCAATACCCGTACTGATATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGAGTTATCGAGTCTAGATCATTAGGAATGAAAGCTACTTATATCAAGGTACTa > 6:56240/1‑138 (MQ=255)
gttgCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAaacaac < 3:225900/138‑1 (MQ=255)
aaaTTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTaa < 4:150296/138‑1 (MQ=255)
ccGTGTAGACATTACCCGTTCTGTTATTGTGAACGCACTCAGTTAGCTGGAGAGCGCCGGTTTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGa < 4:243707/138‑1 (MQ=255)
|
GAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGA > CP006881/1653656‑1653917
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
